c (ng/ul) log c GRAF 75 1.875061 log c GAPDH gen 1 gen 2 7.5 0.875061 1.875061 0.75 -0.1249387 0.875061 0.075 -1.1249387 -0.1249387 0.0075 -2.1249387 -1.1249387 0.00075 -3.1249387 -2.1249387 -3.1249387 GAPDH gen 1 gen 2 Vzorek Ct Vzorek Ct Vzorek Ct undil undil undil undil undil undil undil undil undil undil Æ Æ undil 10x 10x undil 10x 10x Æ 10x 10x 10x Æ Æ 10x 100x 100x 10x 100x 100x 10x 100x 100x 10x Æ Æ 10x 1000x 1000x Æ 1000x 1000x 100x 1000x 1000x 100x Æ Æ 100x 10 000x 10 000x 100x 10 000x 10 000x 100x 10 000x 10 000x 100x Æ Æ Æ 100 000x 100 000x 1000x 100 000x 100 000x 1000x 100 000x 100 000x a) GAPDH y = 1000x Æ Æ 1000x E = (10^-1/k - 1) x 100 = % 1000x 1000x Æ b) gen 1 y = 10 000x 10 000x E = (10^-1/k - 1) x 100 = % 10 000x 10 000x c) gen 2 y = 10 000x 10 000x E = (10^-1/k - 1) x 100 = % Æ 100 000x 100 000x 100 000x 100 000x 100 000x 100 000x Æ Kontroly NTC GAPDH gen 1 gen 2 noRT GAPDH gen 1 gen 2 ##### Sheet/List 2 ##### S 20% FBS DUPLEX Bez 20% FBS GAPDH gen 1 GAPDH gen 1 Vzorek Ct Vzorek Ct Vzorek Ct Vzorek Ct cDNA cDNA cDNA cDNA cDNA cDNA cDNA cDNA cDNA cDNA cDNA cDNA Æ Æ Æ Æ EtOH 1µl EtOH 1µl GAPDH gen 2 EtOH 1µl EtOH 1µl Vzorek Ct Vzorek Ct EtOH 1µl EtOH 1µl cDNA cDNA Æ Æ cDNA cDNA EtOH 2 µl EtOH 2 µl cDNA cDNA EtOH 2 µl EtOH 2 µl Æ Æ EtOH 2 µl EtOH 2 µl Æ Æ GAPDH gen 2 Vzorek Ct Vzorek Ct cDNA cDNA 1 S 20% FBS cDNA cDNA 2 Bez 20% FBS cDNA cDNA Æ Æ EtOH 1µl EtOH 1µl Vzorek gen 1 GAPDH Δ Ct ΔΔ Ct R EtOH 1µl EtOH 1µl S 20% FBS EtOH 1µl EtOH 1µl Bez 20% FBS Æ Æ Vzorek gen 2 GAPDH Δ Ct ΔΔ Ct R EtOH 2 µl EtOH 2 µl S 20% FBS EtOH 2 µl EtOH 2 µl Bez 20% FBS EtOH 2 µl EtOH 2 µl Æ Æ 1 S 20% FBS 2 Bez 20% FBS SINGLEPLEX S 20% FBS GAPDH gen 1 gen 2 Vzorek Ct Vzorek Ct Vzorek Ct Vzorek gen 1 GAPDH Δ Ct ΔΔ Ct R cDNA cDNA cDNA S 20% FBS cDNA cDNA cDNA Bez 20% FBS cDNA cDNA cDNA Vzorek gen 2 GAPDH Δ Ct ΔΔ Ct R Æ Æ Æ S 20% FBS Bez 20% FBS Bez 20% FBS GAPDH gen 1 gen 2 Vzorek Ct Vzorek Ct Vzorek Ct cDNA cDNA cDNA cDNA cDNA cDNA cDNA cDNA cDNA Æ Æ Æ 1 S 20% FBS 2 Bez 20% FBS 1 S 20% FBS 2 Bez 20% FBS