PE/Cy7 anti-human CD24 Antibody
Catalog# / Size 311119 / 25 tests
311120 / 100 tests
Clone ML5 (See other available formats)
Workshop V CD24.5
Other Names Ly-52, Heat Stable Antigen (HSA), Nectadrin, BA-1
Isotype Mouse IgG2a, κ
Description CD24 is a 35-45 kD glycosylphosphatidylinositol (GPI)-linked protein also known as heat
stable antigen (HSA), BA-1, Ly-52, and nectadrin. It is expressed on the surface of B cells (but
not plasma cells), granulocytes, follicular dendritic cells, and epithelial cells. CD24 may play
a role in the regulation of B-cell proliferation and maturation. CD24 crosslinking induces a
Ca flux in mature B cells. CD24 has been shown to interact with CD62P (P-selectin).2+
Product Details
Reactivity Human, Cross-Reactivity: Chimpanzee
Antibody Type Monoclonal
Host Species Mouse
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin
USA).
Preparation The antibody was purified by affinity chromatography, and conjugated with PE/Cy7 under optimal
conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Concentration Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup
tool if you have a lot number.)
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from
prolonged exposure to light. Do not freeze.
Application FC - Quality tested
Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric
analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100
µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes Additional reported applications (for the relevant formats) include: immunofluorescence microscopy .3
Application References
(PubMed link indicates
BioLegend citation)
1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. Oxford
University Press. New York.
2. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
3. Yang GP, et al. 1999. Nucleic Acids Research 27:1517. (IF)
4. Kristiansen G, et al. 2003. Clin. Cancer Res. 9:4906. (FC)
Product Citations
1. Ordonez M, et al. 2012. Hum Mol Genet. 2.715972222. PubMed
2. Hamilton K, et al. 2013. Carcinogenesis. 34:2647. PubMed
3. Ames E, et al. 2015. J Immunol. 195: 4010 - 4019. PubMed
4. Bagnara D, et al. 2015. J Immunol. 195: 3716 - 3724. PubMed
5. Mohiuddin M, et al. 2016. Nat Commun. 7: 11138. PubMed
6. Joosten S, et al. 2016. PLoS Pathog. 12: 1005687. PubMed
7. Deshmukh A, et al. 2017. Sci Rep. 7:2256. PubMed
RRID AB_2072734 (BioLegend Cat. No. 311119)
AB_2259843 (BioLegend Cat. No. 311120)
Antigen Details
Version: 2 Revision Date: 08/09/2013
Structure GPI-linked glycoprotein, 35-45 kD
Distribution B cells, granulocytes, epithelial cells
Function B cell proliferation and differentiation
Ligand Receptor CD62P (P-Selectin)
Antigen References 1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V. Oxford University Press. New York.
Gene ID 100133941
Related Protocols
Cell Surface Flow Cytometry Staining Protocol
FITC anti-human CD24, PE anti-human CD24, Purified anti-human CD24, Alexa Fluor® 488 anti-human CD24, Alexa Fluor® 647 antihuman
CD24, PerCP/Cyanine5.5 anti-human CD24, APC anti-human CD24, Brilliant Violet 421™ anti-human CD24, Brilliant Violet
605™ anti-human CD24, PerCP anti-human CD24, Brilliant Violet 510™ anti-human CD24, Purified anti-human CD24 (MaxPar®
Ready), Biotin anti-human CD24, APC/Cy7 anti-human CD24, PE/Dazzle™ 594 anti-human CD24, Brilliant Violet 711™ anti-human
CD24, TotalSeq™-A0180 anti-human CD24, APC/Fire™ 750 anti-human CD24
Other Formats
Product Data
Human peripheral blood lymphocytes
stained with ML-5 PE/Cy7
For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other
violations that may occur with the use of our products.
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Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or
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