Report LightCycler® 480 Software . iiiiiiiiiii ^Roche) blaZ, sk. II, 9.12.2014 Experiment Creation Date 12/9/2014 2:51:11 PM Last Modified Date 12/9/2014 4:11:03 PM Operator LMDM Owner LMDM Start Time 12/9/2014 3:02:58 PM End Time 12/9/2014 4:07:01 PM Run State Completed Software Version LCS480 1.5.1.62 Macro Macro Owner Macro Status Templates blaZ kvantifikace cviko, blaZ sk. II, 9.12.2014 Plate ID 03413001 Test ID Lot ID Color Comp ID Run Notes dhghrhjdrjj. u,ldr,r,.ryr.ru.;.;d Programs Program Name stage 1 Cycles 1 Analysis Mode None Target (°C) Acquisition Mode Hold (hh:mm:ss) Ramp Rate (°C/s) Acquisitions (per °C) Sec Target (°C) Step size (°C) Step Delay (cycles) 50 None 00:02:00 4.40 0 0 0 Program Name stage 2 Cycles 1 Analysis Mode None Target (°C) Acquisition Mode Hold (hh:mm:ss) Ramp Rate (°C/s) Acquisitions (per °C) Sec Target (°C) Step size (°C) Step Delay (cycles) 95 None 00:05:00 4.40 0 0 0 Program Name stage 3 Cycles 40 Analysis Mode Quantification Target (°C) Acquisition Mode Hold (hh:mm:ss) Ramp Rate (°C/s) Acquisitions (per °C) Sec Target (°C) Step size (°C) Step Delay (cycles) 95 None 00:00:10 4.40 0 0 0 60 Single 00:00:30 2.20 0 0 0 Program Name stage 4 Cycles 1 Analysis Mode Melting Curves Target (°C) Acquisition Mode Hold (hh:mm:ss) Ramp Rate (°C/s) Acquisitions (per °C) Sec Target (°C) Step size (°C) Step Delay (cycles) 95 None 00:00:15 4.40 0 0 0 60 None 00:01:00 2.20 0 0 0 blaZ, sk. II, 9.12.2014 12/9/2014 Page 1 of 5 - Target (°C) Acquisition Mode Hold (hh:mm:ss) Ramp Rate (°C/s) Acquisitions (per °C) Sec Target (°C) Step size (°C) Step Delay (cycles) 95 Continuous 0.11 5 0 0 0 Program Name cooling Cycles 1 Analysis Mode None Target (°C) Acquisition Mode Hold (hh:mm:ss) Ramp Rate (°C/s) Acquisitions (per °C) Sec Target (°C) Step size (°C) Step Delay (cycles) 40 None 00:01:00 2.20 0 0 0 Samples Sample Count 96 Subset All Samples Pos Name ID Repl. Of Sample Notes A1 pi. DNA JB A1 A2 pi. DNA JB A1 A3 pi. DNA JB A1 A4 pi. DNA JB A4 A5 pi. DNA JB A4 A6 pi. DNA JB A4 A7 pi. DNA JB A7 A8 pi. DNA JB A7 A9 pi. DNA JB A7 A10 pi. DNA JB A10 A11 pi. DNA JB A10 A12 pi. DNA JB A10 B1 pi. DNA JB B1 B2 pi. DNA JB B1 B3 pi. DNA JB B1 B4 pi. DNA JB B4 B5 pi. DNA JB B4 B6 pi. DNA JB B4 B7 pi. DNA JB B7 B8 pi. DNA JB B7 B9 pi. DNA JB B7 B10 0 B11 0 B12 0 C1 629 ehr. ner. C1 C2 629 ehr. ner. C1 C3 629 ehr. ner. C1 C4 629 chr. red. C4 C5 629 chr. red. C4 C6 629 chr. red. C4 blaZ, sk. II, 9.12.2014 12/9/2014 Page 2 of 5 Samples Sample Count] 96 Subset All Samples Pos Name ID Repl. Of Sample Notes C7 759 chr. ner. C7 C8 759 chr. ner. C7 C9 759 chr. ner. C7 C10 759 chr. red. C10 C11 759 chr. red. C10 C12 759 chr. red. C10 D1 759 pi. ner D1 D2 759 pi. ner D1 D3 759 pi. ner D1 D4 759 pi. red. D4 D5 759 pi. red. D4 D6 759 pi. red. D4 D7 kontrola D7 D8 kontrola D7 D9 kontrola D7 D10 Sample 46 D11 Sample 47 D12 Sample 48 E1 Sample 49 E2 Sample 50 E3 Sample 51 E4 Sample 52 E5 Sample 53 E6 Sample 54 E7 Sample 55 E8 Sample 56 E9 Sample 57 E10 Sample 58 E11 Sample 59 E12 Sample 60 F1 Sample 61 F2 Sample 62 F3 Sample 63 F4 Sample 64 F5 Sample 65 F6 Sample 66 F7 Sample 67 F8 Sample 68 F9 Sample 69 blaZ, sk. II, 9.12.2014 12/9/2014 Page 3 of 5 Samples Sample Count] 96 Subset All Samples Pos Name ID Repl. Of Sample Notes F10 Sample 70 F11 Sample 71 F12 Sample 72 G1 Sample 73 G2 Sample 74 G3 Sample 75 G4 Sample 76 G5 Sample 77 G6 Sample 78 G7 Sample 79 G8 Sample 80 G9 Sample 81 G10 Sample 82 G11 Sample 83 G12 Sample 84 H1 Sample 85 H2 Sample 86 H3 Sample 87 H4 Sample 88 H5 Sample 89 H6 Sample 90 H7 Sample 91 H8 Sample 92 H9 Sample 93 H10 Sample 94 H11 Sample 95 H12 Sample 96 Revision History Reusion Date User Reason Abs Quant/2nd Derivative Max for All Samples (Abs Quant/2nd Derivative Max) Settings Channel 465-510 Color Compensation Off Program stage 3 Units Mode High Confidence blaZ, sk. II, 9.12.2014 12/9/2014 Page 4 of 5 Subset Name All Samples blaZ, sk. II, 9.12.2014 12/9/2014 Page 5 of 5