A new strategy of reagents labeling (NPs, QDs) used in immunoassay with LA-ICP-MS

Michaela Tvrdonova ^1, Marketa Vaculovicova^2,3, Andrea Moravanska^3, Tereza Vaneckova^3 and Tomas
                                          Vaculovic ^1,4

^1Department of Chemistry, Faculty of Science, Masaryk University in Brno, Kotlarska 2, 611 37
Brno, ^2 Brno Univ Technol, Cent European Inst Technol, Purkynova 123, CZ-61200 Brno, Czech
Republic, ^3Mendel Univ Brno, Dept Chem & Biochem, Zemedelska 1, CZ-61300 Brno, Czech Republic,
^4CEITEC Masaryk University in Brno  Kamenice 5, 625 00 Brno


Laser ablation with inductively coupled plasma is still more used in life science as biology and
biomedicine and the utilization of metals and proteins determination simultaneously is also growing
up. ^2, 3 We have developed a new strategy of labeling of antibody (it can specific binds to
proteins) by nanoparticles and quantum dots which is composed of thousands of atoms and thus
increases the sensitivity enormously and of course decreases the limit of detection, compare to
lanthanoids labeling. The ability of successfully tagged antibodies bound to antigen (protein) was
proved by dot blot on membrane imaged by LA-ICP-MS.












1.         S. Kanje, A. J. Herrmann, S. Hober, and L. Mueller, Next generation of labeling reagents
for quantitative and multiplexing immunoassays by the use of LA-ICP-MS. Analyst, 141, (2016):6374.

2.         L. Waentig, N. Jakubowski, S. Hardt, C. Scheler, P. H. Roos, and M. W. Linscheid,
Comparison of different chelates for lanthanide labeling of antibodies and application in a Western
blot immunoassay combined with detection by laser ablation (LA-)ICP-MS. Journal of Analytical
Atomic Spectrometry, 27, (2012):1311.