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PageRuler™
Prestained Protein Ladder (10 – 170 kDa)
* Migration patterns were determined using respective NuPAGE®
precast gels.
Note. The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis conditions.
Migration patterns of PageRuler™
Prestained Protein Ladder in different electrophoresis conditions
Gel type Tris-Glycine Tris-Acetate* Bis-Tris*
Gel concentration 4-20% 8-16% 10-20% 8% 10% 12% 15% 3-8% 7% 4-12% 10% 12%
Running buffer Tris-Glycine Tris-Acetate MOPS MES MOPS MES MOPS MES
Apparent Molecular Weights, kDa
10
20
30
40
50
60
70
80
90
100
%lenghtofgel
170
130
100
70
55
40
35
25
15
10
170
130
100
70
55
40
35
25
15
10
170
130
100
70
55
40
35
25
15
10
170
130
100
70
55
40
35
25
15
170
130
100
70
55
40
35
25
15
10
170
130
100
70
55
40
35
25
15
10
170
130
100
70
55
40
35
25
15
10
150
120
85
65
50
40
30
25
150
120
85
65
50
40
30
25
140
115
80
65
50
40
30
25
15
10
140
115
80
65
50
40
30
25
15
140
115
80
65
50
40
30
25
15
140
115
80
70
50
40
30
25
15
10
140
115
80
70
50
40
30
25
15
10
140
115
80
70
50
40
30
25
15
10
LabAid™
© 2010 Fermentas www.thermoscientific.com/fermentas
Part of Thermo Fisher Scientific
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o
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PageRuler™
Prestained Protein Ladder (10 – 170 kDa)
LabAid™
, PageBlue™
, PageRuler™
, PageSilver™
are Fermentas trademarks. NuPAGE®
is registered trademark of Invitrogen Corporation.
Product Cat. # Amount
Applications MW range,
kDa
PageBlue™
staining
(#R0571)
PageSilver™
staining
(#K0681)mini-gels, 5 µl/well large gels, 10 µl/well
PageRuler™
Prestained Protein Ladder SM0671 2x250 µl 100 50 10-170 compatible compatible
Features
•	 Sharp bands
•	 Stable colors
•	 Bright reference bands
•	 Broad range
•	 Ready-to-use – supplied in a loading
buffer for direct loading on gels
Applications
•	 Monitoring protein migration during
SDS-polyacrylamide gel electrophoresis
•	 Monitoring protein transfer onto
membranes after Western blotting
•	 Sizing of proteins on SDS-polyacrylamide
gels and Western blots
Recommendations for Loading:
Step1: Thaw the Ladder either at room temperature
or at 37°C for a few minutes to dissolve
precipitated solids.
Step2: Mix gently, but throughly, to ensure that the
solution is homogeneous.
Step3: Load on SDS-polyacrylamide gel
for mini-gels with a thickness of
0.75-1 mm: 5 µl/well*
for large gels with a thickness of
0.75-1 mm: 10 µl/well*
* For thicker gels, the loading volume should be increased.
PageRuler™
Prestained
Protein Ladder, #SM0671
4-20% Tris-glycine SDS-PAGE
kDa
Blot
Gel
~35
~100
~70
~130
~170
~55
~25
~40
~15
~10