Varianta 1

5´301 TGAAAATGGTCGTTTTATCGAGGACGAAGTTGTTTGTCGTTTCCGTGGTAATAACACAGT

                               NdeI >>>>>>>>>>>>>>>>>>>>

                                M  V  V  C……

                  5´ TATCGAGCATATGGTTGTTTGTCGTTTCCGTGGTA 3´

                     ||||||| |    ||||||||||||||||||||||

3´    ACTTTTACCAGCAAAATAGCTCCTGCTTCAACAAACAGCAAAGGCACCATTATTGTGTCA


Varianta 2

5´301 TGAAAATGGTCGTTTTATCGAGGACGAAGTTGTTTGTCGTTTCCGTGGTAATAACACAGT

                               NdeI >>>>>>>>>>>>>>>>>>>>

                                   M  V  C ……

                  5´    CGAGGACCATATGGTTTGTCGTTTCCGTGGTA 3´

                        ||||||| |  | |||||||||||||||||||

3´    ACTTTTACCAGCAAAATAGCTCCTGCTTCAACAAACAGCAAAGGCACCATTATTGTGTCA



  421 AACAGCATGTATCCCATTCTTAGAAAACGATGACTCAAACCGTGCGTTAATGGGTGCAAA



  481 CATGCAACGACAAGCAGTACCATTGATGAACCCAGAAGCACCATTTGTTGGTACAGGTAT

                              ||||||||||||||||||||   |  ||

                              CTACTTGGGTCTTCGTGGTACCTAGGCA

                          3´  <<<<<<<<<<<<<<<<<<<< 5´

      BamHI 5´G^GATCC 3´


   5´ACGGATCCATGGTGCTTCTGGGTTCATC 3´