Product Data Sheet
Catalog # / Size: 1115140 / 100 µg
1115135 / 25 µg
Clone: IM7
Isotype: Rat IgG2b, κ
Immunogen: Dexamethasone-induced myeloid
leukemia M1 cells
Reactivity: Human
Preparation: The antibody was purified by affinity
chromatography, and conjugated with
APC/Cy7 under optimal conditions. The
solution is free of unconjugated APC/Cy7
and unconjugated antibody.
Formulation: Phosphate-buffered solution, pH 7.2,
containing 0.09% sodium azide.
Concentration: 0.2
C57BL/6 splenocytes stained with
IM7 APC/Cy7
Applications:
Applications: Flow Cytometry
Recommended
Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining
with flow cytometric analysis. For flow cytometric staining, the suggested use of
this reagent is ≤1.0 microg per million cells in 100 microL volume. It is
recommended that the reagent be titrated for optimal performance for each
application.
Application
Notes:
Clone IM7 has been reported to recognize an epitope common to alloantigens and
all isoforms of CD4417,18 that is located between amino acids 145 and 18620.
Additional reported applications (for the relevant formats) include:
immunohistochemistry of acetone-fixed frozen sections and formalin-fixed
paraffin-embedded sections6,7, complement-mediated cytotoxicity1,
immunoprecipitation1,3, and in vivo inhibition of DTH4,5. The LEAF™ purified
antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for
functional assays (Cat. No. 103014). For highly sensitive assays, we recommend
Ultra-LEAF™ purified antibody (Cat. No. 103046) with a lower endotoxin limit than
standard LEAF™ purified antibodies (Endotoxin <0.01 EU/microg).
Application
References:
1. Trowbridge IS, et al. 1982. Immunogenetics 15:299. (ICFC, IP, CMCD)
2. Katoh S, et al. 1994. J. Immunol. 153:3440. (ELISA)
3. Budd RC, et al. 1987. J. Immunol. 138:3120. (IP)
4. Camp RL, et al. 1993. J. Exp. Med. 178:497. (Block)
5. Weiss JM, et al. 1997. J. Cell Biol. 137:1137. (Block)
6. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) PubMed
7. Cuff CA, et al. 2001. J. Clin. Invest. 108:1031. (IHC)
8. Lee JW, et al. 2006. Nature Immunol. 8:181.
9. Zhang N, et al. 2005. J. Immunol. 174:6967. PubMed
10. Huabiao C, et al. 2005. J. Immunol. 175:591. PubMed
11. Gui J, et al. 2007. Int. Immunol. 19:1201. PubMed
12. Wang XY, et al. 2008. Blood 111:2436. PubMed
13. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
14. Yamazaki J, et al. 2009. Blood PubMed
15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
16. Chen YW, et al. 2010. Mol. Cancer Ther. 9:2879. PubMed
APC/Cy7 anti-mouse/human CD44
For research use only. Not for diagnostic use. Not for resale. Sony Biotechnology Inc. will not be held responsible
for patent infringement or other violations that may occur with the use of our products.
Sony Biotechnology Inc. 1730 North First Street, San Jose, CA 95112 www.sonybiotechnology.com
17. Zheng Z, et al. 1995. J. Cell. Biol. 130:485.
18. Wiranowska M, et al. 2010. Int. J. Cancer 127:532.
19. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547.
PubMed
20. Sandmaier BM, et al. 1998. Blood 91:3494.
21. Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed
22. Young KH, et al. 2014. Cancer Immunol Res. 2:1011. PubMed
Description: CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH.
It is expressed on all leukocytes, endothelial cells, hepatocytes, and
mesenchymal cells. As B and T cells become activated or progress to the memory
stage, CD44 expression increases from low or mid levels to high levels. Thus,
CD44 has been reported to be a valuable marker for memory cell subsets. High
CD44 expression on Treg cells has been associated with potent suppressive
function via high production of IL-10. CD44 is an adhesion molecule involved in
leukocyte attachment to and rolling on endothelial cells, homing to peripheral
lymphoid organs and to the sites of inflammation, and leukocyte aggregation.
Antigen
References:
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al
For research use only. Not for diagnostic use. Not for resale. Sony Biotechnology Inc. will not be held responsible
for patent infringement or other violations that may occur with the use of our products.
Sony Biotechnology Inc. 1730 North First Street, San Jose, CA 95112 www.sonybiotechnology.com