PřF:Bi5614en Genetics in animal model organisms 2024


Lecture 1 Drosophila genetics

•      Genetics on the Fly: A Primer on the Drosophila Model System

•      KG Hales, CA Korey, AM Larracuente, DM Roberts - Genetics, 2015 - academic.oup.com

•      Read first half of this. Also history and glossary at the end

•      The joy of balancers

•      DE Miller, KR Cook, RS Hawley - PLoS genetics, 2019 - journals.plos.org

•      Short. Read all of it

•      How to Design a Genetic Mating Scheme: A Basic Training Package for Drosophila Genetics

•      St Johnston, Daniel. "The art and design of genetic screens: Drosophila
melanogaster." Nature reviews genetics 3.3 (2002): 176-188.

Lecture 2. Drosophila genetic screens for mutants affecting embryonic pattern formation

•      St Johnston, Daniel. "The art and design of genetic screens: Drosophila
melanogaster." Nature reviews genetics 3.3 (2002): 176-188.Heidelberg screen, Wieschaus and
Nusslein Vollhard

•      Wieschaus, Eric, and Christiane Nüsslein-Volhard. "The Heidelberg screen for pattern mutants
of Drosophila: a personal account." Annual Review of Cell and Developmental Biology 32.1 (2016):
1-46.

•      Scott Gilbert textbook chapter of Drosophila

Lecture 3. Drosophila maternal effect mutants; formation of eggs, AP and DV axis, homeotic mutants
and vertebrate conservations,

•      St Johnston, Daniel. "The art and design of genetic screens: Drosophila
melanogaster." Nature reviews genetics 3.3 (2002): 176-188.

•      Wieschaus, Eric, and Christiane Nüsslein-Volhard. "The Heidelberg screen for pattern mutants
of Drosophila: a personal account." Annual Review of Cell and Developmental Biology 32.1 (2016):
1-46.

•      Kimble, Judith, and Christiane Nüsslein-Volhard. "The great small organisms of developmental
genetics: Caenorhabditis elegans and Drosophila melanogaster." Developmental biology 485 (2022):
93-122.

Lecture 4. Drosophila transgenesis, GAL4/UAS and somatic recombination to generate clones of mutant
cells, germline clones and germline development

•      The art and design of genetic screens: Drosophila melanogaster

•      D St Johnston - Nature reviews genetics, 2002 - nature.com

•      [PDF] Creating mosaics in Drosophila

•      N Perrimon - International Journal of Developmental …, 1998 - genepath.med.harvard.edu

•      [PDF] GAL4 system in drosophila: A fly geneticist's swiss army knife

•      JB Duffy - genesis, 2002 - fenix.ciencias.ulisboa.pt


Lecture 5 Drosophila nervous system development, embryonic and larval neurogenesis

•      The art and design of genetic screens: Drosophila melanogaster

•      D St Johnston - Nature reviews genetics, 2002 - nature.com

•      Drosophila Embryonic CNS Development: Neurogenesis, Gliogenesis, Cell Fate, and
Differentiation

•      ST Crews - Genetics, 2019 - academic.oup.com

Lecture 6. Drosophila temporal specification in neurogenesis, larval CNS development, ecdysone

Doe, Chris Q. "Temporal patterning in the Drosophila CNS." Annual review of cell and developmental
biology 33.1 (2017): 219-240.

Truman, James W., and Lynn M. Riddiford. "Drosophila postembryonic nervous system development: a
model for the endocrine control of development." Genetics 223.3 (2023): iyac184.

Lecture 8 Drosophila vision and movement

(Lecture 7 Powerpoint file is missing and was never prepared. The file uploaded as Lect 7 is
actually Lect 6 with audio)

Currier, Timothy A., Michelle M. Pang, and Thomas R. Clandinin. "Visual processing in the fly, from
photoreceptors to behavior." Genetics 224.2 (2023): iyad064.

Lecture 9 Drosophila Olfaction, learning and memory

Montell, Craig. "Drosophila sensory receptors—a set of molecular Swiss Army Knives." Genetics 217.1
(2021): 1-34.

Davis, Ronald L. "Learning and memory using Drosophila melanogaster: a focus on advances made in
the fifth decade of research." Genetics 224.4 (2023): iyad085.

Lecture 10 Drosophila Learning and memory, Internal states, Circadian rythms and sleep

Modi, Mehrab N., Yichun Shuai, and Glenn C. Turner. "The Drosophila mushroom body: from
architecture to algorithm in a learning circuit." Annual review of neuroscience 43.1 (2020):
465-484.

Dubowy, Christine, and Amita Sehgal. "Circadian rhythms and sleep in Drosophila
melanogaster." Genetics 205.4 (2017): 1373-1397.

Lecture 11 C. elegans genetic screens, microRNAs, RNAi and

Corsi, Ann K., Bruce Wightman, and Martin Chalfie. "A transparent window into biology: a primer on
Caenorhabditis elegans." Genetics 200.2 (2015): 387-407.

Kimble, Judith, and Christiane Nüsslein-Volhard. "The great small organisms of developmental
genetics: Caenorhabditis elegans and Drosophila melanogaster." Developmental biology 485 (2022):
93-122.

Jorgensen, Erik M., and Susan E. Mango. "The art and design of genetic screens: Caenorhabditis
elegans." Nature Reviews Genetics 3.5 (2002): 356-369.

Lecture 12 Vertebrate genetic screens. Zebrafish and mice.

Holtzman, Nathalia G., et al. "Learning to fish with genetics: a primer on the vertebrate model
Danio rerio." Genetics 203.3 (2016): 1069-1089.

Patton, E. Elizabeth, and Leonard I. Zon. "The art and design of genetic screens:
zebrafish." Nature Reviews Genetics 2.12 (2001): 956-966.