•          1. Which amino acids are the most frequent at the contact surfaces of protein
interacting partners?

•          a.            polar

•          b.           charged

•          c.            hydrophobic

•          d.           aromatic

•

•          2. Which secondary structures are involved in coiled-coil binding mode?

•          a.            beta-sheets

•          b.           beta-sheets and helices

•          c.            loops

•          d.           intertwining helices

•

•          3. interactom is

•          a.            Network of protein-protein interactions (in a given organism)

•          b.           Network of interactions of a given protein

•          c.            Interaction database of all biomolecules

•          d.           Interactions involved in protein complexes

•          4. What parameters must contact surfaces of binding partners fulfill?

•          a.            they must (only) have oposite charges

•          b.           they must (only) have complementary surfaces

•          c.            they must have complementary characteristics (both shape and polarity)

•          d.           they must be hydrophobic

•          5. Provide at least 2 examples of coiled-coil containing proteins:








•          6. What is the complexom?

•          a.            network of interacting proteins in one cell

•          b.           all interactions of one protein

•          c.            all protein complexes of a given organism

•          d.           network of strong interactions

•          7. How can a post-translational modification of the protein directly influence
protein-protein interaction?

•          a.            no way to do it directly

•          b.           only via protein conformational change

•          c.            can block or enhance the interaction

•          d.           by degradation of the protein

•          8. What are the advantages of the protein complex composed of several small subunits
(compared to macromolecule composed one big protein)?

•          a.            higher dynamics, modularity, regulation

•          b.           higher protein stability

•          c.            better access to the protein

•          d.           better degradation

•          9. How does the mitochondrial ATP pump work during ADP/ATP conversion?

•          a.            transports Na^+  across the membrane

•          b.           utilizes cGMP

•          c.            transports K^+  across the membrane

•          d.           generates rotation when transporting H^+ across the membrane

•          10. Provide at least 2 examples of molecular machines:









Send your answers to: jpalecek@sci.muni.cz