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Structural Virology
Lecture 10
Pavel Plevka
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Lecture on 12.12. will take place in A35
- excursion to virology, cryo-EM and X-ray lab
- demonstration of structural-biology approaches used in virology
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Emerging viruses
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Bunyaviridae
Segmented -ssRNA linear
genome, L segment is
between 6.8 and 12 kb, M
segment between 3.2 and
4.9 kb and S segment
between 1 and 3 kb.
Encodes for four to six
proteins.
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Paramyxoviridae
-ssRNA ±15kB
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Newcastle disease virus
matrix protein
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Newcastle disease virus – matrix protein
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Newcastle disease virus
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Paramyxoviridae - rubulavirus
Mumps
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Rubella – capsid protein
structure
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Flaviviridae
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Flaviviridae - genome
Flaviviruses
 Tick-borne encephalitis
 West Nile
 Yellow fever
 Dengue (strains 1-4)
Mukhopadhyay et al. Science, 2003
I Yu et al. Science 2008
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I Yu et al. Science 2008L Li et al. Science 2008
Dengue2 prR201A maturation mutant
Furin cleavage
site
prR201A particles with “double” symmetry
Correlation with model in
orientation 1
Correlation with model in
orientation 2
Combination of tomography and single
particle data
Structure of Dengue 2 maturation
intermediate
Correlation with immature
model
Correlation with low-pH
immature model
Relative orientations of immature and low-pH
immature domains
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Poxviridae
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Coronaviridae
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Coronaviridae
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Learning outcomes
• know the term ‘emerging virus’
• discuss examples of viruses that have recently
appeared in new host species
• discuss examples of viruses that have recently
appeared in new parts of the world
• discuss examples of new viruses
• discuss examples of re-emerging viruses
• assess measures that can be taken to prevent
and contain outbreaks of infectious disease
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Viruses and cancer
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Papillomaviruses
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Human Polyomaviruses
Merkel cell carcinoma
Mouse polyomavirus
Progressive Multifocal
Encephalopathy (JCV)
MCV BKV JCV KIV WUV
Inf. pop. (%) 42 82 39 55 69
SI to Mouse PyV 52 51 51 26 25
SI to SV40 48 81 76 26 27
Feng et al. Science, 2008; Koralnik. A Neur. 2006; Stehle et al. Nature,
Li et al. Nature, 2003
Human Polyomaviruses
Stehle et al. Nature, 1994 Liddington et al. Nature, 1991
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Learning outcomes
• outline the characteristics of viruses that are
associated with cancers;
• evaluate the evidence for association of viruses
with some cancers;
• discuss possible mechanisms for virus induction
of cancer;
• suggest how virus-induced cancers may be pre-
vented.
John B. Carter and Venetia A. Saunders
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Figures
Chapter 24
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Bacteriophages
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