Silkworm - Bombyx mori Silkworm (Lepidoptera, Bombycidae) Silk production Wax moth -Galleria mellonella Wax moth (Lepidoptera, Pyralidae, Gallerinae) Bee pests - feeds on wax, creates corridors in wax plates lined with fiber Excellent model organism - metamorphosis, immunity, metabolism, ... 1 2 3 4 5 6 Biuret reagent 1 ml 1 ml 1 ml 1 ml 1 ml 1 ml Standard 20 µl Blank - H2O 20 µl Hemolymph BM 20 µl Hemolymph BM 20 µl FBS 20 µl Carp / linseed serum 20 µl ... Total protein (g / l) = a. A1 … Absorbance of the sample A2 … Absorbance of the standard 70 (protein concentration in the standard)