System A					System B					System C	
											
Column					Column					Column	
"Length, mm"	150				"Length, mm"	150				"Length, mm"	150
"ID, mm"	2.1				"ID, mm"	2.1				"ID, mm"	2.1
"dp, mm"	1.6				"dp, mm"	1.6				"dp, mm"	1.6
"VM, ml*"	0.53	0.31			"VM, ml*"	0.48	0.31			"VM, ml*"	0.48
											
Dwell volume		0.525			Dwell volume					Dwell volume	
"VD, ml"	0.18				"VD, ml"	0.18				"VD, ml"	0.18
											
											
Injection volume					Injection volume					Injection volume	
"Vi, ul"	5				"Vi, ul"	5.00				"Vi, ul"	5.00
											
Mobile phase					Mobile phase			 		Mobile phase	
"Flow-rate, ml/min"	0.35				"Flow-rate, ml/min"	0.35				"Flow-rate, ml/min"	2.00
											
Pressure					Pressure					Pressure	
"Pressure, MPa"	5.4				"Pressure, MPa"	5.4				"Pressure, MPa"	30.9
											
											
"Equilibrium time, min"	15				"Equilibrium time, min"	14.8				"Equilibrium time, min"	2.6
											
											
											
"Time, min"	"B, %"				"Time, min"	"B, %"				"Time, min"	"B, %"
0	5				0	5				0	5
5	5				4.57	5				0.80	5
15	95	25.71428571			13.71	95	28.125			2.40	95
20	95				18.29	95				3.20	95
21	5				19.20	5				3.36	5
30	5				27.43	5				4.80	5
											
"Run time, min"	20				"Run time, min"	18.29				"Run time, min"	3.20
											
											
											
											
											
											
											
											
											
											
											
											
											
											
											
											
											
	System A					System B					System B
VM/VG	0.0750					0.0750					0.0750
"Analysis time, min"	35					33.13					5.80
Analysis/hour	1.7					1.8					10.3
ml/Analysis	7					6.4					6.4
											
											
* - 60% of colume volume as a first approximation