Horizontálny prenos génov pomocou Gene transfer agents
BÍ7311, 27. 9. 2018
INTRODUCTION
What is GTA
Virion maturation
Structural Cluster
Multilocus "genome"
100 nm
Small phage-like particle
GTA
No GTA
Frequency of KanR transfer
2,5*10
4,6*10
Efficient gene exchange system
(a)
|D|. aos/D/ - 0.3 /d|. 0.1 /d/ - 0.5 f
CtrA CtrA-P Gtal Y
Y
Y
Y
Rep
Y
yy x*
ft
Y Y«
Y
Y
(b)
6 37* 8}9M
11 3 12
13 )M4
15
/
rcc01681
rcc01698
RcGTA gene cluster
1 kb
Current Opinion in Microbiology
(A ^1 T
Prophage or phage
Expression and i replication ICjl
lOlQ
o
(a)
1. RcGTA production and DNA packaging
DNA transduction
2. Lytic release
C
e
to the environment " , x\
■ j
, M./ 3. Adsorption by head spikes and capsular receptor
Polysaccharide capsule
Cytoplasmic membrane
4. DNA ejection to periplasm
DNA transformation
5. DNA transport to cytoplasm and homologous recombination
(b)
Bacterial regulators
CtrA (response regulato GtaR/l (quorum sensing)
/ \
Donor cells
RcGTA particle production Maturation (DNA, spikes) Lytic release
Recipient cells
/^Capsular receptor
Polysaccharide synthesis
Natural competence ComM ComF ComEC DprA
Current Opinion in Microbiology
RESULTS
Attachment of the GTA
INTRODUCTION
Why to study GTA
Marine ecology