Fotoinhibice fotosyntézy
fluorometrické metody stanovení
M. Barták
Metody fluorescence chlorofylu
Jaro 2014

Textové pole: http://www.esf.edu/efb/schulz/Limnology/phytoecol1.html
http://www.esf.edu/efb/schulz/Limnology/phytoecol1.html
PI2

http://www.jeffs251lab.com/category/lab4/
Photosynthesis-Curve1


Basic de-excitation mechanisms of Chl a
n
Chl a*
Photosynth.process (linear transport e- )
Thermal dissipation
Regulation between PSII and PSI
Chlorophyll fluorescence

Reversible transport
n                                      Qa
n     3(P680+ Pheo-)          1(P680+ Pheo-)
n
n
n          3 P680*                                   1 P680*
n
n3 B carotene*    P 680                 Fluorescence
n                   O2*
n3 B carotene*
n
3Chla*           Chla*
heat
heat
Singlet
Recombin.
Charge
separation
Triplet
Recomb.
Antennae q.
Photosynthetic pathway

Metody stanovení míry fotoinhibice
nKautského křivka fluorescence chlorofylu doplněná o zhášecí mechanismy
n
nČasové řady parametrů fluorescence chlorofylu

http://www.clr.pdx.edu/projects/lakes/waldo.php
time-series-FVFm


puddlelake
qP
= (Fm’- F) / (Fm’-Fo’)
NPQ
= (Fm - Fm’) / (Fm’)
qN
= qE + qT + qI
qE

n
fig1


n
fig2


n
fig3


http://www.optisci.com/cf.htm
qT
= Fm’ after rapid relaxation is complete usually with the actinic light turned off usually one hour
- Fm’ at qE /Fm’ at steady state.
qI = Fm-Fm’ at qT/ Fm’ at steady state.
qN = Fm - Fm’/ Fm-Fo
qL = qP(Fo’/F’)
Y(NO)  = 1/NPQ +1 + qL((Fm/Fo)-1)
Y(NPQ)  = 1 - Y - Y(NO)
1 = qL + Y(NPQ) + Y(NO)
qP
= (Fm’- F) / (Fm’-Fo’)
NPQ
= (Fm - Fm’) / (Fm’)
qN
= qE + qT + qI
qE

http://www.biol.s.u-tokyo.ac.jp/users/seitaipl/personal/terashima/terashima_e.html
fig5


Brno, Laboratoř fotosyntetických procesů
nSenzitvita vůči dlouhotrvající fotoinhibici
nA zotavení se
v

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