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Structural	Virology	
Lecture	7	
Pavel	Plevka	
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Rhabdoviridae	
Virions	are	bullet	shaped	180	nm	long	and	75	nm	wide.	
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Vesicular	stomaIIs	virus	
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Rabies	virus	
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ALachment	and	cell	entry	
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Virion	organizaIon	
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Genome	organizaIon	and	sequenIal	transcripIon	
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SequenIal	transcripIon	
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NegaIve-stranded	RNA	virus	polymerase	stuLering	
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NegaIve-stranded	RNA	virus	replicaIon	
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TranslaIon	and	post-translaIonal	modificaIons	
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Budding	
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Viruses	with	minus	or	ambisense	RNA	genomes	
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Arenaviridae	(Lassa	fever)	
Fever,	facial	swelling,	muscle	faIgue,	
conjuncIviIs	and	mucosal	bleeding.	
GastrointesInal	tract	
•  Nausea	
•  VomiIng	(bloody)	
•  Diarrhea	(bloody)	
•  Stomach	ache	
•  ConsIpaIon	
•  Dysphagia	(difficulty	swallowing)	
•  HepaIIs	
Cardiovascular	system	
•  PericardiIs	
•  Hypertension	
•  Hypotension	
•  Tachycardia	
Respiratory	tract	
•  Cough	
•  Chest	pain	
•  Dyspnoea	
•  PharyngiIs	
•  PleuriIs	
Nervous	system	
•  EncephaliIs	
•  MeningiIs	
•  Hearing	deficit	
•  Seizures	
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																																															Ebola	virus	
Symptoms	
•  Fever	
•  Severe	headache	
•  Muscle	pain	
•  Weakness	
•  FaIgue	
•  Diarrhea	
•  VomiIng	
•  Abdominal	(stomach)	
pain	
•  Unexplained	hemorrhage	
(bleeding	or	bruising)	
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Ambisense	virus	translaIon	
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Learning	outcomes	
•  discuss	the	importance	of	rabies	virus,	vesicular	stomaIIs	
virus	and	other	minus-strand	RNA	viruses	
•  describe	the	rhabdovirus	virion	
•  outline	the	main	characterisIcs	of	the	rhabdovirus	
genome	
•  discuss	the	replicaIon	cycle	of	rhabdoviruses	
•  explain	the	term	‘ambisense	genome’	
•  discuss	the	development	of	reverse	geneIcs	procedures	
for	minus-strand	RNA	viruses	
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Orthomyxoviridae	-	Influenza	
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																								HemaggluInin	
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Membrane	fusion	
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Cap	snatching	
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AlternaIve	splicing	
Leaky	scanning	
Ribosomal	frameshij	
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AnIgenic	drij	x	anIgenic	shij	
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Learning	outcomes	
•  Describe	structure	of	Influenza	A	virion	
•  Describe	the	Influenza	A	genome	
•  Describe	Influenza	A	replicaIon	cycle	
•  Explain	anIgenic	drij	and	shij	
•  Explain	membrane	fusion	mechanism	
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Retroviridae	
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Reverse	transcriptases	
Murine	leukemia	virus	(MLV)			
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Reverse	transcriptase	
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Learning	outcomes	
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