ABCHc Biochemistry - practice

Faculty of Medicine
autumn 2021
Extent and Intensity
0/1/0. 1 credit(s). Type of Completion: z (credit).
doc. RNDr. Jiří Dostál, CSc. (seminar tutor)
doc. Mgr. Zdenka Fohlerová, Ph.D. (seminar tutor)
Mgr. David Galuška (seminar tutor)
Mgr. Jana Gregorová, Ph.D. (seminar tutor)
doc. PharmDr. Jiří Kos, Ph.D. (seminar tutor)
MUDr. Michaela Králíková, Ph.D. (seminar tutor)
RNDr. Hana Paulová, CSc. (seminar tutor)
doc. Mgr. Ondřej Peš, Ph.D. (seminar tutor)
Mgr. Jiří Slanina, Ph.D. (seminar tutor)
Mgr. Jindra Smutná, Ph.D. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
Mgr. Marie Tomandlová, Ph.D. (seminar tutor)
Mgr. Jan Víteček, Ph.D. (seminar tutor)
Martina Hanousková (assistant)
Bc. Ivana Ševčíková (assistant)
Guaranteed by
MUDr. Michaela Králíková, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Timetable of Seminar Groups
ABCHc/BPC1_BIO_01: Thu 16. 9. 8:00–9:40 A16/212, Thu 30. 9. 8:00–9:40 A16/212, Thu 14. 10. 8:00–9:40 A16/212, Thu 11. 11. 8:00–9:40 A16/212, Thu 25. 11. 8:00–9:40 A16/212, Thu 9. 12. 8:00–9:40 A16/212, Z. Fohlerová
ABCHc/BPC1_BIO_02: Thu 16. 9. 8:00–9:40 A16/211, Thu 30. 9. 8:00–9:40 A16/211, Thu 14. 10. 8:00–9:40 A16/211, Thu 11. 11. 8:00–9:40 A16/211, Thu 25. 11. 8:00–9:40 A16/211, Thu 9. 12. 8:00–9:40 A16/211, J. Víteček
ABCHc/BPC1_BIO_03: Thu 23. 9. 10:00–11:40 A16/216, Thu 7. 10. 10:00–11:40 A16/216, Thu 21. 10. 10:00–11:40 A16/216, Thu 4. 11. 10:00–11:40 A16/216, Thu 18. 11. 10:00–11:40 A16/216, Thu 2. 12. 10:00–11:40 A16/216, Thu 16. 12. 10:00–11:40 A16/216, J. Kos
ABCHc/BPC1_BIO_04: Thu 23. 9. 10:00–11:40 A16/212, Thu 7. 10. 10:00–11:40 A16/212, Thu 21. 10. 10:00–11:40 A16/212, Thu 4. 11. 10:00–11:40 A16/212, Thu 18. 11. 10:00–11:40 A16/212, Thu 2. 12. 10:00–11:40 A16/212, Thu 16. 12. 10:00–11:40 A16/212, Z. Fohlerová
ABCHc/BPC1_BIO_05: Thu 16. 9. 10:00–11:40 A16/212, Thu 30. 9. 10:00–11:40 A16/212, Thu 14. 10. 10:00–11:40 A16/212, Thu 11. 11. 10:00–11:40 A16/212, Thu 25. 11. 10:00–11:40 A16/212, Thu 9. 12. 10:00–11:40 A16/212, Z. Fohlerová
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Subject is complementary to the Biochemistry lecture ABCHp. Students become familiar with the background and equipments of chemical laboratory. They will learn to perform simple laboratory operations. During experiments students verify some findings discussed in theoretical lessons.
Learning outcomes
After completing the course, the student will:
- be familiar with safety and health protection at work in the laboratory;
- be able to calculate the concentration of solutions, pipette, prepare or dilute a solution of a given concentration;
- be acquainted with the work with biological material and its processing in the laboratory of clinical biochemistry;
- be able to work with basic instruments in the biochemical laboratory, eg. pH meters, spectrophotometers;
- understand the principle and implementation of selected methods used in clinical biochemistry (optical, chromatographic, electrophoretic, immunochemical methods).
  • Introductory information, safety rules. Preparing solutions of the given concentration. Biological material. Examinations in clinical biochemistry. Biochemical analyzers. The measurement of pH of various solutions. pH-metres. Spectrophotometry, absorption curve. Determination of glucose. Chromatography. Gel chromatography. Adsorption chromatography of plant pigments (silicagel). HPLC demonstration. Electrophoretic and immunochemical methods. Proteins in blood serum and urine. Electrophoresis of blood serum proteins.
    required literature
  • Podklady pro cvičení v IS.
  • Podklady pro práci ve cvičení jsou uloženy v interaktivní osnově na IS MUNI.
    recommended literature
  • Michaela KRÁLÍKOVÁ, Hana PAULOVÁ a kolektiv autorů. Biochemie pro biomedicínské techniky. 1. vyd. Brno: Masarykova univerzita, 2021. 177 s. ISBN 978-80-210-9857-2
Teaching methods
practical exercises, presentation of laboratory methods and instruments.
Assessment methods
Full attendance in all lessons (making up all missing and justified lessons), completion of all lab reports is the condition for the course-unit credit. Obtaining of course-unit credits of practicals is the pre-requisite for registration to the examination.
Language of instruction
Further comments (probably available only in Czech)
The course is taught annually.
Information on the extent and intensity of the course: 15.
Teacher's information
Laboratory coat, respirator,laboratory gloves and overshoes are necessary. Attendance in practicals is obligatory. All absences must be justified through the Department of study affairs. Illness or some other serious reason are usually the only acceptable excuse for absence from lesson and must be officially confirmed. After being absent the student must make up for the lesson. Absences that are not apologized are not allowed to make up. Student is obliged to prepare himself for each lesson. Students that are not sufficiently prepared will not be admitted in participating in the lesson.
The course is also listed under the following terms Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2022, autumn 2023, autumn 2024.
  • Enrolment Statistics (autumn 2021, recent)
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