skupina 2 skupina 1 skupina 1 skupina 2 skupina 3 skupina 3 Jonáš + Eliška Zuzka + Anička Jana + Jarka Pozitivní kontrola Eva + Veronika Amrita + Marie? Libor + Radim - k dispozici máte max. 100 mg alachloru " - k dispozici máte chlorid kademnatý CdCl2, zadané koncentrace v ředicích řadách udávají koncentraci kadmia v testu" - k dispozici máte max. 100 mg chlorpyrifosu " - k dispozici máte dichroman draselný K2Cr2O7, zadané koncentrace v ředicích řadách udávají koncentraci dichromanu draselného v testu" - k dispozici máte max. 100 mg paraquat dichloridu - k dispozici máte max. 1g triclosanu - k dispozici máte max. 100 mg dodinu 1. Alachlor 2. Cadmium chloride 3. Chlorpyrifos PC - Chromium - potassium dichromate 4. Paraquat dichloride 5. Triclosan 6. Dodine Sources CAS number 15972-60-8 10108-64-2 2921-88-2 7778-50-9 1910-42-5 3380-34-5 2439-10-3 a http://wirud-ingredients.com/certificates/subs/8_1.html Molecular formula C14H20ClNO2 CdCl2 C9H11Cl3NO3PS K2Cr2O7 C12H14Cl2N2 C12H7Cl3O2 C15H33N3O2 aa http://www.chemicalbook.com/ProductChemicalPropertiesCB6771432_EN.htm Molecular mass [g/mol] 269.77 183.32 350.59 294.19 257.16 289.54 287.44 b http://www.inchem.org/documents/icsc/icsc/eics0005.htm Density [g/cm3] 1.12 g/cm3 4.047 g/cm3 1.398 g/cm3 (43.5 °C) 2.676 g/cm3 1.25 g/cm3 1.49 g/cm3 0.9±0.1 g/cm3 Water solubility; 20°C [g/l] 0.24g 1400i 1.1e-3c 125m 620b 0.01a 0.63t c http://ec.europa.eu/food/plant/protection/evaluation/existactive/list_chlorpyrifos.pdf Vapour pressure; 20°C [Pa] < 2.9e-3g insignificantj 1.43e-3c insignificantn <0.0001b 7e-4a 1.3t d http://www.ellagret.gr/images/products/1248255789-CHLORPYRIFOS%20AGRODAN%2048%20EC%20MSDS.pdf Boiling point [°C] 100g 967i 170 - 180c 500 NR 120 e https://circabc.europa.eu/sd/d/83797096-6bc1-440f-8c35-561919feb2bf/Propiconazole_PT09_draft AR.pdf Melting point [°C] 41g 568i 41-42c 398 >300b 56 - 58a 136t f http://www.capl.sci.eg/ActiveIngredient/Propiconazole.html Kow (log P) 3.09g NR 4.7c NR - 4.5b 4.8a -0.88u g http://sitem.herts.ac.uk/aeru/ppdb/en/17.htm Safety information = R-/S- statements "R: 22-40-43-50/53-67-65-38-11-52/53-39/23/24/25-23/24/25-51/53, S: 36/37-46-60-61-62-45 h" "R: 45-46-60-61-25-26-48/23/25-51/53-50/53-48/20/22-23-22 , S: 53-45-61-60-36/37-28 j" "R20/22-65-38- 50/53, S24-36/37- 46-60-61d" "R: 45-46-60-61-8-21-25-26-34-42/43-48/23-50/53-52/53-20-48/20-23-51/53-22-36/37/38 , S: 53-45-60-61-36/37-23-26 m" R: 24/25-26-36/37/38-48/25-50/53; S: (1/2)-22-28-36/37/39-45-60-61 b "R: 36/38-50/53-36/37/38 , S: 26-39-46-60-61-24/25-22-36 aa" "R22-R36/38-R50/53, S26-S60-S61" h http://www.chemicalbook.com/ChemicalProductProperty_US_CB2437008.aspx EFFECT CONCENTRATIONS in ug/L from US EPA ECOTOX Database i http://ces.iisc.ernet.in/energy/HC270799/HDL/ENV/enven/vol319.htm#cadmium EC50 : Raphidocelis subcapitata* 19.68 4045.67** 4060 3051.10 32.71 0.53-4.46 900.00 j http://www.chemicalbook.com/ChemicalProductProperty_EN_CB9687792.htm EC50 : Daphnia magna 21850.00 72.97 0.93 374.40 2430.46 301.39 39.41 k http://www.chemicalbook.com/ProductChemicalPropertiesCB0396495_EN.htm EC50: Vibrio fischeri 128730* 23220* 2840* 333700*(6000**) 14800*(347000**) 280* 862.32* n http://www.acs.org/content/dam/acsorg/about/governance/committees/chemicalsafety/safetypractices/clip-potassium-dichromate.pdf CONCENTRATIONS TO BE TESTED mg/L or ug/L and DF=Dilution Factor CONCENTRATION OF CADMIUM CONCENTRATION OF POTASSIUM DICHROMATE Raphidocelis subcapitata (green algae) 0.64-3.2-16-80-400 ug/L (DF=5) 0.625-1.25-2.5-5-10 mg/L (DF=2) 0.04-0.2-1-5-25 mg/L (DF=5) 1.25-2.5-5-10 mg/L (DF=2) 0.004-0.02-0.1-0.5-2.5 mg/L (DF=5) 0.4-2-10-50-250 ug/L (DF=5) 0.25-0.5-1-2-4 mg/L (DF=2) o http://www.chemicalbook.com/ProductChemicalPropertiesCB7854099_EN.htm Daphnia magna (invertebrates) 6.25-12.5-25-50-100 mg/L (DF=2) 1.6-8-40-200-1000 ug/L (DF=5) 0.016-0.08-0.4-2-10 ug/L (DF=5) 0.05-0.1-0.2-0.4-0.8 mg/L (DF=2) 0.625-1.25-2.5-5-10 mg/L (DF=2) 62.5-125-250-500-1000 ug/L (DF=2) 1.6-8-40-200-1000 ug/L (DF=5) p http://www.cdpr.ca.gov/docs/risk/rcd/atrazine.pdf Vibrio fischeri (bacteria) 12.5-25-50-100-200mg/L (DF=2) 3.125-6.25-12.5-25-50 mg/L (DF=2) 0.625-1.25-2.5-5-10 mg/L (DF=2) 53 mg/L 3.125-6.25-12.5-25-50 mg/L (DF=2) 1.6-8-40-200-1000 ug/L (DF=5) 0.25-0.5-1-2-4 mg/L (DF=2) q http://www.chemicalbook.com/ChemicalProductProperty_US_CB6349448.aspx Notes: - s http://datasheets.scbt.com/sc-202581.pdf "* experimental data RECETOX, December 2013, Zuzana Rabova " "*Bonnet, J., Bonnemoy, F., Dusser, M., Bohatier, J., & Pascal, B. (2007). Assessment of the Potential Toxicity of Herbicides and Their Degradation Products to Nontarget Cells Using Two Microorganisms , the Bacteria Vibrio fischeri and the Ciliate Tetrahymena pyriformis, 78–91. doi:10.1002/tox" "*Macken, A., Giltrap, M., Ryall, K., Foley, B., McGovern, E., McHugh, B., & Davoren, M. (2009). A test battery approach to the ecotoxicological evaluation of cadmium and copper employing a battery of marine bioassays. Ecotoxicology (London, England), 18(4), 470–80. doi:10.1007/s10646-009-0305-6" "*Palma, P., Palma, V. L., Fernandes, R. M., Soares, a M. V. M., & Barbosa, I. R. (2008). Acute toxicity of atrazine, endosulfan sulphate and chlorpyrifos to Vibrio fischeri, Thamnocephalus platyurus and Daphnia magna, relative to their concentrations in surface waters from the Alentejo region of Portugal. Bulletin of environmental contamination and toxicology, 81(5), 485–9. doi:10.1007/s00128-008-9517-3" "*Fulladosa, E., Murat, J. C., & Villaescusa, I. (2005). Effect of cadmium(II), chromium(VI), and arsenic(V) on long-term viability- and growth-inhibition assays using Vibrio fischeri marine bacteria. Archives of environmental contamination and toxicology, 49(3), 299–306. doi:10.1007/s00244-004-0170-5" "*Barceló, D. (2006). Emerging Organic Pollutants in Waste Waters and Sludge: Vol. , Springer, p. 68, 31 Mar 2014 < http://books.google.cz/books?id=kbYG-dQsQYsC&pg=PA68&lpg=PA68&dq=paraquat+vibrio+fischeri+EC50&source=bl&ots=PA-SyALFXp&sig=-MUOaMwPdB9PGVo4Pz6oiAFR4cU&hl=en&sa=X&ei=21s5U6-ZGILiywPAyoKADw&ved=0CD4Q6AEwAg#v=onepage&q=paraquat%20vibrio%20fischeri%20EC50&f=false> " "*Farré, M., Asperger, D., Kantiani, L., González, S., Petrovic, M., & Barceló, D. (2008). Assessment of the acute toxicity of triclosan and methyl triclosan in wastewater based on the bioluminescence inhibition of Vibrio fischeri. Analytical and bioanalytical chemistry, 390(8), 1999–2007. doi:10.1007/s00216-007-1779-9" "* EC50 approximated from EC50 for Vibrio qinghaiensis reported in: Liu, S.-S., Wang, C.-L., Zhang, J., Zhu, X.-W., & Li, W.-Y. (2013). Combined toxicity of pesticide mixtures on green algae and photobacteria. Ecotoxicology and environmental safety, 95, 98–103. doi:10.1016/j.ecoenv.2013.05.018" t http://extoxnet.orst.edu/pips/dodine.htm **Rapidocelis EC50: Cd 2480 "** experimental data RECETOX, Petr Masner " "**Van der Schalie, W. H., James, R. R., & Gargan, T. P. (2006). Selection of a battery of rapid toxicity sensors for drinking water evaluation. Biosensors & bioelectronics, 22(1), 18–27. doi:10.1016/j.bios.2005.11.019"