1 2 3 4 5 6 7 8 9 10 11 12 In case of splincerette, the primer is of the same sequence as the top strand and therefore it is unable to act as a primer until the complement of this strand has been synthesized (from the insert-specific primer at the right-hand side). 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40