Well Sample Name Detector Task Ct Qty A1 NTC GAPDH Undetermined A1 NTC cycD1 Undetermined A2 NTC GAPDH Undetermined A2 NTC cycD1 Undetermined A3 NTC GAPDH Undetermined A3 NTC cycD1 Undetermined A4 no RT GAPDH Undetermined A4 no RT cycD1 Undetermined A5 no RT GAPDH Undetermined A5 no RT cycD1 Undetermined A6 no RT GAPDH Undetermined A6 no RT cycD1 Undetermined A7 GAPDH Standard 18.9328 150 A7 cycD1 Standard 30.4351 150 A8 GAPDH Standard 18.8858 150 A8 cycD1 Standard 30.213 150 A9 GAPDH Standard 18.9542 150 A9 cycD1 Standard 30.204 150 A10 GAPDH Standard 22.3117 15 A10 cycD1 Standard 33.5172 15 A11 GAPDH Standard 22.4113 15 A11 cycD1 Standard 33.6619 15 A12 GAPDH Standard 22.319 15 A12 cycD1 Standard 33.9236 15 B1 GAPDH Standard 25.1238 1.5 B1 cycD1 Standard 36.8315 1.5 B2 GAPDH Standard 25.9883 1.5 B2 cycD1 Standard 38.4694 1.5 B3 GAPDH Standard 26.2075 1.5 B3 cycD1 Standard 37.1088 1.5 B4 GAPDH Standard 32.3466 0.15 B4 cycD1 Standard Undetermined 0.15 B5 GAPDH Standard 32.0712 0.15 B5 cycD1 Standard Undetermined 0.15 B6 GAPDH Standard 32.2291 0.15 B6 cycD1 Standard Undetermined 0.15 B7 GAPDH Standard 35.9315 0.015 B7 cycD1 Standard Undetermined 0.015 B8 GAPDH Standard 36.2911 0.015 B8 cycD1 Standard Undetermined 0.015 B9 GAPDH Standard 35.5471 0.015 B9 cycD1 Standard Undetermined 0.015 B10 GAPDH Standard 38.5827 0.0015 B10 cycD1 Standard Undetermined 0.0015 B11 GAPDH Standard Undetermined 0.0015 B11 cycD1 Standard Undetermined 0.0015 B12 GAPDH Standard 39.8272 0.0015 B12 cycD1 Standard Undetermined 0.0015 C1 NTC GAPDH Undetermined C1 NTC cycD1 Undetermined C2 NTC GAPDH Undetermined C2 NTC cycD1 Undetermined C3 NTC GAPDH Undetermined C3 NTC cycD1 Undetermined C4 noRT GAPDH Undetermined C4 noRT cycD1 Undetermined C5 noRT GAPDH Undetermined C5 noRT cycD1 Undetermined C6 noRT GAPDH Undetermined C6 noRT cycD1 Undetermined C7 GAPDH Standard 20.2365 150 C7 cycD1 Standard 29.4299 150 C8 GAPDH Standard 20.2172 150 C8 cycD1 Standard 29.2361 150 C9 GAPDH Standard 20.1892 150 C9 cycD1 Standard 29.4002 150 C10 GAPDH Standard 23 15 C10 cycD1 Standard 32.2271 15 C11 GAPDH Standard 22.775 15 C11 cycD1 Standard 32.2071 15 C12 GAPDH Standard 22.9544 15 C12 cycD1 Standard 32.7141 15 D1 GAPDH Standard 24.2544 1.5 D1 cycD1 Standard 34.0914 1.5 D2 GAPDH Standard 24.8546 1.5 D2 cycD1 Standard 34.2173 1.5 D3 GAPDH Standard 24.5075 1.5 D3 cycD1 Standard 34.0017 1.5 D4 GAPDH Standard 27.3028 0.15 D4 cycD1 Standard 35.1057 0.15 D5 GAPDH Standard 27.1917 0.15 D5 cycD1 Standard 36 0.15 D6 GAPDH Standard 27.2313 0.15 D6 cycD1 Standard 35.3542 0.15 D7 GAPDH Standard 30.6562 0.015 D7 cycD1 Standard Undetermined 0.015 D8 GAPDH Standard 30.6408 0.015 D8 cycD1 Standard 37.815 0.015 D9 GAPDH Standard 30.6775 0.015 D9 cycD1 Standard 38.2941 0.015 D10 GAPDH Standard 33.7523 0.0015 D10 cycD1 Standard Undetermined 0.0015 D11 GAPDH Standard 33.879 0.0015 D11 cycD1 Standard Undetermined 0.0015 D12 GAPDH Standard 33.5282 0.0015 D12 cycD1 Standard Undetermined 0.0015 TPA: GAPDH CycD1 Vzorek Ct Vzorek Ct cDNA 21.16 cDNA 30.29 cDNA 21.34 cDNA 30.35 cDNA 21.44 cDNA 30.39 Æ Æ EtOH 1µl Undetermined EtOH 1µl Undetermined EtOH 1µl Undetermined EtOH 1µl Undetermined Æ Æ Bez činidla: GAPDH CycD1 Vzorek Ct Vzorek Ct cDNA 19.6 cDNA 31 cDNA 19.61 cDNA 30.99 cDNA 20.07 cDNA 31.35 Æ Æ EtOH 2µl 31.51 EtOH 2µl Undetermined EtOH 2µl 24 EtOH 2µl Undetermined Æ Æ ##### Sheet/List 2 ##### Document Name: skupina 2 Plate Type: Standard Curve User: Student Document Information Operator: Administrator Run Date: Tuesday May 19 2015 11:01:01 Last Modified: Tuesday May 19 2015 13:02:12 Instrument Type: Applied Biosystems 7500 Real-Time PCR System Comments: SDS v1.4 Thermal Cycler Profile Stage Repetitions Temperature Time Ramp Rate 1 1 50.0 °C 2:00 100 2 1 95.0 °C 10:00 100 3 40 95.0 °C 0:15 100 60.0 °C 1:00 100 GAPDH FBS vs. no FBS GAPDH a CDK4 4 (Dissociation) 1 95.0 °C 0:15 Auto 60.0 °C 1:00 Auto 95.0 °C 0:15 Auto 60.0 °C 0:15 Auto Standard 7500 Mode Data Collection : Stage 3 Step 2 PCR Volume: 25 µL Well Sample Name Detector Task Ct Qty A1 FBS GAPDH_VIC Standard 17.7801 150 A1 FBS CCND2 Standard 17.7113 150 A2 FBS GAPDH_VIC Standard 28.8104 15 A2 FBS CCND2 Standard 29.0249 15 A3 FBS GAPDH_VIC Standard 25.4258 1.5 A3 FBS CCND2 Standard 25.4711 1.5 A4 FBS GAPDH_VIC Standard 28.7359 0.15 A4 FBS CCND2 Standard 28.8236 0.15 A5 FBS GAPDH_VIC Standard 31.8027 0.015 A5 FBS CCND2 Standard 32.1164 0.015 A6 FBS GAPDH_VIC Standard 33.8092 0.0015 A6 FBS CCND2 Standard 34.7684 0.0015 A7 NTC GAPDH_VIC Unknown 34.1731 A7 NTC CCND2 Unknown 35.2022 A8 noRT GAPDH_VIC Unknown 34.668 CCND2 A8 noRT CCND2 Unknown 36.6071 A9 FBS GAPDH_VIC Unknown 30.8227 FBS vs. no FBS GAPDH a CCND2 A9 FBS CCND2 Unknown 30.2177 A10 FBS GAPDH_VIC Unknown 22.2657 A10 FBS CCND2 Unknown 21.8311 A11 FBS GAPDH_VIC Unknown 22.3013 A11 FBS CCND2 Unknown 21.8317 B1 FBS GAPDH_VIC Standard 17.7856 150 B1 FBS CCND2 Standard 17.8682 150 B2 FBS GAPDH_VIC Standard 20.68 15 B2 FBS CCND2 Standard 20.849 15 B3 FBS GAPDH_VIC Standard 25.3642 1.5 B3 FBS CCND2 Standard 25.3911 1.5 B4 FBS GAPDH_VIC Standard 28.8839 0.15 B4 FBS CCND2 Standard 28.9241 0.15 B5 FBS GAPDH_VIC Standard 31.9379 0.015 B5 FBS CCND2 Standard 32.1491 0.015 B6 FBS GAPDH_VIC Standard 33.788 0.0015 B6 FBS CCND2 Standard 35.0036 0.0015 B7 NTC GAPDH_VIC Unknown 34.5425 B7 NTC CCND2 Unknown 36.5256 B8 noRT GAPDH_VIC Unknown 34.9695 B8 noRT CCND2 Unknown 36.4757 B9 FBS 1 ul EtOH GAPDH_VIC Unknown 22.8377 B9 FBS 1 ul EtOH CCND2 Unknown 21.5034 B10 FBS 1 ul EtOH GAPDH_VIC Unknown 31.9272 B10 FBS 1 ul EtOH CCND2 Unknown 21.5792 B11 FBS 1 ul EtOH GAPDH_VIC Unknown 22.4141 B11 FBS 1 ul EtOH CCND2 Unknown 21.5062 CDK4 C1 FBS GAPDH_VIC Standard 17.972 150 C1 FBS CCND2 Standard 17.9635 150 C2 FBS GAPDH_VIC Standard 20.7696 15 C2 FBS CCND2 Standard 20.8682 15 Vliv inhibitorů C3 FBS GAPDH_VIC Standard 25.4548 1.5 C3 FBS CCND2 Standard 25.486 1.5 C4 FBS GAPDH_VIC Standard 28.9686 0.15 C4 FBS CCND2 Standard 28.9615 0.15 C5 FBS GAPDH_VIC Standard 32.065 0.015 C5 FBS CCND2 Standard 32.2418 0.015 C6 FBS GAPDH_VIC Standard 33.957 0.0015 C6 FBS CCND2 Standard 35.5264 0.0015 C9 FBS 2 ul EtOH GAPDH_VIC Unknown Undetermined C9 FBS 2 ul EtOH CCND2 Unknown Undetermined C10 FBS 2 ul EtOH GAPDH_VIC Unknown Undetermined C10 FBS 2 ul EtOH CCND2 Unknown Undetermined C11 FBS 2 ul EtOH GAPDH_VIC Unknown Undetermined C11 FBS 2 ul EtOH CCND2 Unknown Undetermined D1 FBS GAPDH_VIC Standard Undetermined 150 D1 FBS CDK4 Standard Undetermined 150 D2 FBS GAPDH_VIC Standard 20.7126 15 D2 FBS CDK4 Standard 21.675 15 D3 FBS GAPDH_VIC Standard 25.4033 1.5 D3 FBS CDK4 Standard 26.4056 1.5 D4 FBS GAPDH_VIC Standard 29.1394 0.15 D4 FBS CDK4 Standard 29.9519 0.15 D5 FBS GAPDH_VIC Standard 32.2298 0.015 D5 FBS CDK4 Standard 32.9015 0.015 D6 FBS GAPDH_VIC Standard 35.3008 0.0015 D6 FBS CDK4 Standard 36.9286 0.0015 D7 NTC GAPDH_VIC Unknown Undetermined D7 NTC CDK4 Unknown Undetermined D8 noRT GAPDH_VIC Unknown Undetermined D8 noRT CDK4 Unknown Undetermined D9 FBS GAPDH_VIC Unknown 22.079 D9 FBS CDK4 Unknown 22.657 D10 FBS GAPDH_VIC Unknown 22.2376 D10 FBS CDK4 Unknown 22.8231 D11 FBS GAPDH_VIC Unknown 22.0553 D11 FBS CDK4 Unknown 22.6156 E1 FBS GAPDH_VIC Standard 18.1218 150 E1 FBS CDK4 Standard 19.0387 150 E2 FBS GAPDH_VIC Standard 20.7517 15 E2 FBS CDK4 Standard 21.7599 15 E3 FBS GAPDH_VIC Standard 25.5744 1.5 E3 FBS CDK4 Standard 26.3939 1.5 E4 FBS GAPDH_VIC Standard 29.1107 0.15 E4 FBS CDK4 Standard 30.0233 0.15 E5 FBS GAPDH_VIC Standard 32.3973 0.015 E5 FBS CDK4 Standard 33.1645 0.015 E6 FBS GAPDH_VIC Standard 35.6124 0.0015 E6 FBS CDK4 Standard 37.2575 0.0015 E7 NTC GAPDH_VIC Unknown Undetermined E7 NTC CDK4 Unknown Undetermined E8 noRT GAPDH_VIC Unknown Undetermined E8 noRT CDK4 Unknown Undetermined E9 FBS 1 ul EtOH GAPDH_VIC Unknown 22.0709 E9 FBS 1 ul EtOH CDK4 Unknown 22.575 E10 FBS 1 ul EtOH GAPDH_VIC Unknown 21.9384 E10 FBS 1 ul EtOH CDK4 Unknown 22.4067 E11 FBS 1 ul EtOH GAPDH_VIC Unknown Undetermined E11 FBS 1 ul EtOH CDK4 Unknown Undetermined F1 FBS GAPDH_VIC Standard 18.0672 150 F1 FBS CDK4 Standard 18.9643 150 F2 FBS GAPDH_VIC Standard 20.9728 15 F2 FBS CDK4 Standard 21.9589 15 F3 FBS GAPDH_VIC Standard 25.6425 1.5 F3 FBS CDK4 Standard 26.4626 1.5 F4 FBS GAPDH_VIC Standard 29.1642 0.15 F4 FBS CDK4 Standard 30.0643 0.15 F5 FBS GAPDH_VIC Standard 32.266 0.015 F5 FBS CDK4 Standard 33.2807 0.015 F6 FBS GAPDH_VIC Standard 35.5929 0.0015 F6 FBS CDK4 Standard 35.9053 0.0015 F9 FBS 2 ul EtOH GAPDH_VIC Unknown Undetermined F9 FBS 2 ul EtOH CDK4 Unknown Undetermined F10 FBS 2 ul EtOH GAPDH_VIC Unknown Undetermined F10 FBS 2 ul EtOH CDK4 Unknown Undetermined F11 FBS 2 ul EtOH GAPDH_VIC Unknown Undetermined F11 FBS 2 ul EtOH CDK4 Unknown Undetermined G9 bez sera GAPDH_VIC Unknown 22.6157 G9 bez sera CCND2 Unknown 22.2307 G10 bez sera GAPDH_VIC Unknown 22.3788 G10 bez sera CCND2 Unknown 22.0872 G11 bez sera GAPDH_VIC Unknown 22.3415 G11 bez sera CCND2 Unknown 22.0627 H9 bez sera GAPDH_VIC Unknown 22.2023 H9 bez sera CDK4 Unknown 22.7495 H10 bez sera GAPDH_VIC Unknown 22.1393 H10 bez sera CDK4 Unknown 22.7188 H11 bez sera GAPDH_VIC Unknown 22.2282 H11 bez sera CDK4 Unknown 22.7198 ##### Sheet/List 3 ##### Document Name: skupina 3 Plate Type: Standard Curve User: Student Document Information Operator: Administrator Run Date: Tuesday May 19 2015 15:28:19 Last Modified: Tuesday May 19 2015 16:57:56 Instrument Type: Applied Biosystems 7500 Real-Time PCR System Comments: SDS v1.4 Thermal Cycler Profile Stage Repetitions Temperature Time Ramp Rate 1 1 50.0 °C 2:00 100 2 1 95.0 °C 10:00 100 3 40 95.0 °C 0:15 100 60.0 °C 1:00 100 4 (Dissociation) 1 95.0 °C 0:15 Auto 60.0 °C 1:00 Auto 95.0 °C 0:15 Auto 60.0 °C 0:15 Auto Standard 7500 Mode Data Collection : Stage 3 Step 2 PCR Volume: 25 µL GAPDH FBS vs. no FBS GAPDH a CCND3 Well Sample Name Detector Task Ct StdDev Ct Qty A1 GAPDH_VIC Standard 19.4175 150 A1 CCND3 Standard 23.4349 150 A2 GAPDH_VIC Standard 21.6803 15 A2 CCND3 Standard 25.8999 15 A3 GAPDH_VIC Standard 25.0957 1.5 A3 CCND3 Standard 29.2836 1.5 A4 GAPDH_VIC Standard 28.4504 0.15 A4 CCND3 Standard 32.4464 0.15 A5 GAPDH_VIC Standard 31.8103 0.015 A5 CCND3 Standard 35.3045 0.015 A6 GAPDH_VIC Standard 35.3691 0.0015 A6 CCND3 Standard Undetermined 0.0015 A7 NTC GAPDH_VIC Unknown 39.5253 A7 NTC CCND3 Unknown Undetermined A8 noRT GAPDH_VIC Unknown Undetermined A8 noRT CCND3 Unknown Undetermined A9 noRT GAPDH_VIC Unknown 39.3293 A9 noRT CCND3 Unknown Undetermined A10 bez sera GAPDH_VIC Unknown 22.2828 A10 bez sera CCND3 Unknown 26.3634 A11 bez sera GAPDH_VIC Unknown 22.3949 A11 bez sera CCND3 Unknown 26.3719 A12 bez sera GAPDH_VIC Unknown 23.0142 A12 bez sera CCND3 Unknown 26.2957 CCND3 B1 GAPDH_VIC Standard 18.6189 150 FBS vs. no FBS GAPDH a JunD B1 CCND3 Standard 22.6283 150 B2 GAPDH_VIC Standard 21.8138 15 B2 CCND3 Standard 26.0068 15 B3 GAPDH_VIC Standard 25.1659 1.5 B3 CCND3 Standard 29.3774 1.5 B4 GAPDH_VIC Standard 28.6282 0.15 B4 CCND3 Standard 32.7353 0.15 B5 GAPDH_VIC Standard 32.064 0.015 B5 CCND3 Standard 36.8365 0.015 B6 GAPDH_VIC Standard 35.2233 0.0015 B6 CCND3 Standard 39.7392 0.0015 B7 NTC GAPDH_VIC Unknown Undetermined B7 NTC JunD Unknown Undetermined B8 noRT GAPDH_VIC Unknown Undetermined B8 noRT JunD Unknown Undetermined B9 noRT GAPDH_VIC Unknown Undetermined B9 noRT JunD Unknown Undetermined B10 bez sera GAPDH_VIC Unknown 22.259 B10 bez sera JunD Unknown 26.3573 B11 bez sera GAPDH_VIC Unknown 22.3422 B11 bez sera JunD Unknown 26.3632 B12 bez sera GAPDH_VIC Unknown 22.0592 B12 bez sera JunD Unknown 26.3279 C1 GAPDH_VIC Standard 18.7298 150 C1 CCND3 Standard 22.7195 150 JunD C2 GAPDH_VIC Standard 21.8245 15 C2 CCND3 Standard 25.9791 15 C3 GAPDH_VIC Standard 24.7397 1.5 C3 CCND3 Standard 29.0607 1.5 Vliv inhibitorů C4 GAPDH_VIC Standard 28.6941 0.15 C4 CCND3 Standard 32.5152 0.15 C5 GAPDH_VIC Standard 31.4033 0.015 C5 CCND3 Standard 35.5964 0.015 C6 GAPDH_VIC Standard 35.2178 0.0015 C6 CCND3 Standard Undetermined 0.0015 C10 FBS GAPDH_VIC Unknown 22.2913 C10 FBS CCND3 Unknown 26.4279 C11 FBS GAPDH_VIC Unknown 22.2198 C11 FBS CCND3 Unknown 26.4324 C12 FBS GAPDH_VIC Unknown 22.1536 C12 FBS CCND3 Unknown 26.2809 D1 GAPDH_VIC Standard 18.8789 150 D1 JunD Standard 24.5394 150 D2 GAPDH_VIC Standard 21.8041 15 D2 JunD Standard 26.1271 15 D3 GAPDH_VIC Standard 25.1906 1.5 D3 JunD Standard 28.7587 1.5 D4 GAPDH_VIC Standard 28.6436 0.15 D4 JunD Standard 31.8381 0.15 D5 GAPDH_VIC Standard 32.1099 0.015 D5 JunD Standard 35.0348 0.015 D6 GAPDH_VIC Standard 34.9345 0.0015 D6 JunD Standard 37.8332 0.0015 D10 FBS GAPDH_VIC Unknown 22.4276 D10 FBS JunD Unknown 26.2645 D11 FBS GAPDH_VIC Unknown 22.3302 D11 FBS JunD Unknown 26.0897 D12 FBS GAPDH_VIC Unknown 22.185 D12 FBS JunD Unknown 27.5725 E1 GAPDH_VIC Standard 18.8997 150 E1 JunD Standard 24.6273 150 E2 GAPDH_VIC Standard 21.9112 15 E2 JunD Standard 25.7467 15 E3 GAPDH_VIC Standard 25.2805 1.5 E3 JunD Standard 28.993 1.5 E4 GAPDH_VIC Standard 28.6066 0.15 E4 JunD Standard 31.1467 0.15 E5 GAPDH_VIC Standard 32.1377 0.015 E5 JunD Standard 34.094 0.015 E6 GAPDH_VIC Standard 35.5773 0.0015 E6 JunD Standard 37.705 0.0015 E10 FBS 1 ul GAPDH_VIC Unknown 29.5014 E10 FBS 1 ul CCND3 Unknown 28.439 E11 FBS 1 ul GAPDH_VIC Unknown Undetermined E11 FBS 1 ul CCND3 Unknown Undetermined E12 FBS 1 ul GAPDH_VIC Unknown Undetermined E12 FBS 1 ul CCND3 Unknown Undetermined F1 GAPDH_VIC Standard 18.8186 150 F1 JunD Standard 24.312 150 F2 GAPDH_VIC Standard 21.8637 15 F2 JunD Standard 26.2044 15 F3 GAPDH_VIC Standard 25.0908 1.5 F3 JunD Standard 28.8951 1.5 F4 GAPDH_VIC Standard 28.7065 0.15 F4 JunD Standard 31.8502 0.15 F5 GAPDH_VIC Standard 32.008 0.015 F5 JunD Standard 36 0.015 F6 GAPDH_VIC Standard 35.536 0.0015 F6 JunD Standard 39.2465 0.0015 F10 FBS 1 ul GAPDH_VIC Unknown Undetermined F10 FBS 1 ul JunD Unknown Undetermined F11 FBS 1 ul GAPDH_VIC Unknown Undetermined F11 FBS 1 ul JunD Unknown Undetermined F12 FBS 1 ul GAPDH_VIC Unknown Undetermined F12 FBS 1 ul JunD Unknown Undetermined G10 FBS 2 ul GAPDH_VIC Unknown Undetermined G10 FBS 2 ul CCND3 Unknown Undetermined G11 FBS 2 ul GAPDH_VIC Unknown Undetermined G11 FBS 2 ul CCND3 Unknown Undetermined G12 FBS 2 ul GAPDH_VIC Unknown Undetermined G12 FBS 2 ul CCND3 Unknown Undetermined H10 FBS 2 ul GAPDH_VIC Unknown Undetermined H10 FBS 2 ul JunD Unknown Undetermined H11 FBS 2 ul GAPDH_VIC Unknown Undetermined H11 FBS 2 ul JunD Unknown Undetermined H12 FBS 2 ul GAPDH_VIC Unknown Undetermined H12 FBS 2 ul JunD Unknown Undetermined