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1. Introductory lecture
Organization of the course
1. Introductory lecture
Organization of the course
Bi7430 Molecular Biotechnology
In t r o d u c t i o n o f c o u r s e
C o n t e n t o f c o u r s e a n d p r a c t i c a l c l a s s
L e c t u r i n g a n d e v a l u a t i o n
R e c o m m e n d e d l i t e r a t u r e
B i o t e c h n o l o g y a t MU ( E xc u r s i o n i n L L )
Outline
Introduction of Course
EXT ENSIVE MULT IDISCIPLI NARIT Y
PREREQUISI TES:
basic knowledge of microbiology, molecular biology, biochemistry,
immunology and genetics
COURSE FOCUSE:
the specif ic aspect s of modern biotechnology
examples of up to date applications in industry,
agriculture, pharmacy, biomedicine
and env ironmental protection
the role of modern biotechnology in sustainabl e living
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Sustainability
concept of sustainabili ty
with the aim to promote a necessary “… dev elopment that meets the
needs of the present without compromising the ability of f uture
generations to meet their own needs”
* W o r l d C o m m i s s i o n o n E n v i r o n m e n t a n d D e v e l o p m e n t , 1 9 8 7
Sustainability
reduce env ironmental impact
reduce consumpti on of resources
(e.g., raw materials, energy, air, water)
reduce waste production
increase the recycling of waste
increase use of renewable materi al s
(e.g., biomass)
Biotechnology
KEY T ECHNOLOGY of 21st century
ENVIRONMENTAL ASPECTS
natural processes (bioprocesses)
sustainabl e and resource eff icient
ECONOMICAL ASPECTS
1/3 of worldwide production deriv ed f rom bioprocesses
biotechnol ogy market 300 billion EUR*
* R o u t e t o t h e K n o w l e d g e - B a s e d B i o e c o n o m y , 2 0 0 7
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Content of course
2. Introduction to Molecular Biotechnolog y
3. Protein Engineering
4. Metabolic Engineering I.
5. Metabolic Engineering II.
6. Molecular Biotechnolog y in Industry
7. Molecular Biotechnolog y in Agricultur e
8. Molecular Biotechnolog y in Medicine I.
9. Molecular Biotechnolog y in Medicine II.
10. Molecular Biotechnolog y in Medicine III.
11. Environment al molecular biotechnolog y
METHODOLOGICAL
LECTURES
TECHNOLOGICAL
LECTURES
Content of practicals
BLOK A (VRI)
1. Preparat ion and transformat ion of liposoms
2. Analysi s of liposoms (e.g., DLS, TEM)
BLOK B (UKB, INBIT)
3. Preparat ion of recombinant E. coli
4. Cultivat ion of recombinant E. coli
5. Purificat ion of recombinant protein
6. Preparat ion of biosensor
7. Biocatalyt ic preparat ion of pharmaceutic al precursor
Lecturers
Doc. RNDr. Zbyněk Prokop, Ph.D. (UČO 23696)
Loschmidt Laboratories, leader of research team
co-founder and CEO of Enantis – 1s t biotech spin-off at MU
protein and metabolic engineer ing
biotechnologic al applicat ions
Mgr. Pavel Dvořák (UČO 151419)
Loschmidt Laboratories, research specialist
protein and metabolic engineer ing
molecular biology and biotechnolog y
RNDr. Jaroslav Turánek, CSc. (UČO 28301)
Veterinar y Research Institut e
head of Dept. of Pharmacology and Immunotherap y
immunotherap y, recombinant vaccines
nanotechnology and drug deliver y systems
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Instructors
Loschmidt laboratories, MU
Lukáš Chrást, M.Sc. (UČO 269981)
Hana Moskalíkov á, M.Sc. (UČO 113231)
Koen Beerens, Ph.D. (UČO 233293)
Enantis, Ltd.
Šárka Bidmanov á, Ph.D. (UČO 77580)
Veronika Štěpánkov á, Ph.D. (UČO 106723)
Veterinary Research Institute
PharmDr. Josef Mašek, Ph.D.
Ing. Štěpán Koudelka, Ph.D.
MVDr. Pav el Kulich, Ph.D.
RNDr. Jana Plockov á
Instructions
bring printed copy of the slides as handouts f or notes
Instructions
bring printed copy of the slides as handouts f or notes
f ind all materials including printed v ersion
of the slides at http://is.mun i. cz/
be on time, come at least 5 min bef ore the lecture opening
if any problems with the lecture, please, contact lecturers
be active and participate in discussion s
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Lecturing system
powerpoint slides as well as recommended literature in English
lecturing, discussions and examination in Czech
2 hrs per week
lecture part I. (45 min)
BREAK (5-10 min)
lecture part II. (45 min)
Evaluation
written essay
Czech or English, one A4 page, 1.5 spaced, TNR 12
Topic 1: „The most interest ing/p rom ising molecular biotechnology“
Topic 2: „Safety and ethical aspects of molecular biotechnology“
Topic 3: „GMOs: Future or emerging apocalyp se? “
Introduction – main body – discussion – conclusions – references
progress written tests during the lecturing period
at the beginning of lecture 4., 6., 8., 10. and 12. (duration 10 min)
each 10-12 questions from previou s topics (cumulative)
final written test during the examination period
50 questions from entire content of the course / 1.5 hour
Recommended literature
M. W ink (Ed.) 2011: An Introduction to Molecular Biotechnology:
Fundamentals, Methods and Applicat ion s, 2n d Edition, W illey-B lackw el l
B. R. Glick, J. J. Pasternak, C. L. Patten 2011: Molecular Biotechnology:
Principles and Applicat ions of Recombinant DNA,
4t h Edition, ASM Press
J. M. W alker, R. Rapley 2009: Molecular biology and biotechnology,
5t h Edition, RSC Publish ing
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R E S E A R C H A N D D E V E L O P M E N T O F B I O T E C H N O L O G I C A L A P P L I C AT I O N SR E S E A R C H A N D D E V E L O P M E N T O F B I O T E C H N O L O G I C A L A P P L I C AT I O N S
B I O S E N S I N G
B I O C ATA LY S I S
B I O D E G R A D AT I O N
Loschmidt Laboratories, MU
Biocatalysis
1 P r o k o p , Z . , D a m b o r s k y , J . , N a g a t a , Y . , J a n s s e n , D . B . 2 0 0 4 . W O 2 0 0 6 0 7 9 2 9 5
2 P r o k o p , Z . , S a t o , Y . , B r e z o v s k y , J . , e t a l . 2 0 1 0 . A n g e w . C h e m . I n t . E d . 4 9 : 6 1 1 1 – 6 1 1 5
D r u g s
F e r o m o n e s
B r a d y r h i s o b i u m
j a p o n i c u m
Biodegradation
3 P r o k o p , Z . , D a m b o r s k y , J . , O p l u s t i l , F . , J e s e n s k a , A . , N a g a t a , Y . 2 0 0 5 . W O 2 0 0 6 1 2 8 3 9 0
4 P r o k o p , Z . , O p l u s t i l , F . , D e F r a n k , J . , D a m b o r s k y , J . 2 0 0 6 . B i o t e c h . J . 1 : 1 3 7 0 - 1 3 8 0
S p h i n g o b i u m
j a p o n i c u m
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Biosensing
5 B i d m a n o v a , e t a l . 2 0 1 0 . A n a l y t i c a l a n d B i o a n a l y t i c a l C h e m i s t r y 3 9 8 : 1 8 9 1 – 1 8 9 8
6 B i d m a n o v a , e t a l . 2 0 1 0 . C h e m i c k e L i s t y 1 0 4 : 3 0 2 - 3 0 8
Mol Biotechnology at MU
R e s e a r c h
D e v e l o p m e n t
A p l i c a t i o n s
E d u c a t i o n