BRIEF INSTRUCTIONS

1) Listen to the teacher's explanation, resp. go through presentations (study materials) that the
teacher has prepared for this lesson (gametogenesis and oogenesis).

2) Follow the instructions provide below,  prepare your protocol (with theoretical and practical
part) on their basis. Prepare the theoretical part to a reasonable extent so that it serves for a
better understanding of the practical part (can be processed on a PC).

3) If drawings are required in the practical part, handwritten drawings are meant (drawing by hand
= effective form of learning J).

4) Rule: drawing of one microscopic slide = half A4; always provide the information about the
magnification at which you observe a tissue/organ; label your drawing in detail;

5) Hand over your protocol to the teacher in a next week's lesson.

6) Questions and consultations: helanej@sci.muni.cz


THEORY


Here the student prepares a theoretical introduction to the topic.




























PRACTICAL PART

*     Exercise 1: Mammalian ovary - a permanent slide

Determine what types of follicles are in the photo. Try to find these structures in the permanent
slide that is available to you during the class. Do you find any other structures in the ovary?
Draw the ovary in the protocol and describe the microscopic anatomy in detail.

File:Ovary histology 005.jpg




















*     Exercise 2: Folikulogenesis

Based on the previous information provided by the teacher and the diagram below, try to  explain
the following terms:

002f.gif (510×376) 1) atresia

2) granulosa cells

3) antral follicle

4) ovulation











*     Exercise 3: Comparison of human and avian egg cells

Using teaching materials provided by the teacher and suitable internet resources, try to compare
avian and human egg cells (focus e.g on the amount of yolk, size ..., origin and development, etc.)



Birds (Aves)

Humans (Homo)

1.

produced (in most species) only by the left ovary

alternating asymmetric production in both ovaries

2.



3.



4.



5.



6.













*     Exercise 4: Mammalian  testes - a permanent slide

a) Study the permanent slide - draw and describe the microscopic anatomy the mammal testes.



















b)  What is the blood-testis barrier? Which cells form the barrier? Try to find them in the
observed organ and label the nucleus in your drawing of the testes. What is the function of the
barrier? What are the possible consequences of its damage?












*     Exercise 5: Sperm smears of various animal species - permanent slides

 Study the microscopic slides - sperm smears of various animal species. Draw the observed sperm and
describe the structure of the observed  sperm in detail.


obr.


























*     Exercise 6: Fertilization - permanent slides

Study the microscopic slides with fertilized sea urchin eggs. What type of eggs can we observe in
sea urchins? Be aware of the difference between the egg size and the sperm size. Draw and describe
your observation.


















CONCLUSION


(It isvoluntary to formulate a conclusion in the case of this lesson)









LITERATURE


Erickson, G. Global library of women's medicine. 2008. ISSN: 1756-2228. DOI 10.3843/GLOWM.10289

Hodge R. Developmental Biology: from a Cell to an Organism. 1st edition, 2010. ISBN
978-0-8160-6683-4.

https://cit.vfu.cz/opvk2014/?title=ukoly-rozmnozovani_a_vyvoj&lang=cz.

https://www.sciencedirect.com/science/article/pii/B9780123725684000033.

Mäkelä JA., Toppari J. Spermatogenesis. In: Simoni M., Huhtaniemi I. (eds) Endocrinology of the
Testis and Male Reproduction. 2017. Endocrinology. Springer, Cham.
https://doi.org/10.1007/978-3-319-44441-3_13https://embryology.med.unsw.edu.au/embryology/index.php
?title=File:Ovary_histology_005.jpg

Skinner, B., Johnson, E. Nuclear morphologies: their diversity and functional relevance.
Chromosoma. 2017, 126. 1-18. 10.1007/s00412-016-0614-5