• • Amoebozoa – Archamoebae (Rhizopoda) - Entamoeba Trophozoites of E. histolytica with ingested erythrocytes (red blood cells) stained with trichrome. Entaboeba histolytica Trofozoiti a cysty améb Trofozoiti a cysty améb • Life cycle of Entamoeba histolytica • Entamoeba histolytica - patogenita Volně žijící améby: 1) Excavata – Heterolobosea – Naegleria 2) Amebozoa – Lobosa - Acanthamoeba Obecná charakteristika Volně žijící améby • Life cycle of Naegleria fowleri • Naegleria fowleri - trofozoit Naegleria fowleri - trofozoit Naegleria fowleri - cysta Transformace Neagleria fowleri Naegleria gruberi Acanthamoeba sp. Balamuthia mandrillaris • Acanthamoeba, Indirect Immunofluorescence Balamuthia, Indirect Immunofluorescence •Nepřímá imunofluorescence Inflammed eye. •GAE Acanthamoeba castellanii • Life cycle of Acanthamoeba spp. • Magnified 250X, this photomicrograph revealed the presence of Acanthamoeba sp. parasitic microorganisms, which were found in a specimen of human H&E-stained adrenal gland tissue. Acanthamoeba sp. – cysty v mozku • Acanthamoeba cyst in tissue, H&E Acanthamoeba cyst in tissue, H&E • •Barvení: hematoxylin – eosin, H&E • Acanthamoeba trophozoite in tissue, H&E Acanthamoeba trophozoites in tissue, H&E Acanthamoeba sp. – cysty ve tkáni •Barvení: hematoxylin – eosin, H&E Acathamoeba castellanii - trofozoit Acanthamoeba polyphaga - cysty Balamuthia mandrillaris • B. mandrillaris trophozoite in tissue, H&E B. mandrillaris trophozoite in tissue, H&E •Balamunthia mandrillaris – trofozoiti v mozku • Life cycle of Balamuthia mandrillaris • • •Balamuthia mandrillaris Balamuthia mandrillaris • B. mandrillaris cyst in tissue, H&E B. mandrillaris cyst in tissue, H&E •Balamuthia mandrillaris – cysty v mozkové tkáni, barveno H & E Balamuthia mandrillaris • B. mandrillaris cyst in tissue, H&E B. mandrillaris cyst in tissue, H&E •Balamuthia mandrillaris – cysty v mozkové tkáni, barveno H & E •