Hodnocení výsledků KO
Bouřková L, OKH FN Brno
Obecná pravidla pro měření
pravidelná kontrola KO i dif
kontrola Správnosti (firemní materiál)
kontrola přesnosti (vitální krev)
porOVnatelnOSt (metodik, přístrojů, laboratoří.....)
správná údržba přístroje (kontroly po údržbě)
vždy statistické vyhodnocení: CV%, SD, Mean
• kontrola správného odběru EDTA (evt. Mg2*
nebo citrát)
Pozor na: stáří vzorku, vzhled vzorku - chilózní, ikterický...
Obecná pravidla pro hodnocení
• specifická pravidla pro daný analyzátor:
- princip měření
- rozsah hodnot měřených parametrů (linearita)
- hlášení přístroje
- měřený / počítaný parametr, grafy
• klinická hlediska:
- diagnóza
- léčba
- historie pacienta (LIS, četnost měření)
Fyziologické hodnoty Infekce
Sequence #: 703  Autoloader r38t06
Patient/Human
Specimen ID: 020003255
Name:
RETC In
X-B	WBC	RBC	PLT
In	In	In	In
WBC	7.88 Mi.	WVF	.992
S EG	4.73	*S	60.1
BAND	0.00	%BD	0.00
IG	0.00	%IO	0.00
BLST	0.00	%BL	0.00
MONe	.483	%Me	6.12
EOS	.186	%E	2.37
BASO	.051	%B	.641
lym*	2.43	%Le	30.8
VAPL 0.00		%VL	0.00
RBC	5.22 1NR/L	RBCo	5.12
HGB	9.38 mol .		
HCT	49.0 V		
MCV	93.9 ft		
MCH	1.80 fal		
MCHC	19.2 milí		
RDW	12.4 V»		
RETC	71.7 IM..	%R	1.38
IRF	.221		
NRBC 0.00 IM/l		NR/W 0.00	
PLTo	229. IM/L	PLTi	239.
MPV	9.59 ft	CD61	----
PDW	16.7 IKBOi	PLTs	----
PCT	2.19 til	PLTI	----
Test Selection; CBC+RETC Param Set(Chart Page): 1 Limit Set: 1
Run Date/Time: 04.10.99 13:34
WBC siffmntul
i ■ i Iii 7* - COlttlřXITV
L9»-Ki Ft
0"-.
i—i—i—r FL3 - DIM
Bif«4incí PIC
Bono Poly I
0'
-i—t—r-
snz
Optic*! IB
J*TC
Volt»« ((L)
ru - ww
Sequence ff: 7967  Autoloader r04t02 Patient/Human Specimen ID: 031273 Name:
Test Selection: CBC Param Set(Chart Page) Limit Set: 1
Run Date/Time: 30.04.99 08:58
X-B WBC	RBC	PLT	RETC
In       1 Out	In	In	In
WBC   31.0 IM..	WVF	.990	
17.8	y	57.5	
9.14	MD	29.5	BAND
2.06	%IG	6.65	IG
BLST 0.00	%BL	0.00	
MONe -637	%Me	2.06	
EOS .043	%E	.139	
BASO 0.00	%B	0.00	
LYMe 1.29	%Le	4.16	
VARL 0.00	%VL	0.00	
nc DifUrtntul
RBC HGB HCT MCV MCH MCHC PDW
RETC----
IRF ----
NRBC 0.00
3.39 1W2,. RBCo 3.45 6.49 aol/.
33.2 » 98.0 1.91 19.5
15.3
n '»I
MÍ'.
KV !M.
%R
IM'J. NR/W 0.00
PLIC 305. IM/.
9.00 ft
PDW  14.7 »({SS)
2.74 U
305.
Volmt (íl)
Bono Poly I
i  i i—i i  i i—r—r
o* - sro
opuui ptr
VolOM <fl)
Satelitóza trombocytů
Sequence ft:  9427   Open Tube Specimen Type/SubType:  Pat i ent/Human Specimen ID: gruyters Name:
•InvalidDat
WBC	3.20*1M/l	WVF	. 954*
	0.00*		0.00*
LYM	2.00*	%L	62.6*
MONO	. 663*	%M	20.7*
EOS	.512*	%E	16.0*
BASO	.023*	%B	. 732*
RBC	5.00 1M12/1	RBCo	4.84
HGB	9.50 Ml/L		
HCT	.455 l/l		
MCV	91 .0 fl		
MCH	1 .90 fMl		
MCHC	20.9 Ml/l		
RDW	12.4 K1		
RETC	----IM/l	%R	----
IRF	----		
NRBC	0.00* IM/l	NR/W	0.00*
PLT	156. IM/L	PLT i	162.
MPV	8.48 fl		
PDW	16.9 11(01)		
PCT	1 .32 il/l		
VARLYM .50
Test Selection: CBC Param Set(Chart Page): 1 Limit Set: 1
Run Date/Time:  26/01/99 17:44
nc Differential Dono Polp i
voltm* (fL) VelMM (fL)
Monocytární leukémie
* I n val idOata *»c mťf«t«nti»i
Mono folp
WBC	15.0 IM/l	WVF	.977
n EU	3.25s	%n	21.78
LYM	3.19»	%L	21.3*
MONO	8.36«	%M	55.9*
EOS	.024	%E	.159
BASO	.136	%B	.906 BAND
			IG
			BLAS1
RBC	3.63 Ikll/L	RBCo	3.70
HGB	6.91 Ml/l		
HCT	.308 l/l		
MCV	84.9 11		
MCH	1.90 fNl		
MCHC	22.4 ii: .		
RDW	14.5 m		
R ETC	----1M/L	%R	----
IRF	----		
NRBC	0.00 IM/l	NR/W 0.00	
PLT	25.9 IM/l	PLIi	36.1
MPV	8.93 fl		
PDW	18.9 ll(ÍM)		
.52 .59 .99
iříz :-:
Lffr-Mi n
tu - tm
WBC   207. IM/l   WVF   ^843 nvWBC
Chronická lymfatická leukémie
NEU 8.76 LIM 195.S MONO 1.62 BOS 0.00 BASO 1.34
%N %L *M %E %B
4.24 94.3s .782 0.00 .647
VARLYM
RBC 3.80 IfcllA
HGB 7.31 Ml/l
HCT .376 IA
M:v 98.9 II
MCH 1.92 tni
MCHC 19.5 Ml/l
RDW 12.9 St
R ETC----IM/l
IRF .----
NRBC 0.00 IM/l
PLTo 47.8 IM/l
::r   9.81 11
17.0 1KBI)
FBI': 3.79
%R ----
NR/W 0.00 PLTi 42.9
Mononukleóza
sic Silíiria'.iil
■ono rol; I
WBC	16.9 IM/l	WVF	.983
	4.21		24.9
	0.00	tm	0.00
	0.00		0.00
BLST	1.11	%BL	6.52 BLAST
MONe	.703	*Me	4.15
SOS	.097	\l	.570
BASO	.056	VB	.333
LYMe	10.5	*Le	62.0 FP?
VARL	.258	VVL	1.52 VARLYM
RBC	5.36	RBCo 5.11	
HGB	9.43 n: .		
HCT	.460 l/l		
MCV	85.9 fl		
MCH	1.76 M		
v -	20.4 Mi/l		
RDW	15.5 St		
R ETC	----IM/l	W	----
TCF	----		
NRBC	O.OOslM/l	NR/W O.OOs	
	248. IM/l		241.
	8.34 11		
	18.0 tlít?)		
VBC Jiíftriatul			
7?
90
—i—1—I  I   1   I I—r—ŕ     T—1—i—r—r-
v - comsn o* - she
urn n
ojtial ro
Uridine* PIT
NRBC ve vzorku
Sequence #: 7677   Open Tube
Pat i ent/Human
Specimen ID: 990812880018
Name:
X-B Jn
WBC
In
RBC Jn
PLT Jn
RETC Jn
Test Selection: CBC+R Param Set(Chart Page): 1 Limit Set: 1
Run Date/Time: 22/08/99 20:54 rac Diit«r»ntm
Chladové protilátky
WBC   7.73 IfleJ/L   WVF .957
NEU    1.86 24.1
LYM   3.74 VL 48.4
MONO 1.78 %M 23.1
BOS    .326 %E 4.22
BASO .020 %B .258
RBC HGB HCT MCV MCH
1^22810112/1 RBCo 1 .28 3.23 Ml/L
.14181/1 115.Sfl 2-65sfioi MCHC 23.08W1/1 RDW 26.5SICV
RETC.----IM/l
IRF   — -NRBC 5.07 IM/l
PLTc 290. IM/l MPV   8.31 fl
14.6 10(01) PCT   2.41 til
ASYM
-%R
NR/W 65.6
285.
WBC		10e9/L	WVF	.975*	WBC	1	1069/L	WVF	.955
SEG	1.06'		%S	43.4*	SEG	r.9T			50.7
BAND	.509*		%BD	13.3*	BAND	.463		%BD	12.1
IG	.580*		,IG	15.1*	1(3	.383		%IG	10.0
BLST	0.00*		%BL	0.00*	BLST	0.00		%BL	0.00
MONe	.434*		%Me	11.3*	MONe	.342		%Me	8.96
EOS	.073*		%E	1.90*	EOS	.064		%E	1.68
BASO	.020*		%B	.511*	BASO	.018		%B	.467
LYMe			%Le	14 4	LYMe			%Le	16.0
VARL	0.00*		%VL	0.00*	VARL	0.00		%VL	0.00
před zahřátím						po zahřátí			
RBC		10e12/L	RBCo	1.23'	RBC	2.66	10e12/L	RBCo	
HGB		g/L			HGB	92.1	g/L		
HCT		L/L			HCT		UL		
MCV	93.2*	IL	1 1		MCV	95.7	fL		
MCH	65.9*	pg	i i ■ ■		MCH	34.6	pg		
MCHC 707.*		ft			MCHC	362.	g/i		
RDW	17.9*	%cv			RDW	17.4	%CV		
RETC	196.*	10e9/L	%R	14.0*	RETC	—	10e9/L	%R	—
IRF	.471*				IRF	—			
NRBC	.109*	lOeS/L	NR/W	2.83*	NRBC	0.00	10e9/L	NR/W	0.00
PLTo	245.*	10e9/L	PLTi	260.*	PLTo	258.	10e9/L	PLTi	254.
MPV		fL	CD61	____	MPV	7 6S	fL	CD61	—
PDW	17.3*	KHGSD)	PLTs	—	Pf)W	17.0	KHGSD)	PLTs	
	1.89*		PLTI	—		1.97	mL/L	PLTI	—
VolOM ((I)
i    I    t-r-r-
volmt <fL)
Vzorek po dialýze
Sequence »: 4453  Autoloader r9H07
Patient/Human
Specimen ID: JX6223
Name:
X-B	WBC	RBC	PLT RETC	WC Diff«r«ntul	
In	In	-	In In		
			»InvalidData 4		
WBC	15.0*1W/l	WVF	.990*		
NEU	5.78*		38.5*		
LYM	9.00»	%L	59.9*		
MONO	.214«		1.43*		
EOS	.022*		.149*	1 -	
BASO	0.00*	%E	0.00*	5 I " Z I	
					
Test Selection: CBC+RETC Param Set(Chart Page): 1 Limit Set: 1
Run Date/Time: 29/06/00 12:08 Mono Pol)) I
VARLYM
RBC 3.78s10ell/l	RBCo 3.85	
HGB   8.08 NOl/l		rstRBC
HCT   .395 l/l		
MCV   104. 11		
MCH   2.13 !mI		
MCHC 20.5 HOl/l		
RDW  21.9 IC¥		
RETC 95.3S1M/1	%R 2.52s	
IRF .335		IR
NRBC O.OO*10e)/l	NR/W 0.00*	
PLTO 76.9 I0ei/l	90.3	
mpv 16.6*fL	----	
39.8*IO|SS0]	PLTs----	
1.28*il/l	PLT I----	
Itiiil lifftrntiil	!ik hfiholon	
ses [kh-	;«omi	:iicro
un :«vel0	Mira	«acso
iiip« :pro	mm	m
ioio ;n»si	:pou	[usostip
Eosii     ;mi hi	[target	
usd :rusui	Ml	:nt
pu esi	IM morph	
COMHt:		
OIFFIt:	Dite:	
IhpecUnc» (LT
•:Vftr
L»p«cUnci MC
Volum« (iL) VoluiM (tl)
HC Coin! lite Violation
Resistant HC Interference litt HC results
Unable to Find clear Separation letieei NC Subpopulatioas
lower, Upper, or Lower aid Upper Region Interference in Pili Ristojrai
Lower. Upper, or Lower and upper Region Interference in PLTi Histoc.ru
Po odstranění rstRBC
Sequence #: 4472  Autoloader r94t09
Patient/Human
Specimen ID: JX6223
Name:
Test Selection: CBC+R Param Set(Chart Page): 1 Limit Set: 1
Run Date/Time: 29/06/00 12:56
X-B In
WBC In
RBC
PLT
In
RETC In
•InvalidData
VBC Diffirtntul
Mono Poly I
WBC	6.47 IOri/l	WVF .945	
	5.55	85.7	
LľM	.531	%L 8.21	FP?
MONO	.364	%M 5.63	
EOS	.028	%E .437	
BASO	.001	%B .015	
RBC	3.74 lOeli/l	RBCo 3.78	
HGB	7.96 liol/l		
HCT	.388 L/l		
MCV	104. IL		
MCH	2.13 fHl		
MCHC	20.5 in: .		
RDW	21.9 IN		
RETC	----IM/l	vp   -—	
IRF	----		
NRBC O.OOslOeS/L		NR/W 0.00s	
PLTo	75.2 IOri/l	PLTi 81.3	
	13.2*11	----	
PDW	1 9 . 7*10(8501	PLTs----	
	.992*iL/l	PLTI----	
uiial Differential		[MC lorpholo»	
SES	!IEU	IHODJUL	HICIfl
UN	IIYELO	: POLYCH	MACRC
urn	[PRO	!im	["ISC
ION	mm	phi	[USOSTIP
EWU	:vu in	[TARGET	
USD	:ioisui		!■*
PLI ESI		[PIT MORPH	
Coeeit:			
JIFF hn Date:			
D)ip«cUnc« PLT
>p«d»nc« PJC
Volwi (iL)
voll»« (fL)
Lower, Upper, or lower and Upper Region Interference in PHi Hiitojru lower, Upper, or lower and Upper legion Interference in Pili «istogmi
Reakce na EDTA
Sequence #: 8950  Autoloader r06t03 Patient/Human Specimen ID: 030074152 Name: KATINKA JOHA PETERSEN
Test Selection: CBC Parám Set(Chart Page): Limit Set: 1
X-B In
WBC
WBC In
14.8 I' 9.64 0.00 0.00 BLST 0.00* MONe 1.27* EOS .016 BASO .010 LYMe 3.92* VARL 0.00*
RBC HGB HCT MCV MCH
3.56 Mít/l
7.09 ii:
33.0 :
92.8 fl
1.99 i:
MCHC 21.5 ml/1
RDW  11.3 n
RETC---- IW/l
IRF  -— NRBC 0. OOs10e9/L
132.*10eí7L 8.99*fL PDW 15.2*10(SSÍ) 1.18*11/1
RBC	PLT RETC	
In	In In	
	•InvalidData	
WVF	.985	
	64.9	
	0.00	
	0.00	
%BL	0.00*	0
%Me	8.54*	5
%E	.109	I
%B	.068	-t
%Le	26.4*FP?	
%VL	0.00*VARLYM	.50
RBCo	3.59	
Run Date/Time
WC Diff»r«nti»l
02.05.00 09:47
Mono Po 1)1 I
%R —-NR/W O.OOs
140.*PltClmp
lnul Differotial		HC Morphology	
SES	META	NORKU	MICRO
!»![	HELO	POLVCH	HACRO
UM	PRO	HYPOCH	AMISO
MONO	BLAST	POIK	BASOSTIP
EOSII	Hl LYN	TARGET	
USD	TOXGRAI	SPHE=C	NRBC
Pil ESI		PH MORPH	
COMent:_			
LO*-Hl PL
Optic*! PLT
Voll»« (fL)
Volu/M (fL)
Unable to Find Clear Separation ietieen ItC Stipulations Significant (inter of Cells Reioved froi MIC Differential Analysts Interference liti PLT Results Due to PLT Clmpino.
Sequence #: 2568
Specimen Type/SubType: Patient/Human
Specimen ID: IA5285
Name:
Test Selection: CBC+RETC Param Set(from Chart Page): 1 Limit Set: 1
Run Date/Time: 22/01/97 12:17
Invalid Data rac Diif«r«iti*i
Meno Pol; I
WBC 6.46*10*3.	WVF	.961*
4.14*	\]\	64.1*
LYM 1.87*	*L	29.0*
MONO .257*	>::	3.98*
EOS .189*	\l	2.93*
BASO 0.00*	•VE	0.00*BAND
RBC HGB HCT MCV MCH
5.19 1M2/. 15.7 s:. .454 .. 87.5 !. 30.2 ps MCHC 34.6 ;:. RDW  12.1 \ RETC 43.6 M. IRF .232 NRBC 0.00*1')«!J.
PLT 45.2*11*. MPV 10.5*fL PDW 16.8*l9i&! .476*-..
RBCo 5.28
%R .840 NR/W 0.00*
49.6*PltClmp
7* - COHPUinV Lw-Kl PL
Optic*! PLT
ALL a fragmenty cytoplazmy
Sequence #:   2699    Autoloader r38t02
Pat i ent/Human
Specimen ID: 000213479091
Name:
X-B In
WBC
NEU
LYM
MONO
EOS
BASO
WBC	RBC	PLT
2 Out	In	In
3.36 10e9/L	WVF	.956
. 133	%N	3 . 95
3 . 08s	%L	91 . 7s
. 136s	%M	4.05s
.010	%E	. 296
0.00	%B	0 . 00
RETC In
•InvalidData
Test Selection: CBC Param Set(Chart Page): Limit Set: 1 Run Date/Time: 21/02/00
Differential
Mono Poly I
1
08 : 57
RBC HGB HCT MCV MCH
MCHC 20.3 RDW
3.74 10C12/L
5.81 nol/L
.287 L/l
7 6.6 f I
1 . 55 fiol
HOl/L
RETC
IRF
NRBC
17.9 XCV
---- 10*9/1
0.00 10e9/L
81 . 5*10*9/1 5 .41 *fl 15. 1*10(6SO) . 441
BLAST VARLYM
RBCo 3.78
Low-Mi PL
Optic* 1 PLT
%R ----
NR/W 0.00
131.* ----
PLTs----
----
Dnp«&»nc« RBC
Impedance PLT
Volum« <ťL)
volum* (ťL>
Lower, Upper, or Lower and Upper Region Interference in PLT i Histograi PIC/POC Delti