Document Name: sk.1 Plate Type: Standard Curve Kalibrační křivky Relativní kvantifikace - duplex Vliv inhibitorů User: Student GAPDH 20 % FBS vs. no FBS CCND1 a GAPDH Document Information Operator: Administrator Run Date: Tuesday May 17 2016 11:11:50 Last Modified: Tuesday May 17 2016 13:05:16 Instrument Type: Applied Biosystems 7500 Real-Time PCR System Comments: SDS v1.4 Thermal Cycler Profile Stage Repetitions Temperature Time Ramp Rate 1 1 50.0 °C 2:00 100 2 1 95.0 °C 10:00 100 3 40 95.0 °C 0:15 100 60.0 °C 1:00 100 Standard 7500 Mode Data Collection : Stage 3 Step 2 PCR Volume: 25 µL Standard Curve Information Detector Name Slope Intercept R2 Number of Standards CCND1 -3.367248 40.292183 0.995044 16 GAPDH_VIC -3.404152 36.660984 0.997967 36 CCND1 20 % FBS vs. no FBS FOS a GAPDH Well Sample Name Detector Task Ct Qty A1 CCND1 CCND1 Standard 22.4806 150 A1 CCND1 GAPDH_VIC Standard 19.1771 150 A2 CCND1 CCND1 Standard 26.2301 15 A2 CCND1 GAPDH_VIC Standard 22.1689 15 A3 CCND1 CCND1 Standard 29.8506 1.5 A3 CCND1 GAPDH_VIC Standard 25.7964 1.5 A4 CCND1 CCND1 Standard 33.2285 0.15 A4 CCND1 GAPDH_VIC Standard 29.2761 0.15 A5 CCND1 CCND1 Standard 36.5361 0.015 A5 CCND1 GAPDH_VIC Standard 32.6891 0.015 A6 CCND1 CCND1 Standard Undetermined 0.0015 A6 CCND1 GAPDH_VIC Standard 36.0633 0.0015 A7 FOS FOS Standard Undetermined 150 A7 FOS GAPDH_VIC Standard 19.0457 150 A8 FOS FOS Standard Undetermined 15 A8 FOS GAPDH_VIC Standard 21.9628 15 A9 FOS FOS Standard Undetermined 1.5 A9 FOS GAPDH_VIC Standard 25.7167 1.5 A10 FOS FOS Standard Undetermined 0.15 A10 FOS GAPDH_VIC Standard 29.3319 0.15 A11 FOS FOS Standard Undetermined 0.015 A11 FOS GAPDH_VIC Standard 32.9755 0.015 A12 FOS FOS Standard Undetermined 0.0015 A12 FOS GAPDH_VIC Standard 37.0459 0.0015 B1 CCND1 CCND1 Standard 22.4691 150 FOS Relativní kvantifikace - singlelex B1 CCND1 GAPDH_VIC Standard 19.1223 150 B2 CCND1 CCND1 Standard 26.2768 15 20 % FBS vs. no FBS CCND1 B2 CCND1 GAPDH_VIC Standard 22.2761 15 B3 CCND1 CCND1 Standard 29.546 1.5 B3 CCND1 GAPDH_VIC Standard 25.7149 1.5 B4 CCND1 CCND1 Standard 33.2655 0.15 B4 CCND1 GAPDH_VIC Standard 29.2897 0.15 B5 CCND1 CCND1 Standard 36.1893 0.015 B5 CCND1 GAPDH_VIC Standard 32.8098 0.015 B6 CCND1 CCND1 Standard Undetermined 0.0015 B6 CCND1 GAPDH_VIC Standard 36.3063 0.0015 B7 FOS FOS Standard Undetermined 150 B7 FOS GAPDH_VIC Standard 19.1185 150 B8 FOS FOS Standard Undetermined 15 B8 FOS GAPDH_VIC Standard 22.0305 15 B9 FOS FOS Standard Undetermined 1.5 B9 FOS GAPDH_VIC Standard 25.8993 1.5 B10 FOS FOS Standard Undetermined 0.15 B10 FOS GAPDH_VIC Standard 29.4035 0.15 B11 FOS FOS Standard Undetermined 0.015 B11 FOS GAPDH_VIC Standard 32.1342 0.015 B12 FOS FOS Standard Undetermined 0.0015 B12 FOS GAPDH_VIC Standard 36.659 0.0015 C1 CCND1 CCND1 Standard 22.6627 150 C1 CCND1 GAPDH_VIC Standard 19.4312 150 C2 CCND1 CCND1 Standard 26.3222 15 C2 CCND1 GAPDH_VIC Standard 22.3927 15 20 % FBS vs. no FBS FOS C3 CCND1 CCND1 Standard 29.9768 1.5 C3 CCND1 GAPDH_VIC Standard 26.0349 1.5 C4 CCND1 CCND1 Standard 33.4683 0.15 C4 CCND1 GAPDH_VIC Standard 29.5394 0.15 C5 CCND1 CCND1 Standard 36.5321 0.015 C5 CCND1 GAPDH_VIC Standard 32.8287 0.015 C6 CCND1 CCND1 Standard 39.2198 0.0015 C6 CCND1 GAPDH_VIC Standard 36.3923 0.0015 C7 FOS FOS Standard Undetermined 150 C7 FOS GAPDH_VIC Standard 19.1642 150 C8 FOS FOS Standard Undetermined 15 C8 FOS GAPDH_VIC Standard 22.1297 15 C9 FOS FOS Standard Undetermined 1.5 C9 FOS GAPDH_VIC Standard 25.9549 1.5 C10 FOS FOS Standard Undetermined 0.15 C10 FOS GAPDH_VIC Standard 29.5869 0.15 C11 FOS FOS Standard Undetermined 0.015 C11 FOS GAPDH_VIC Standard 33.2499 0.015 C12 FOS FOS Standard Undetermined 0.0015 C12 FOS GAPDH_VIC Standard 36.7203 0.0015 D1 20 % FBS CCND1 Unknown 27.0304 D2 20 % FBS CCND1 Unknown 27.1924 D3 20 % FBS CCND1 Unknown 27.2013 D4 bez FBS CCND1 Unknown 28.0167 D5 bez FBS CCND1 Unknown 27.9617 D6 bez FBS CCND1 Unknown 27.9128 20 % FBS vs. no FBS GAPDH-FAM D9 noRT CCND1 Unknown Undetermined D9 noRT GAPDH_VIC Unknown Undetermined D10 noRT FOS Unknown Undetermined D10 noRT GAPDH_VIC Unknown Undetermined D11 NK CCND1 Unknown Undetermined D11 NK GAPDH_VIC Unknown Undetermined D12 NK FOS Unknown Undetermined D12 NK GAPDH_VIC Unknown Undetermined E1 20 % FBS FOS Unknown Undetermined E2 20 % FBS FOS Unknown Undetermined E3 20 % FBS FOS Unknown Undetermined E4 bez FBS FOS Unknown Undetermined E5 bez FBS FOS Unknown Undetermined E6 bez FBS FOS Unknown Undetermined E9 noRT CCND1 Unknown Undetermined E9 noRT GAPDH_VIC Unknown Undetermined E10 noRT FOS Unknown Undetermined E10 noRT GAPDH_VIC Unknown Undetermined E11 NK CCND1 Unknown Undetermined E11 NK GAPDH_VIC Unknown Undetermined E12 NK FOS Unknown Undetermined E12 NK GAPDH_VIC Unknown Undetermined F1 20 % FBS GAPDH Unknown 21.2632 F2 20 % FBS GAPDH Unknown 21.3701 F3 20 % FBS GAPDH Unknown 21.3485 F4 bez FBS GAPDH Unknown 21.8278 F5 bez FBS GAPDH Unknown 21.8054 F6 bez FBS GAPDH Unknown 21.6744 G1 20 % FBS CCND1 Unknown 26.7425 G1 20 % FBS GAPDH_VIC Unknown 23.4736 G2 20 % FBS CCND1 Unknown 26.8596 G2 20 % FBS GAPDH_VIC Unknown 23.477 G3 20 % FBS CCND1 Unknown 26.7647 G3 20 % FBS GAPDH_VIC Unknown 23.36 G4 bez FBS CCND1 Unknown 27.5215 G4 bez FBS GAPDH_VIC Unknown 23.9195 G5 bez FBS CCND1 Unknown 27.5213 G5 bez FBS GAPDH_VIC Unknown 23.8862 G6 bez FBS CCND1 Unknown 27.6722 G6 bez FBS GAPDH_VIC Unknown 23.8818 G7 1 ul EtOH CCND1 Unknown 27.7152 G7 1 ul EtOH GAPDH_VIC Unknown 24.8987 G8 1 ul EtOH CCND1 Unknown 28.5036 G8 1 ul EtOH GAPDH_VIC Unknown 26.3069 G9 1 ul EtOH CCND1 Unknown 28.4017 G9 1 ul EtOH GAPDH_VIC Unknown 26.5516 G10 1 ul EtOH FOS Unknown Undetermined G10 1 ul EtOH GAPDH_VIC Unknown 26.7852 G11 1 ul EtOH FOS Unknown Undetermined G11 1 ul EtOH GAPDH_VIC Unknown 26.3738 G12 1 ul EtOH FOS Unknown Undetermined G12 1 ul EtOH GAPDH_VIC Unknown 26.4682 H1 20 % FBS FOS Unknown Undetermined H1 20 % FBS GAPDH_VIC Unknown 23.0579 H2 20 % FBS FOS Unknown Undetermined H2 20 % FBS GAPDH_VIC Unknown 23.4328 H3 20 % FBS FOS Unknown Undetermined H3 20 % FBS GAPDH_VIC Unknown 23.3075 H4 bez FBS FOS Unknown Undetermined H4 bez FBS GAPDH_VIC Unknown 23.8761 H5 bez FBS FOS Unknown Undetermined H5 bez FBS GAPDH_VIC Unknown 23.7271 H6 bez FBS FOS Unknown Undetermined H6 bez FBS GAPDH_VIC Unknown 23.8236 H7 2 ul EtOH CCND1 Unknown Undetermined H7 2 ul EtOH GAPDH_VIC Unknown Undetermined H8 2 ul EtOH CCND1 Unknown Undetermined H8 2 ul EtOH GAPDH_VIC Unknown Undetermined H9 2 ul EtOH CCND1 Unknown Undetermined H9 2 ul EtOH GAPDH_VIC Unknown Undetermined H10 2 ul EtOH FOS Unknown Undetermined H10 2 ul EtOH GAPDH_VIC Unknown Undetermined H11 2 ul EtOH FOS Unknown Undetermined H11 2 ul EtOH GAPDH_VIC Unknown Undetermined H12 2 ul EtOH FOS Unknown Undetermined H12 2 ul EtOH GAPDH_VIC Unknown Undetermined ##### Sheet/List 2 ##### Document Name: Skupina 2 Plate Type: Standard Curve Kalibrační křivky Relativní kvantifikace - duplex Vliv inhibitorů User: Student GAPDH 20 % FBS vs. no FBS CDK4 a GAPDH Document Information Operator: Administrator Run Date: Tuesday May 17 2016 15:16:59 Last Modified: Tuesday May 17 2016 16:41:44 Instrument Type: Applied Biosystems 7500 Real-Time PCR System Comments: SDS v1.4 Thermal Cycler Profile Stage Repetitions Temperature Time Ramp Rate 1 1 50.0 °C 2:00 100 2 1 95.0 °C 10:00 100 3 40 95.0 °C 0:15 100 60.0 °C 1:00 100 Standard 7500 Mode Data Collection : Stage 3 Step 2 PCR Volume: 25 µL Standard Curve Information Detector Name Slope Intercept R2 Number of Standards CDK4 -3.407207 40.353374 0.982914 16 CDK4 GAPDH_VIC -3.091311 38.382717 0.947460 35 20 % FBS vs. no FBS CCND2 a GAPDH CCND2 -3.016829 37.221371 0.967059 18 Well Sample Name Detector Task Ct Qty A1 CDK4 CDK4 Standard 22.665 150 A1 CDK4 GAPDH_VIC Standard 21.4199 150 A2 CDK4 CDK4 Standard 26.0398 15 A2 CDK4 GAPDH_VIC Standard 24.8129 15 A3 CDK4 CDK4 Standard 29.3405 1.5 A3 CDK4 GAPDH_VIC Standard 28.281 1.5 A4 CDK4 CDK4 Standard 33.3199 0.15 A4 CDK4 GAPDH_VIC Standard 32.0276 0.15 A5 CDK4 CDK4 Standard 37.3546 0.015 A5 CDK4 GAPDH_VIC Standard 35.8448 0.015 A6 CDK4 CDK4 Standard Undetermined 0.0015 A6 CDK4 GAPDH_VIC Standard 39.5989 0.0015 A7 CCND2 CCND2 Standard 20.9429 150 A7 CCND2 GAPDH_VIC Standard 22.3339 150 A8 CCND2 CCND2 Standard 24.2358 15 A8 CCND2 GAPDH_VIC Standard 25.4855 15 A9 CCND2 CCND2 Standard 27.8622 1.5 A9 CCND2 GAPDH_VIC Standard 29.1169 1.5 CCND2 A10 CCND2 CCND2 Standard 31.514 0.15 A10 CCND2 GAPDH_VIC Standard 32.4865 0.15 A11 CCND2 CCND2 Standard 34.8769 0.015 A11 CCND2 GAPDH_VIC Standard 34.751 0.015 A12 CCND2 CCND2 Standard 35.351 0.0015 Relativní kvantifikace - singlelex A12 CCND2 GAPDH_VIC Standard 34.7154 0.0015 B1 CDK4 CDK4 Standard 22.5856 150 20 % FBS vs. no FBS CDK4 B1 CDK4 GAPDH_VIC Standard 21.3815 150 B2 CDK4 CDK4 Standard 26.0939 15 B2 CDK4 GAPDH_VIC Standard 24.9349 15 B3 CDK4 CDK4 Standard 29.456 1.5 B3 CDK4 GAPDH_VIC Standard 28.4204 1.5 B4 CDK4 CDK4 Standard 33.3287 0.15 B4 CDK4 GAPDH_VIC Standard 32.2399 0.15 B5 CDK4 CDK4 Standard 37.4228 0.015 B5 CDK4 GAPDH_VIC Standard 36.1712 0.015 B6 CDK4 CDK4 Standard 37.5216 0.0015 B6 CDK4 GAPDH_VIC Standard Undetermined 0.0015 B7 CCND2 CCND2 Standard 21.0378 150 B7 CCND2 GAPDH_VIC Standard 22.3786 150 B8 CCND2 CCND2 Standard 24.3462 15 B8 CCND2 GAPDH_VIC Standard 25.7679 15 B9 CCND2 CCND2 Standard 27.9105 1.5 B9 CCND2 GAPDH_VIC Standard 29.2225 1.5 B10 CCND2 CCND2 Standard 31.3845 0.15 B10 CCND2 GAPDH_VIC Standard 32.7147 0.15 B11 CCND2 CCND2 Standard 35.1516 0.015 B11 CCND2 GAPDH_VIC Standard 34.3437 0.015 B12 CCND2 CCND2 Standard 35.223 0.0015 B12 CCND2 GAPDH_VIC Standard 34.679 0.0015 C1 CDK4 CDK4 Standard 22.4748 150 C1 CDK4 GAPDH_VIC Standard 21.3485 150 20 % FBS vs. no FBS CCND2 C2 CDK4 CDK4 Standard 26.0963 15 C2 CDK4 GAPDH_VIC Standard 25.0044 15 C3 CDK4 CDK4 Standard 29.3708 1.5 C3 CDK4 GAPDH_VIC Standard 28.4204 1.5 C4 CDK4 CDK4 Standard 32.9307 0.15 C4 CDK4 GAPDH_VIC Standard 32.2891 0.15 C5 CDK4 CDK4 Standard 36.729 0.015 C5 CDK4 GAPDH_VIC Standard 35.6028 0.015 C6 CDK4 CDK4 Standard Undetermined 0.0015 C6 CDK4 GAPDH_VIC Standard 39.811 0.0015 C7 CCND2 CCND2 Standard 21 150 C7 CCND2 GAPDH_VIC Standard 22.4595 150 C8 CCND2 CCND2 Standard 24.6767 15 C8 CCND2 GAPDH_VIC Standard 26.2081 15 C9 CCND2 CCND2 Standard 27.9771 1.5 C9 CCND2 GAPDH_VIC Standard 29.4567 1.5 C10 CCND2 CCND2 Standard 31.8735 0.15 C10 CCND2 GAPDH_VIC Standard 33.0923 0.15 C11 CCND2 CCND2 Standard 34.5189 0.015 C11 CCND2 GAPDH_VIC Standard 34.6886 0.015 C12 CCND2 CCND2 Standard 34.7826 0.0015 C12 CCND2 GAPDH_VIC Standard 34.6147 0.0015 D1 20% FBS CDK4 Unknown 23.2488 D2 20% FBS CDK4 Unknown 23.3523 D3 20% FBS CDK4 Unknown 23.4358 20 % FBS vs. no FBS GAPDH-FAM D4 bez FBS CDK4 Unknown 24.2256 D5 bez FBS CDK4 Unknown 24.1498 D6 bez FBS CDK4 Unknown 24.0953 D9 noRT CDK4 Unknown Undetermined D9 noRT GAPDH_VIC Unknown Undetermined D10 noRT CCND2 Unknown 36.0978 D10 noRT GAPDH_VIC Unknown 37.1215 D11 NK CDK4 Unknown Undetermined D11 NK GAPDH_VIC Unknown Undetermined D12 NK CCND2 Unknown 35.6778 D12 NK GAPDH_VIC Unknown 35.0815 E1 20% FBS CCND2 Unknown 21.4455 E2 20% FBS CCND2 Unknown 21.4271 E3 20% FBS CCND2 Unknown 21.4492 E4 bez FBS CCND2 Unknown 22.3928 E5 bez FBS CCND2 Unknown 22.7313 E6 bez FBS CCND2 Unknown 22.3888 E9 noRT CDK4 Unknown Undetermined E9 noRT GAPDH_VIC Unknown Undetermined E10 noRT CCND2 Unknown 35.2706 E10 noRT GAPDH_VIC Unknown 36.7261 E11 NK CDK4 Unknown Undetermined E11 NK GAPDH_VIC Unknown Undetermined E12 NK CCND2 Unknown 35.4738 E12 NK GAPDH_VIC Unknown 34.7101 F1 20% FBS GAPDH Unknown 20.5752 F2 20% FBS GAPDH Unknown 20.7305 F3 20% FBS GAPDH Unknown 20.5455 F4 bez FBS GAPDH Unknown 21.7196 F5 bez FBS GAPDH Unknown 21.531 F6 bez FBS GAPDH Unknown 21.4658 G1 20% FBS CDK4 Unknown 23.4688 G1 20% FBS GAPDH_VIC Unknown 23.0739 G2 20% FBS CDK4 Unknown 23.3199 G2 20% FBS GAPDH_VIC Unknown 22.9466 G3 20% FBS CDK4 Unknown 23.4103 G3 20% FBS GAPDH_VIC Unknown 22.9074 G4 bez FBS CDK4 Unknown 24.296 G4 bez FBS GAPDH_VIC Unknown 23.9272 G5 bez FBS CDK4 Unknown 24.2677 G5 bez FBS GAPDH_VIC Unknown 24.0036 G6 bez FBS CDK4 Unknown 24.3746 G6 bez FBS GAPDH_VIC Unknown 24.0781 G7 20% FBS 1 ul EtOH CDK4 Unknown 23.3002 G7 20% FBS 1 ul EtOH GAPDH_VIC Unknown 22.8017 G8 20% FBS 1 ul EtOH CDK4 Unknown 23.2661 G8 20% FBS 1 ul EtOH GAPDH_VIC Unknown 22.7362 G9 20% FBS 1 ul EtOH CDK4 Unknown 23.3593 G9 20% FBS 1 ul EtOH GAPDH_VIC Unknown 22.8891 G10 bez FBS 1 ul EtOH CDK4 Unknown 24.3977 G10 bez FBS 1 ul EtOH GAPDH_VIC Unknown 24.2269 G11 bez FBS 1 ul EtOH CDK4 Unknown 24.1755 G11 bez FBS 1 ul EtOH GAPDH_VIC Unknown 23.776 G12 bez FBS 1 ul EtOH CDK4 Unknown 24.3171 G12 bez FBS 1 ul EtOH GAPDH_VIC Unknown 23.8551 H1 20% FBS CCND2 Unknown 21.3678 H1 20% FBS GAPDH_VIC Unknown 22.9654 H2 20% FBS CCND2 Unknown 21.5359 H2 20% FBS GAPDH_VIC Unknown 23.1585 H3 20% FBS CCND2 Unknown 21.3107 H3 20% FBS GAPDH_VIC Unknown 22.6663 H4 bez FBS CCND2 Unknown 22.2496 H4 bez FBS GAPDH_VIC Unknown 23.7825 H5 bez FBS CCND2 Unknown 22.225 H5 bez FBS GAPDH_VIC Unknown 23.6861 H6 bez FBS CCND2 Unknown 22.2228 H6 bez FBS GAPDH_VIC Unknown 23.8479 H7 20% FBS 2 ul EtOH CCND2 Unknown 21.1505 H7 20% FBS 2 ul EtOH GAPDH_VIC Unknown 23.0562 H8 20% FBS 2 ul EtOH CCND2 Unknown 21.1193 H8 20% FBS 2 ul EtOH GAPDH_VIC Unknown 22.7694 H9 20% FBS 2 ul EtOH CCND2 Unknown 21.1879 H9 20% FBS 2 ul EtOH GAPDH_VIC Unknown 22.8954 H10 bez FBS 2 ul EtOH CCND2 Unknown 22.0949 H10 bez FBS 2 ul EtOH GAPDH_VIC Unknown 23.9774 H11 bez FBS 2 ul EtOH CCND2 Unknown 22.0481 H11 bez FBS 2 ul EtOH GAPDH_VIC Unknown 24.027 H12 bez FBS 2 ul EtOH CCND2 Unknown 21.9176 H12 bez FBS 2 ul EtOH GAPDH_VIC Unknown 23.8864 ##### Sheet/List 3 ##### Document Name: sk.3 Plate Type: Standard Curve Kalibrační křivky Relativní kvantifikace - duplex Vliv inhibitorů User: Student GAPDH 20 % FBS vs. no FBS CCND3 a GAPDH Document Information Operator: Administrator Run Date: Tuesday May 17 2016 13:30:12 Last Modified: Tuesday May 17 2016 15:13:43 Instrument Type: Applied Biosystems 7500 Real-Time PCR System Comments: SDS v1.4 Thermal Cycler Profile Stage Repetitions Temperature Time Ramp Rate 1 1 50.0 °C 2:00 100 2 1 95.0 °C 10:00 100 3 40 95.0 °C 0:15 100 60.0 °C 1:00 100 Standard 7500 Mode Data Collection : Stage 3 Step 2 PCR Volume: 25 µL Standard Curve Information Detector Name Slope Intercept R2 Number of Standards CCND3 -1.802252 41.689342 0.893183 13 GAPDH_VIC -1.049586 38.653770 0.601892 31 CCND3 20 % FBS vs. no FBS JUN a GAPDH Jun -1.200780 40.539257 0.787141 13 Well Sample Name Detector Task Ct Qty A1 CCND3 CCND3 Standard 31.6822 150 A1 CCND3 GAPDH_VIC Standard 31.2193 150 A2 CCND3 CCND3 Standard 34.2619 15 A2 CCND3 GAPDH_VIC Standard 32.531 15 A3 CCND3 CCND3 Standard 35.58 1.5 A3 CCND3 GAPDH_VIC Standard 33.9668 1.5 A4 CCND3 CCND3 Standard 38.0064 0.15 A4 CCND3 GAPDH_VIC Standard 36.0252 0.15 A5 CCND3 CCND3 Standard 38.1908 0.015 A5 CCND3 GAPDH_VIC Standard 37.1914 0.015 A6 CCND3 CCND3 Standard Undetermined 0.0015 A6 CCND3 GAPDH_VIC Standard 36.9226 0.0015 A7 JUN Jun Standard 33.0414 150 A7 JUN GAPDH_VIC Standard 34.2212 150 A8 JUN Jun Standard 35.3403 15 A8 JUN GAPDH_VIC Standard 34.5012 15 A9 JUN Jun Standard 37.6681 1.5 A9 JUN GAPDH_VIC Standard 35.796 1.5 A10 JUN Jun Standard 37.694 0.15 A10 JUN GAPDH_VIC Standard 37.4266 0.15 A11 JUN Jun Standard Undetermined 0.015 JUN Relativní kvantifikace - singlelex A11 JUN GAPDH_VIC Standard 39.7174 0.015 A12 JUN Jun Standard 39.2729 0.0015 20 % FBS vs. no FBS CCND3 A12 JUN GAPDH_VIC Standard Undetermined 0.0015 B1 CCND3 CCND3 Standard 31.7389 150 B1 CCND3 GAPDH_VIC Standard 30.8939 150 B2 CCND3 CCND3 Standard 35.5064 15 B2 CCND3 GAPDH_VIC Standard 33.2039 15 B3 CCND3 CCND3 Standard 35.4625 1.5 B3 CCND3 GAPDH_VIC Standard 33.6483 1.5 B4 CCND3 CCND3 Standard 38.7849 0.15 B4 CCND3 GAPDH_VIC Standard 36.2499 0.15 B5 CCND3 CCND3 Standard Undetermined 0.015 B5 CCND3 GAPDH_VIC Standard 37.2062 0.015 B6 CCND3 CCND3 Standard Undetermined 0.0015 B6 CCND3 GAPDH_VIC Standard 38.0236 0.0015 B7 JUN Jun Standard 33.1511 150 B7 JUN GAPDH_VIC Standard 35.7929 150 B8 JUN Jun Standard 36.4143 15 B8 JUN GAPDH_VIC Standard 35.4382 15 B9 JUN Jun Standard 37.1011 1.5 B9 JUN GAPDH_VIC Standard 36.4125 1.5 B10 JUN Jun Standard 37.8457 0.15 B10 JUN GAPDH_VIC Standard 38.1061 0.15 B11 JUN Jun Standard Undetermined 0.015 B11 JUN GAPDH_VIC Standard Undetermined 0.015 B12 JUN Jun Standard Undetermined 0.0015 20 % FBS vs. no FBS JUN B12 JUN GAPDH_VIC Standard Undetermined 0.0015 C1 CCND3 CCND3 Standard 31.6313 150 C1 CCND3 GAPDH_VIC Standard 31.3165 150 C2 CCND3 CCND3 Standard 35.2218 15 C2 CCND3 GAPDH_VIC Standard 33.1292 15 C3 CCND3 CCND3 Standard 36.561 1.5 C3 CCND3 GAPDH_VIC Standard 35.1207 1.5 C4 CCND3 CCND3 Standard 37.7107 0.15 C4 CCND3 GAPDH_VIC Standard 36.0381 0.15 C5 CCND3 CCND3 Standard Undetermined 0.015 C5 CCND3 GAPDH_VIC Standard 37.7974 0.015 C6 CCND3 CCND3 Standard Undetermined 0.0015 C6 CCND3 GAPDH_VIC Standard 37.3094 0.0015 C7 JUN Jun Standard 33.6049 150 C7 JUN GAPDH_VIC Standard 34.9433 150 C8 JUN Jun Standard 37.3182 15 C8 JUN GAPDH_VIC Standard 35.4424 15 C9 JUN Jun Standard 37.177 1.5 C9 JUN GAPDH_VIC Standard 36.72 1.5 C10 JUN Jun Standard 38.4114 0.15 C10 JUN GAPDH_VIC Standard 38.417 0.15 C11 JUN Jun Standard Undetermined 0.015 C11 JUN GAPDH_VIC Standard Undetermined 0.015 20 % FBS vs. no FBS GAPDH-FAM C12 JUN Jun Standard Undetermined 0.0015 C12 JUN GAPDH_VIC Standard Undetermined 0.0015 D1 20 % FBS CCND3 Unknown 26.7697 D1 20 % FBS GAPDH_VIC Unknown 23.0116 D2 20 % FBS CCND3 Unknown 27.0439 D2 20 % FBS GAPDH_VIC Unknown 23.0084 D3 20 % FBS CCND3 Unknown 27.5073 D3 20 % FBS GAPDH_VIC Unknown 24.6365 D4 bez FBS Jun Unknown 32.6336 D4 bez FBS GAPDH_VIC Unknown 21.042 D5 bez FBS Jun Unknown 32.372 D5 bez FBS GAPDH_VIC Unknown 21.3152 D6 bez FBS Jun Unknown 32.2304 D6 bez FBS GAPDH_VIC Unknown 21.1841 D9 noRT CCND3 Unknown Undetermined D9 noRT GAPDH_VIC Unknown 37.295 D10 noRT Jun Unknown 32.7535 D10 noRT GAPDH_VIC Unknown Undetermined D11 NK CCND3 Unknown Undetermined D11 NK GAPDH_VIC Unknown 37.9088 D12 NK Jun Unknown Undetermined D12 NK GAPDH_VIC Unknown Undetermined E1 20 % FBS CCND3 Unknown 27.1451 E1 20 % FBS GAPDH_VIC Unknown 23.44 E2 20 % FBS CCND3 Unknown 26.8919 E2 20 % FBS GAPDH_VIC Unknown 22.9904 E4 bez FBS Jun Unknown 32.7407 E4 bez FBS GAPDH_VIC Unknown 21.4883 E5 bez FBS Jun Unknown 33.422 E5 bez FBS GAPDH_VIC Unknown 22.2677 E9 noRT CCND3 Unknown 39.0647 E9 noRT GAPDH_VIC Unknown 37.518 E10 noRT Jun Unknown 32.7313 E10 noRT GAPDH_VIC Unknown Undetermined E11 NK CCND3 Unknown Undetermined E11 NK GAPDH_VIC Unknown 38.5889 E12 NK Jun Unknown Undetermined E12 NK GAPDH_VIC Unknown Undetermined F1 20 % FBS CCND3 Unknown 27.0163 F1 20 % FBS GAPDH_VIC Unknown 23.2268 F2 20 % FBS CCND3 Unknown 26.9301 F2 20 % FBS GAPDH_VIC Unknown 23.1804 F4 bez FBS Jun Unknown 33.2588 F4 bez FBS GAPDH_VIC Unknown 21.5904 F5 bez FBS Jun Unknown 33.5547 F5 bez FBS GAPDH_VIC Unknown 22.2412 F7 20 % FBS CCND3 Unknown 27.0737 F8 20 % FBS CCND3 Unknown 27.0051 F9 20 % FBS CCND3 Unknown 26.8199 F10 20 % FBS Jun Unknown 33.7437 F11 20 % FBS Jun Unknown 33.9659 F12 20 % FBS Jun Unknown 33.7599 G1 EtOH 2 ul CCND3 Unknown Undetermined G1 EtOH 2 ul GAPDH_VIC Unknown Undetermined G2 EtOH 2 ul CCND3 Unknown Undetermined G2 EtOH 2 ul GAPDH_VIC Unknown 38.1705 G3 EtOH 2 ul CCND3 Unknown Undetermined G3 EtOH 2 ul GAPDH_VIC Unknown Undetermined G4 EtOH 2 ul Jun Unknown Undetermined G4 EtOH 2 ul GAPDH_VIC Unknown Undetermined G5 EtOH 2 ul Jun Unknown Undetermined G5 EtOH 2 ul GAPDH_VIC Unknown Undetermined G6 EtOH 2 ul Jun Unknown Undetermined G6 EtOH 2 ul GAPDH_VIC Unknown Undetermined G7 bez FBS CCND3 Unknown 25.0844 G8 bez FBS CCND3 Unknown 25.5685 G9 bez FBS CCND3 Unknown 25.8599 G10 bez FBS Jun Unknown 32.7212 G11 bez FBS Jun Unknown 32.0021 G12 bez FBS Jun Unknown 31.3697 H1 EtOH 1 ul CCND3 Unknown 25.9335 H1 EtOH 1 ul GAPDH_VIC Unknown 22.4354 H2 EtOH 1 ul CCND3 Unknown 26.1301 H2 EtOH 1 ul GAPDH_VIC Unknown 22.3416 H3 EtOH 1 ul CCND3 Unknown 26.1328 H3 EtOH 1 ul GAPDH_VIC Unknown 23.2082 H4 EtOH 1 ul Jun Unknown Undetermined H4 EtOH 1 ul GAPDH_VIC Unknown 26.0033 H5 EtOH 1 ul Jun Unknown Undetermined H5 EtOH 1 ul GAPDH_VIC Unknown 25.7224 H6 EtOH 1 ul Jun Unknown Undetermined H6 EtOH 1 ul GAPDH_VIC Unknown 23.7337 H7 20 % FBS GAPDH Unknown 21.0077 H8 20 % FBS GAPDH Unknown 20.6605 H9 20 % FBS GAPDH Unknown 20.3281 H10 bez FBS GAPDH Unknown 19.9743 H11 bez FBS GAPDH Unknown 19.3953 H12 bez FBS GAPDH Unknown 18.4614 ##### Sheet/List 4 ##### Document Name: skupina 3 Plate Type: Standard Curve User: Student Document Information Operator: Administrator Run Date: Tuesday May 19 2015 15:28:19 Last Modified: Tuesday May 19 2015 16:57:56 Instrument Type: Applied Biosystems 7500 Real-Time PCR System Comments: SDS v1.4 Thermal Cycler Profile Stage Repetitions Temperature Time Ramp Rate 1 1 50.0 °C 2:00 100 2 1 95.0 °C 10:00 100 3 40 95.0 °C 0:15 100 60.0 °C 1:00 100 4 (Dissociation) 1 95.0 °C 0:15 Auto 60.0 °C 1:00 Auto 95.0 °C 0:15 Auto 60.0 °C 0:15 Auto Standard 7500 Mode Data Collection : Stage 3 Step 2 PCR Volume: 25 µL GAPDH FBS vs. no FBS GAPDH a CCND3 Well Sample Name Detector Task Ct StdDev Ct Qty A1 GAPDH_VIC Standard 19.4175 150 A1 CCND3 Standard 23.4349 150 A2 GAPDH_VIC Standard 21.6803 15 A2 CCND3 Standard 25.8999 15 A3 GAPDH_VIC Standard 25.0957 1.5 A3 CCND3 Standard 29.2836 1.5 A4 GAPDH_VIC Standard 28.4504 0.15 A4 CCND3 Standard 32.4464 0.15 A5 GAPDH_VIC Standard 31.8103 0.015 A5 CCND3 Standard 35.3045 0.015 A6 GAPDH_VIC Standard 35.3691 0.0015 A6 CCND3 Standard Undetermined 0.0015 A7 NK GAPDH_VIC Unknown 39.5253 A7 NK CCND3 Unknown Undetermined A8 noRT GAPDH_VIC Unknown Undetermined A8 noRT CCND3 Unknown Undetermined A9 noRT GAPDH_VIC Unknown 39.3293 A9 noRT CCND3 Unknown Undetermined A10 bez FBS GAPDH_VIC Unknown 22.2828 A10 bez FBS CCND3 Unknown 26.3634 A11 bez FBS GAPDH_VIC Unknown 22.3949 A11 bez FBS CCND3 Unknown 26.3719 A12 bez FBS GAPDH_VIC Unknown 23.0142 A12 bez FBS CCND3 Unknown 26.2957 CCND3 B1 GAPDH_VIC Standard 18.6189 150 FBS vs. no FBS GAPDH a JunD B1 CCND3 Standard 22.6283 150 B2 GAPDH_VIC Standard 21.8138 15 B2 CCND3 Standard 26.0068 15 B3 GAPDH_VIC Standard 25.1659 1.5 B3 CCND3 Standard 29.3774 1.5 B4 GAPDH_VIC Standard 28.6282 0.15 B4 CCND3 Standard 32.7353 0.15 B5 GAPDH_VIC Standard 32.064 0.015 B5 CCND3 Standard 36.8365 0.015 B6 GAPDH_VIC Standard 35.2233 0.0015 B6 CCND3 Standard 39.7392 0.0015 B7 NK GAPDH_VIC Unknown Undetermined B7 NK JunD Unknown Undetermined B8 noRT GAPDH_VIC Unknown Undetermined B8 noRT JunD Unknown Undetermined B9 noRT GAPDH_VIC Unknown Undetermined B9 noRT JunD Unknown Undetermined B10 bez FBS GAPDH_VIC Unknown 22.259 B10 bez FBS JunD Unknown 26.3573 B11 bez FBS GAPDH_VIC Unknown 22.3422 B11 bez FBS JunD Unknown 26.3632 B12 bez FBS GAPDH_VIC Unknown 22.0592 B12 bez FBS JunD Unknown 26.3279 C1 GAPDH_VIC Standard 18.7298 150 C1 CCND3 Standard 22.7195 150 JunD C2 GAPDH_VIC Standard 21.8245 15 C2 CCND3 Standard 25.9791 15 C3 GAPDH_VIC Standard 24.7397 1.5 C3 CCND3 Standard 29.0607 1.5 Vliv inhibitorů C4 GAPDH_VIC Standard 28.6941 0.15 C4 CCND3 Standard 32.5152 0.15 C5 GAPDH_VIC Standard 31.4033 0.015 C5 CCND3 Standard 35.5964 0.015 C6 GAPDH_VIC Standard 35.2178 0.0015 C6 CCND3 Standard Undetermined 0.0015 C10 20 % FBS GAPDH_VIC Unknown 22.2913 C10 20 % FBS CCND3 Unknown 26.4279 C11 20 % FBS GAPDH_VIC Unknown 22.2198 C11 20 % FBS CCND3 Unknown 26.4324 C12 20 % FBS GAPDH_VIC Unknown 22.1536 C12 20 % FBS CCND3 Unknown 26.2809 D1 GAPDH_VIC Standard 18.8789 150 D1 JunD Standard 24.5394 150 D2 GAPDH_VIC Standard 21.8041 15 D2 JunD Standard 26.1271 15 D3 GAPDH_VIC Standard 25.1906 1.5 D3 JunD Standard 28.7587 1.5 D4 GAPDH_VIC Standard 28.6436 0.15 D4 JunD Standard 31.8381 0.15 D5 GAPDH_VIC Standard 32.1099 0.015 D5 JunD Standard 35.0348 0.015 D6 GAPDH_VIC Standard 34.9345 0.0015 D6 JunD Standard 37.8332 0.0015 D10 20 % FBS GAPDH_VIC Unknown 22.4276 D10 20 % FBS JunD Unknown 26.2645 D11 20 % FBS GAPDH_VIC Unknown 22.3302 D11 20 % FBS JunD Unknown 26.0897 D12 20 % FBS GAPDH_VIC Unknown 22.185 D12 20 % FBS JunD Unknown 27.5725 E1 GAPDH_VIC Standard 18.8997 150 E1 JunD Standard 24.6273 150 E2 GAPDH_VIC Standard 21.9112 15 E2 JunD Standard 25.7467 15 E3 GAPDH_VIC Standard 25.2805 1.5 E3 JunD Standard 28.993 1.5 E4 GAPDH_VIC Standard 28.6066 0.15 E4 JunD Standard 31.1467 0.15 E5 GAPDH_VIC Standard 32.1377 0.015 E5 JunD Standard 34.094 0.015 E6 GAPDH_VIC Standard 35.5773 0.0015 E6 JunD Standard 37.705 0.0015 E10 EtOH 1 ul GAPDH_VIC Unknown 29.5014 E10 EtOH 1 ul CCND3 Unknown 28.439 E11 EtOH 1 ul GAPDH_VIC Unknown Undetermined E11 EtOH 1 ul CCND3 Unknown Undetermined E12 EtOH 1 ul GAPDH_VIC Unknown Undetermined E12 EtOH 1 ul CCND3 Unknown Undetermined F1 GAPDH_VIC Standard 18.8186 150 F1 JunD Standard 24.312 150 F2 GAPDH_VIC Standard 21.8637 15 F2 JunD Standard 26.2044 15 F3 GAPDH_VIC Standard 25.0908 1.5 F3 JunD Standard 28.8951 1.5 F4 GAPDH_VIC Standard 28.7065 0.15 F4 JunD Standard 31.8502 0.15 F5 GAPDH_VIC Standard 32.008 0.015 F5 JunD Standard 36 0.015 F6 GAPDH_VIC Standard 35.536 0.0015 F6 JunD Standard 39.2465 0.0015 F10 EtOH 1 ul GAPDH_VIC Unknown Undetermined F10 EtOH 1 ul JunD Unknown Undetermined F11 EtOH 1 ul GAPDH_VIC Unknown Undetermined F11 EtOH 1 ul JunD Unknown Undetermined F12 EtOH 1 ul GAPDH_VIC Unknown Undetermined F12 EtOH 1 ul JunD Unknown Undetermined G10 EtOH 2 ul GAPDH_VIC Unknown Undetermined G10 EtOH 2 ul CCND3 Unknown Undetermined G11 EtOH 2 ul GAPDH_VIC Unknown Undetermined G11 EtOH 2 ul CCND3 Unknown Undetermined G12 EtOH 2 ul GAPDH_VIC Unknown Undetermined G12 EtOH 2 ul CCND3 Unknown Undetermined H10 EtOH 2 ul GAPDH_VIC Unknown Undetermined H10 EtOH 2 ul JunD Unknown Undetermined H11 EtOH 2 ul GAPDH_VIC Unknown Undetermined H11 EtOH 2 ul JunD Unknown Undetermined H12 EtOH 2 ul GAPDH_VIC Unknown Undetermined H12 EtOH 2 ul JunD Unknown Undetermined