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LightCycler® 480 Software
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20191022SAU
Experiment
Creation Date	10/22/2019 1:16:08 PM	Last Modified Date	10/23/2019 8:01:06 AM
Operator	LMDM	Owner	LMDM
Start Time	10/22/2019 2:32:38 PM	End Time	10/22/2019 3:49:07 PM
Run State	Completed	Software Version	LCS480 1.5.1.62
Macro		Macro Owner	
Macro Status			
Templates	abs. kvantifikace mecA cvika, gene blaZ absolute quantification SYBER GREEN	Plate ID	07409364
Test ID		Lot ID	
Color Comp ID			
Run Notes	dhghrhjdrjj. u,ldr,r,.ryr.ru.;.;d		
Programs
Pr	ogram Name	stage 1							
	Cycles	1	Analysis Mode | None						
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	50	None		00:02:00	4.40		0	0	0
Program Name		stage 2							
Cycles		1	Analysis Mode | None						
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	95	None		00:05:00	4.40		°		°
Program Name		stage 3							
Cycles		50	Analysis Mode		Quantification				
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	95	None		00:00:10	4.40		0	0	0
	60	Single		00:00:30	2.20		0	0	0
Program Name		stage 4							
Cycles		1	Analysis Mode		Melting Curves				
	Target (°C)	Acquisition Mode		Hold (hh:mm:ss)	Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	95	None		00:00:15	4.40		0	0	0
	60	None		00:01:00	2.20		0	0	0
20191022SAU
10/23/2019
Page 1 of 8
	Target (°C)	Acquisition Mode	Hold (hh:mm:ss)	Ramp Rate CC/s)	Acquisitions (per'C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	95	Continuous		0.11	5	0	0	0
Program Name		cooling						
Cycles		1              Analysis Mode		None				
	Target (°C)	Acquisition Mode	Hold (hh:mm:ss)	- Ramp Rate (°C/s)	Acquisitions (per°C)	Sec Target (°C)	Step size (°C)	Step Delay (cycles)
	40	None	00:01:00	2.20		0	0	0
Samples
Sample Count
96
Subset All Samples
Pos	Name	ID	Repl. Of	Sample Notes
A1	Sample 1			
A2	Sample 2			
A3	Sample 3			
A4	Sample 4			
A5	Sample 5			
A6	Sample 6			
A7	Sample 7			
A8	Sample 8			
A9	Sample 9			
A10	Sample 10			
A11	Sample 11			
A12	Sample 12			
B1	Sample 13			
B2	Sample 14			
B3	Sample 15			
B4	Sample 16			
B5	Sample 17			
B6	Sample 18			
B7	Sample 19			
B8	Sample 20			
B9	Sample 21			
B10	Sample 22			
B11	Sample 23			
B12	Sample 24			
C1	Sample 25			
C2	Sample 26			
C3	Sample 27			
C4	Sample 28			
C5	Sample 29			
C6	Sample 30			
20191022SAU
10/23/2019
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Samples
Sample Count
96
Subset All Samples
Pos	Name	ID	Repl. Of	Sample Notes
C7	Sample 31			
C8	Sample 32			
C9	Sample 33			
C10	Sample 34			
C11	Sample 35			
C12	Sample 36			
D1	Sample 37			
D2	Sample 38			
D3	Sample 39			
D4	Sample 40			
D5	Sample 41			
D6	Sample 42			
D7	Sample 43			
D8	Sample 44			
D9	Sample 45			
D10	Sample 46			
D11	Sample 47			
D12	Sample 48			
E1	Sample 49			
E2	Sample 50			
E3	Sample 51			
E4	Sample 52			
E5	Sample 53			
E6	Sample 54			
E7	Sample 55			
E8	Sample 56			
E9	Sample 57			
E10	Sample 58			
E11	Sample 59			
E12	Sample 60			
F1	Sample 61			
F2	Sample 62			
F3	Sample 63			
F4	Sample 64			
F5	Sample 65			
F6	Sample 66			
F7	Sample 67			
F8	Sample 68			
F9	Sample 69			
20191022SAU
10/23/2019
Page 3 of 8
Samples
Sample Count
96
Subset
All Samples
Pos	Name	ID	Repl. Of	Sample Notes
F10	Sample 70			
F11	Sample 71			
F12	Sample 72			
G1	Sample 73			
G2	Sample 74			
G3	Sample 75			
G4	Sample 76			
G5	Sample 77			
G6	Sample 78			
G7	Sample 79			
G8	Sample 80			
G9	Sample 81			
G10	Sample 82			
G11	Sample 83			
G12	Sample 84			
H1	Sample 85			
H2	Sample 86			
H3	Sample 87			
H4	Sample 88			
H5	Sample 89			
H6	Sample 90			
H7	Sample 91			
H8	Sample 92			
H9	Sample 93			
H10	Sample 94			
H11	Sample 95			
H12	Sample 96			
Revision History
Revision	Date	User	Reason
			
Abs Quant/2nd Derivative Max for All Samples (Abs Quant/2nd Derivative Max) Settings
Channel	465-510		
Color Compensation	Off		
Program	stage 3	Units	
Mode	High Confidence		
20191022SAU
10/23/2019
Page 4 of 8
Subset Name All Samples
Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
0	A1	RN4220 SAU	Standard	25.61	2.89E4	2.70E4	
0	A2	RN4220 SAU	Standard	25.85	2.53E4	2.70E4	
0	A3	RN4220 SAU	Standard	25.57	2.96E4	2.70E4	
0	A4	RN4220 SAU	Standard	31.28	1.28E3	2.70E3	
0	A5	RN4220 SAU	Standard	30.11	2.43E3	2.70E3	
0	A6	RN4220 SAU	Standard	30.19	2.33E3	2.70E3	
0	A7	RN4220 SAU	Standard	33.53	3.71 E2	2.70E2	
0	A8	RN4220 SAU	Standard	34.09	2.73E2	2.70E2	
0	A9	RN4220 SAU	Standard	33.96	2.93E2	2.70E2	
0	A10	RN4220 SAU	Standard	37.57	2.48E1	2.70E1	
0	A11	RN4220 SAU	Standard	38.33	1.29E1	2.70E1	
0	A12	RN4220 SAU	Standard	39.29	5.42E0	2.70E1	
0	B1	RN4220 SAU	Standard	39.51	4.39E0	2.70E0	
□	B2	RN4220 SAU	Standard			2.70E0	
□	B3	RN4220 SAU	Standard			2.70E0	
□	B4	RN4220 SAU	Standard			2.70E-1	
□	B5	RN4220 SAU	Standard			2.70E-1	
□	B6	RN4220 SAU	Standard			2.70E-1	
□	B7	RN4220 SAU	Standard			2.70E-2	
0	B8	RN4220 SAU	Standard	43.04	9.21 E-2	2.70E-2	
0	B9	RN4220 SAU	Standard	45.00	7.24E-3	2.70E-2	>
0	B10	C1	Unknown				
0	B11	C1	Unknown				
0	B12	C1	Unknown				
0	C1	T5	Unknown	26.82	1.48E4		
0	C2	T5	Unknown	26.54	1.73E4		
0	C3	T5	Unknown	26.33	1.95E4		
0	C4	T1	Unknown	24.79	4.55E4		E
0	C5	T1	Unknown	25.29	3.45E4		E
0	C6	T1	Unknown	25.80	2.60E4		
0	C7	COL	Unknown	24.48	5.39E4		E
0	C8	COL	Unknown	24.67	4.86E4		E
0	C9	COL	Unknown	25.72	2.73E4		E
0	C10	neg. kontrol.	Negative Control	20.47	4.89E5		
0	C11	neg. kontrol.	Negative Control	20.42	5.01 E5		
> - Late Cp call (last five cycles) has higher uncertainty, E - Extrapolated concentration in standard curve, ? - Detector Call uncertain
20191022SAU
10/23/2019
Page 5 of 8
Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
EI	C12	neg. kontrol.	Negative Control	20.27	5.45E5		
EI	D1	Sample 37	Unknown				
0	D2	Sample 38	Unknown				
0	D3	Sample 39	Unknown				
0	D4	Sample 40	Unknown				
0	D5	Sample 41	Unknown				
0	D6	Sample 42	Unknown				
0	D7	Sample 43	Unknown				
0	D8	Sample 44	Unknown	26.00	2.34E4		?
0	D9	Sample 45	Unknown				
0	D10	Sample 46	Unknown				
0	D11	Sample 47	Unknown				
0	D12	Sample 48	Unknown				
0	E1	Sample 49	Unknown	36.17	7.24E1		
0	E2	Sample 50	Unknown				
0	E3	Sample 51	Unknown				
0	E4	Sample 52	Unknown				
0	E5	Sample 53	Unknown				
0	E6	Sample 54	Unknown				
0	E7	Sample 55	Unknown				
0	E8	Sample 56	Unknown				
0	E9	Sample 57	Unknown				
0	E10	Sample 58	Unknown				
0	E11	Sample 59	Unknown				
0	E12	Sample 60	Unknown	32.04	8.42E2		
0	F1	Sample 61	Unknown				
0	F2	Sample 62	Unknown				
0	F3	Sample 63	Unknown	30.57	1.89E3		
0	F4	Sample 64	Unknown				
0	F5	Sample 65	Unknown				
0	F6	Sample 66	Unknown				
0	F7	Sample 67	Unknown				
0	F8	Sample 68	Unknown				
0	F9	Sample 69	Unknown				
0	F10	Sample 70	Unknown				
0	F11	Sample 71	Unknown				
0	F12	Sample 72_	Unknown				
> - Late Cp call (last five cycles) has higher uncertainty, E - Extrapolated concentration in standard curve, ? - Detector Call uncertain
20191022SAU
10/23/2019
Page 6 of 8
Results
Inc	Pos	Name	Type	CP	Concentration	Standard	Status
0	G1	Sample 73	Unknown				
0	G2	Sample 74	Unknown	30.54	1.92E3		?
0	G3	Sample 75	Unknown				
0	G4	Sample 76	Unknown				
0	G5	Sample 77	Unknown				
0	G6	Sample 78	Unknown				
0	G7	Sample 79	Unknown				
0	G8	Sample 80	Unknown				
0	G9	Sample 81	Unknown				
0	G10	Sample 82	Unknown				
0	G11	Sample 83	Unknown				
0	G12	Sample 84	Unknown				
0	H1	Sample 85	Unknown				
0	H2	Sample 86	Unknown				
0	H3	Sample 87	Unknown				
0	H4	Sample 88	Unknown				
0	H5	Sample 89	Unknown				
0	H6	Sample 90	Unknown	25.00	4.04E4		?, E
0	H7	Sample 91	Unknown	24.85	4.39E4		E
0	H8	Sample 92	Unknown	23.56	8.94E4		?, E
0	H9	Sample 93	Unknown	33.83	3.14E2		
0	H10	Sample 94	Unknown				
0	H11	Sample 95	Unknown				
0	H12	Sample 96	Unknown				
> - Late Cp call (last five cycles) has higher uncertainty, E - Extrapolated concentration in standard curve, ? - Detector Call uncertain
20191022SAU
10/23/2019
Page 7 of 8
Amplification Curves
10
15
20
25 30 Cycles
35
40
45
50
Standard Curve
Error: 0.0652 Efficiency: 1.734 Slope: -4.185 Ylntercept: 44.28 Link: 293.0
Std. curve Samples
0 1 2
Log Concentration
Analysis Notes
20191022SAU
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