G0-G1
53,2%
G2-M
26,9%
S
0,6%
cell cycle
96,6%
VL1-A :: VL1 PO-PRO-A
x
VL1-W :: VL1 PO-PRO-W
Compact population
86,8%
FSC-A :: FSC-A
x
SSC-A :: SSC-A
Single Cells
59,1%
FSC-A :: FSC-A
x
FSC-H :: FSC-H
Live cells
56,0%
BL3-A :: BL3 LD Red-A
x
SSC-A :: SSC-A
0 3.0M 6.0M 9.0M
cell cycle PO-PRO
10
0
10
1
10
2
10
3
10
4
10
5
10
6
EdUclickiTAF488
PO-PRO test DU145 Martina J ctrl izo.fcs
cell cycle
27,8%
171,0
G0-G1
64,0%
G2-M
21,1%
S
0,0%
cell cycle
98,3%
VL1-A :: VL1 PO-PRO-A
x
VL1-W :: VL1 PO-PRO-W
Compact population
91,3%
FSC-A :: FSC-A
x
SSC-A :: SSC-A
Single Cells
66,1%
FSC-A :: FSC-A
x
FSC-H :: FSC-H
Live cells
57,1%
BL3-A :: BL3 LD Red-A
x
SSC-A :: SSC-A
0 3.0M 6.0M 9.0M
cell cycle PO-PRO
10
0
10
1
10
2
10
3
10
4
10
5
10
6
EdUclickiTAF488
PO-PRO test DU145 Martina J ctrl mln izo.fcs
cell cycle
33,9%
114,0
G0-G1
30,9%
G2-M
8,9%
S
55,6%
cell cycle
99,2%
VL1-A :: VL1 PO-PRO-A
x
VL1-W :: VL1 PO-PRO-W
Compact population
95,0%
FSC-A :: FSC-A
x
SSC-A :: SSC-A
Single Cells
58,9%
FSC-A :: FSC-A
x
FSC-H :: FSC-H
Live cells
85,3%
BL3-A :: BL3 LD Red-A
x
SSC-A :: SSC-A
0 3.0M 6.0M 9.0M
cell cycle PO-PRO
10
0
10
1
10
2
10
3
10
4
10
5
10
6
EdUclickiTAF488
PO-PRO test DU145 Martina J ctrl mln spec.fcs
cell cycle
47,4%
4687,0
G0-G1
38,4%
G2-M
15,1%
S
40,8%
cell cycle
99,2%
VL1-A :: VL1 PO-PRO-A
x
VL1-W :: VL1 PO-PRO-W
Compact population
97,2%
FSC-A :: FSC-A
x
SSC-A :: SSC-A
Single Cells
49,4%
FSC-A :: FSC-A
x
FSC-H :: FSC-H
Live cells
91,4%
BL3-A :: BL3 LD Red-A
x
SSC-A :: SSC-A
0 3.0M 6.0M 9.0M
cell cycle PO-PRO
10
0
10
1
10
2
10
3
10
4
10
5
10
6
EdUclickiTAF488
PO-PRO test DU145 Martina J ctrl spec.fcs
cell cycle
43,6%
7302,0
G0-G1
68,2%
G2-M
25,6%
S
0,1%
cell cycle
99,6%
VL1-A :: VL1 PO-PRO-A
x
VL1-W :: VL1 PO-PRO-W
Compact population
97,6%
FSC-A :: FSC-A
x
SSC-A :: SSC-A
Single Cells
73,7%
FSC-A :: FSC-A
x
FSC-H :: FSC-H
Live cells
91,1%
BL3-A :: BL3 LD Red-A
x
SSC-A :: SSC-A
0 3.0M 6.0M 9.0M
cell cycle PO-PRO
10
0
10
1
10
2
10
3
10
4
10
5
10
6
EdUclickiTAF488
PO-PRO test DU145 Martina T ctrl izo.fcs
cell cycle
65,3%
8116,0
G0-G1
17,7%
G2-M
37,4%
S
38,1%
cell cycle
99,3%
VL1-A :: VL1 PO-PRO-A
x
VL1-W :: VL1 PO-PRO-W
Compact population
94,5%
FSC-A :: FSC-A
x
SSC-A :: SSC-A
Single Cells
61,4%
FSC-A :: FSC-A
x
FSC-H :: FSC-H
Live cells
85,8%
BL3-A :: BL3 LD Red-A
x
SSC-A :: SSC-A
0 3.0M 6.0M 9.0M
cell cycle PO-PRO
10
0
10
1
10
2
10
3
10
4
10
5
10
6
EdUclickiTAF488
PO-PRO test DU145 Martina T ctrl spec.fcs
cell cycle
49,4%
4935,0
G0-G1
68,6%
G2-M
27,2%
S
1,0%
cell cycle
99,1%
VL1-A :: VL1 PO-PRO-A
x
VL1-W :: VL1 PO-PRO-W
Compact population
96,1%
FSC-A :: FSC-A
x
SSC-A :: SSC-A
Single Cells
82,6%
FSC-A :: FSC-A
x
FSC-H :: FSC-H
Live cells
78,2%
BL3-A :: BL3 LD Red-A
x
SSC-A :: SSC-A
0 3.0M 6.0M 9.0M
cell cycle PO-PRO
10
0
10
1
10
2
10
3
10
4
10
5
10
6
EdUclickiTAF488
PO-PRO test DU145 Martina T mln izo.fcs
cell cycle
61,5%
3239,0
G0-G1
34,5%
G2-M
6,8%
S
52,2%
cell cycle
99,3%
VL1-A :: VL1 PO-PRO-A
x
VL1-W :: VL1 PO-PRO-W
Compact population
93,2%
FSC-A :: FSC-A
x
SSC-A :: SSC-A
Single Cells
64,7%
FSC-A :: FSC-A
x
FSC-H :: FSC-H
Live cells
84,7%
BL3-A :: BL3 LD Red-A
x
SSC-A :: SSC-A
0 3.0M 6.0M 9.0M
cell cycle PO-PRO
10
0
10
1
10
2
10
3
10
4
10
5
10
6
EdUclickiTAF488
PO-PRO test DU145 Martina T mln spec.fcs
cell cycle
50,7%
15218,0
G0-G1
72,6%
G2-M
22,9%
S
1,3%
cell cycle
99,7%
VL1-A :: VL1 PO-PRO-A
x
VL1-W :: VL1 PO-PRO-W
Compact population
98,5%
FSC-A :: FSC-A
x
SSC-A :: SSC-A
Single Cells
65,6%
FSC-A :: FSC-A
x
FSC-H :: FSC-H
Live cells
90,3%
BL3-A :: BL3 LD Red-A
x
SSC-A :: SSC-A
0 3.0M 6.0M 9.0M
cell cycle PO-PRO
10
0
10
1
10
2
10
3
10
4
10
5
10
6
EdUclickiTAF488
PO-PRO test DU145 Simona ctrl izo.fcs
cell cycle
58,1%
13479,0
G0-G1
43,5%
G2-M
1,7%
S
39,7%
cell cycle
99,5%
VL1-A :: VL1 PO-PRO-A
x
VL1-W :: VL1 PO-PRO-W
Compact population
95,7%
FSC-A :: FSC-A
x
SSC-A :: SSC-A
Single Cells
62,7%
FSC-A :: FSC-A
x
FSC-H :: FSC-H
Live cells
91,3%
BL3-A :: BL3 LD Red-A
x
SSC-A :: SSC-A
0 3.0M 6.0M 9.0M
cell cycle PO-PRO
10
0
10
1
10
2
10
3
10
4
10
5
10
6
EdUclickiTAF488
PO-PRO test DU145 Simona ctrl spec.fcs
cell cycle
54,6%
16367,0
G0-G1
68,3%
G2-M
27,5%
S
0,5%
cell cycle
99,5%
VL1-A :: VL1 PO-PRO-A
x
VL1-W :: VL1 PO-PRO-W
Compact population
98,0%
FSC-A :: FSC-A
x
SSC-A :: SSC-A
Single Cells
68,9%
FSC-A :: FSC-A
x
FSC-H :: FSC-H
Live cells
91,0%
BL3-A :: BL3 LD Red-A
x
SSC-A :: SSC-A
0 3.0M 6.0M 9.0M
cell cycle PO-PRO
10
0
10
1
10
2
10
3
10
4
10
5
10
6
EdUclickiTAF488
PO-PRO test DU145 Simona mln izo.fcs
cell cycle
61,2%
11179,0
G0-G1
33,5%
G2-M
5,2%
S
48,7%
cell cycle
99,6%
VL1-A :: VL1 PO-PRO-A
x
VL1-W :: VL1 PO-PRO-W
Compact population
97,8%
FSC-A :: FSC-A
x
SSC-A :: SSC-A
Single Cells
67,1%
FSC-A :: FSC-A
x
FSC-H :: FSC-H
Live cells
92,9%
BL3-A :: BL3 LD Red-A
x
SSC-A :: SSC-A
0 3.0M 6.0M 9.0M
cell cycle PO-PRO
10
0
10
1
10
2
10
3
10
4
10
5
10
6
EdUclickiTAF488
PO-PRO test DU145 Simona mln spec.fcs
cell cycle
60,7%
18218,0
G0-G1
43,8%
G2-M
52,1%
S
0,3%
cell cycle
98,3%
VL1-A :: VL1 PO-PRO-A
x
VL1-W :: VL1 PO-PRO-W
Compact population
92,9%
FSC-A :: FSC-A
x
SSC-A :: SSC-A
Single Cells
75,2%
FSC-A :: FSC-A
x
FSC-H :: FSC-H
Live cells
81,7%
BL3-A :: BL3 LD Red-A
x
SSC-A :: SSC-A
0 3.0M 6.0M 9.0M
cell cycle PO-PRO
10
0
10
1
10
2
10
3
10
4
10
5
10
6
EdUclickiTAF488
PO-PRO test DU145 helena ctrl iso.fcs
cell cycle
56,2%
3195,0
G0-G1
35,4%
G2-M
10,2%
S
39,3%
cell cycle
98,6%
VL1-A :: VL1 PO-PRO-A
x
VL1-W :: VL1 PO-PRO-W
Compact population
87,6%
FSC-A :: FSC-A
x
SSC-A :: SSC-A
Single Cells
63,8%
FSC-A :: FSC-A
x
FSC-H :: FSC-H
Live cells
80,7%
BL3-A :: BL3 LD Red-A
x
SSC-A :: SSC-A
0 3.0M 6.0M 9.0M
cell cycle PO-PRO
10
0
10
1
10
2
10
3
10
4
10
5
10
6
EdUclickiTAF488
PO-PRO test DU145 helena ctrl spec.fcs
cell cycle
44,4%
8823,0
G0-G1
61,7%
G2-M
33,7%
S
0,2%
cell cycle
99,0%
VL1-A :: VL1 PO-PRO-A
x
VL1-W :: VL1 PO-PRO-W
Compact population
96,7%
FSC-A :: FSC-A
x
SSC-A :: SSC-A
Single Cells
73,6%
FSC-A :: FSC-A
x
FSC-H :: FSC-H
Live cells
83,7%
BL3-A :: BL3 LD Red-A
x
SSC-A :: SSC-A
0 3.0M 6.0M 9.0M
cell cycle PO-PRO
10
0
10
1
10
2
10
3
10
4
10
5
10
6
EdUclickiTAF488
PO-PRO test DU145 helena mln iso.fcs
cell cycle
59,0%
6706,0
G0-G1
25,8%
G2-M
11,4%
S
53,2%
cell cycle
98,4%
VL1-A :: VL1 PO-PRO-A
x
VL1-W :: VL1 PO-PRO-W
Compact population
94,6%
FSC-A :: FSC-A
x
SSC-A :: SSC-A
Single Cells
57,0%
FSC-A :: FSC-A
x
FSC-H :: FSC-H
Live cells
77,5%
BL3-A :: BL3 LD Red-A
x
SSC-A :: SSC-A
0 3.0M 6.0M 9.0M
cell cycle PO-PRO
10
0
10
1
10
2
10
3
10
4
10
5
10
6
EdUclickiTAF488
PO-PRO test DU145 helena mln spec.fcs
cell cycle
41,2%
9091,0
G0-G1
43,6%
G2-M
51,2%
S
0,2%
cell cycle
99,7%
VL1-A :: VL1 PO-PRO-A
x
VL1-W :: VL1 PO-PRO-W
Compact population
96,5%
FSC-A :: FSC-A
x
SSC-A :: SSC-A
Single Cells
75,2%
FSC-A :: FSC-A
x
FSC-H :: FSC-H
Live cells
88,7%
BL3-A :: BL3 LD Red-A
x
SSC-A :: SSC-A
0 3.0M 6.0M 9.0M
cell cycle PO-PRO
10
0
10
1
10
2
10
3
10
4
10
5
10
6
EdUclickiTAF488
PO-PRO test DU145 miriam ctrl iso.fcs
cell cycle
64,2%
13426,0
G0-G1
34,0%
G2-M
13,9%
S
39,6%
cell cycle
99,4%
VL1-A :: VL1 PO-PRO-A
x
VL1-W :: VL1 PO-PRO-W
Compact population
95,7%
FSC-A :: FSC-A
x
SSC-A :: SSC-A
Single Cells
59,5%
FSC-A :: FSC-A
x
FSC-H :: FSC-H
Live cells
94,8%
BL3-A :: BL3 LD Red-A
x
SSC-A :: SSC-A
0 3.0M 6.0M 9.0M
cell cycle PO-PRO
10
0
10
1
10
2
10
3
10
4
10
5
10
6
EdUclickiTAF488
PO-PRO test DU145 miriam ctrl spec.fcs
cell cycle
53,7%
16104,0
G0-G1
60,3%
G2-M
35,7%
S
0,5%
cell cycle
99,5%
VL1-A :: VL1 PO-PRO-A
x
VL1-W :: VL1 PO-PRO-W
Compact population
97,6%
FSC-A :: FSC-A
x
SSC-A :: SSC-A
Single Cells
75,0%
FSC-A :: FSC-A
x
FSC-H :: FSC-H
Live cells
90,9%
BL3-A :: BL3 LD Red-A
x
SSC-A :: SSC-A
0 3.0M 6.0M 9.0M
cell cycle PO-PRO
10
0
10
1
10
2
10
3
10
4
10
5
10
6
EdUclickiTAF488
PO-PRO test DU145 miriam mnl iso.fcs
cell cycle
66,2%
15613,0
G0-G1
27,0%
G2-M
9,9%
S
48,0%
cell cycle
99,6%
VL1-A :: VL1 PO-PRO-A
x
VL1-W :: VL1 PO-PRO-W
Compact population
98,0%
FSC-A :: FSC-A
x
SSC-A :: SSC-A
Single Cells
67,9%
FSC-A :: FSC-A
x
FSC-H :: FSC-H
Live cells
93,7%
BL3-A :: BL3 LD Red-A
x
SSC-A :: SSC-A
0 3.0M 6.0M 9.0M
cell cycle PO-PRO
10
0
10
1
10
2
10
3
10
4
10
5
10
6
EdUclickiTAF488
PO-PRO test DU145 miriam mnl spec.fcs
cell cycle
62,1%
18643,0