Product Data Sheet
Catalog # / Size: 2603120 / 100 µg
2603115 / 25 µg
Clone: RTK4530
Isotype: Rat IgG2b, κ
Immunogen: Trinitrophenol + KLH
Preparation: The immunoglobulin was purified by
affinity chromatography, and
conjugated with APC/Cy7 under optimal
conditions. The solution is free of
unconjugated APC/Cy7 and
unconjugated antibody.
Formulation: Phosphate-buffered solution, pH 7.2,
containing 0.09% sodium azide.
Concentration: 0.2
Applications:
Applications: Flow Cytometry
Recommended
Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining
with flow cytometric analysis as negative control. Use at concentrations
comparable to those of the specific antibody of interest.
Application
Notes:
The RTK4530 immunoglobulin is useful as an isotype-matched control (for the
relevant formats) for Western blotting, immunoprecipitation,
immunohistochemistry, functional assay, and immunofluorescence microscopy.
The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered)
is recommended for functional assays (Cat. No. 400622) as negative control. For
in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified
antibody (Cat. No. 400644) with a lower endotoxin limit than standard LEAF™
purified antibodies (Endotoxin <0.01 EU/microg).
Application
References:
1. Cervantes-Barragan L, et al. 2007. Blood 109:1131.
2. Zeiser R, et al. 2007. Blood 109:2225.
3. Sasaki K, et al. 2008. J. Immunol. 181:104. PubMed
4. Duan J, et al. 2008. P. Natl. Acad. Sci. USA 105:5183. PubMed
5. Yi H, et al. 2009. Blood 113:5819. PubMed
6. Schafeer JS, et al. 2010. J. Leukocyte Biol. 87:301. PubMed
Description: The isotype of RTK4530 immunoglobulin is rat IgG2b, κ. This antibody was chosen
as an isotype control after screening on a variety of resting, activated, live, and
fixed mouse, rat and human tissues.
APC/Cy7 Rat IgG2b, κ Isotype Ctrl
For research use only. Not for diagnostic use. Not for resale. Sony Biotechnology Inc. will not be held responsible
for patent infringement or other violations that may occur with the use of our products.
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