VLLM0522c Medical Microbiology II - practice

Faculty of Medicine
autumn 2024
Extent and Intensity
0/3/0. 3 credit(s). Type of Completion: z (credit).
MUDr. Ondřej Zahradníček (lecturer)
MUDr. Lenka Černohorská, Ph.D. (seminar tutor)
Mgr. Milada Dvořáčková, Ph.D., MBA (seminar tutor)
Mgr. Monika Dvořáková Heroldová, Ph.D. (seminar tutor)
MUDr. Jan Flodr (seminar tutor)
Ing. Veronika Holá, Ph.D. (seminar tutor)
Mgr. Ing. Veronika Korecká (seminar tutor)
Mgr. Dominika Polaštík Kleknerová (seminar tutor)
Mgr. Katarína Rebrošová (seminar tutor)
prof. MUDr. Filip Růžička, Ph.D. (seminar tutor)
MUDr. Alena Siváková (seminar tutor)
RNDr. Kateřina Snopková, Ph.D. (seminar tutor)
Mgr. Lukáš Vacek, Ph.D. (seminar tutor)
doc. MUDr. Vladana Woznicová, Ph.D. (seminar tutor)
Iva Holešovská (assistant)
Guaranteed by
prof. MUDr. Filip Růžička, Ph.D.
Department of Microbiology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: MUDr. Ondřej Zahradníček
Supplier department: Department of Microbiology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
VLLM0421c Med.Microbiol. && ( VLBC0422c Biochemistry II - practice || VSBC041c Biochemistry II -pract. ) && ( VLBC0422s Biochemistry II - seminar || VSBC041s Biochemistry II -s )
Necessary for subject absolution is knowledge of topics from VLLM0421c. This knowledge is considered obvious and topics of first semester will not be explained repeatedly.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the subject is to acquaint students with the basics of diagnostic and therapeutic approach in the most typical microbial diseases. This basic knowledge will then be broadened in clinical subjects (especially Infectology). Those interested in deeper knowledge in antibiotic problematic and clinical microbiology may later register to the subject VSAT081 (Basics of antimicrobial therapy)
Learning outcomes
At the end of the course student
* defines microbial findings in different localisations
* defines the difference between common microbial flora, temporary findings, contaminants and pathogens in various localisations
* records infections that are rare and the rather common ones
* defines basics of diagnostic and therapeutic approach in the most typical microbial disease; here, basis is only supposed, more enlargement is supposed in other subjects, namely Infectology

Namely student defines normal flora (if it does exist)/transitory findings/contaminants/pathogens in
* individual parts of upper and lower respiratory ways, lungs and middle ear
* different parts of gastroitestinal tract, livers, bile bladder and pancreas
* individual parts of uropoetic system
* individual parts of reproductive system
* individual parts of central and peripherial neural system
* bloodstream, bones, muscles and different tissuess
* skin, eyes, extarnal ear

Student also describes specific problems of microbial infections
* during pregnancy and delivery and during first days after delivery
* in hospitalized, especially immunocompromised persons (healthcare associated infections)
Student also composes the basic facts in in
* problematics of biofilm detection (including testing of effectivity of antibiotics to biofilm)
* basic processi of clinical microbiology (indication, sampling, material transport, specimen processing, result releasing, result interpretation)
  • Week 1: Introduction to clinical microbiology
  • Week 2 Staphylococci; Bloodstream infections
  • Week 3 Streptococci, enterococci; Upper respiratory tract infections
  • Week 4: G+ rods; Superficial wound infections
  • Week 5: Enterobacterales I; Urinary tract infections
  • Week 6: Enterobacterales II, campylobacters, helicobacters, GIT infections
  • Week 7: G- non-fermenters, burns and healthcare associated infections
  • Week 8: Haemophili, neisseriae, bacterial meningitis
  • Week 9: Anaerobic bacteria, deep wound and organ infections
  • Week 10: Mycobacteria, actinomycetes, nocardiae; culturable LRW infections
  • Week 11: Borreliosis, aseptic meningitis and encephalitis
  • Week 12: Syphilis and more STIs
  • Week 13: Various Gram-negative bacteria, zoonoses, infections of mother and child
  • Week 14: Revision
  • VOTAVA, Miroslav. Lékařská mikrobiologie obecná. (General Medical Microbiology.). 1st ed. Brno: Neptun, 2001, 247 pp. ISBN 80-902896-2-2. info
  • VOTAVA, Miroslav. Lékařská mikrobiologie speciální (Medical microbiology special). 1st ed. Brno: NEPTUN, Březová 18, 637 00 Brno, 2003, 495 pp. ISBN 80-902896-6-5. info
  • VOTAVA, Miroslav, Filip RŮŽIČKA, Vladana WOZNICOVÁ, Lenka ČERNOHORSKÁ, Milada DVOŘÁČKOVÁ, Monika DVOŘÁKOVÁ HEROLDOVÁ, Veronika HOLÁ and Ondřej ZAHRADNÍČEK. Lékařská mikrobiologie: Vyšetřovací metody (Medical Microbiology: Laboratory Procedures). 1st ed. Brno: Neptun, 2010, 495 pp. ISBN 978-80-86850-04-7. info
  • GREENWOOD, David. Lékařská mikrobiologie : přehled infekčních onemocnění : patogeneze, imunita, laboratorní diagnostika a epidemiologie. 1. vyd. Praha: Grada Publishing, 1999, 686 s. ISBN 80-7169-365-0. info
  • RULÍK, Martin, Veronika HOLÁ, Filip RŮŽIČKA, Miroslav VOTAVA, Dana BAUDIŠOVÁ, Jiří GALLO, Simona KAPRÁLOVÁ, Kateřina KOHUŠOVÁ, Dagmar KOUKALOVÁ, Václav KŮDELA, Jiří MIKEŠ, Radko NOVOTNÝ, Martina SIGLOVÁ, Věra TORŠOVÁ and Jiří ZIMÁK. Mikrobiální biofilmy (Microbial Biofilms). 1. vydání. Olomouc: Univerzita Palackého v Olomouci, 2011, 448 pp. Monografie. ISBN 978-80-244-2747-8. info
  • Základní jsou tituly 1, 2 a veškeré materiály z IS MU, ostatní jsou doplňkové.
Teaching methods
practical training
self-study with e-learning materiasls
Assessment methods
Conditions for credits:
(1) absolving all practicals, with following notes:
- it is possible to substitute withing the week of tuition, if the capacity of the room allows it; no special formalities are required for it
- one absence is allowed according to Study Rules, but even in this case appology is recommended, and completing the konwledgen and the protocol required
- one more absence, if justified or approved by teacher, is possible under the same conditions
- more absences than two (although justified!) usually require some form of substitution (e. g. essay making or so)
usually there are no substitutions organized at the end of semester
- absences that are neiter officially justified nor consulted with the teacheer in advance are not allowed
(2) complete laboratory protocol (usually signed by the teacher; teacher would not sign a protocol that is not satisfactory; if so, student has to redo it)
(3) succesfully filled in ROPOT questions to all topics (full number of points)
(4) succesfully written final test, date of the credit test will be declared in time. Number of questions is 12, "single best answer" type, 8/12 correct answers required, 7/12 is sufficient for student having all ROPOTs filled in in time (in time = before the corresponing lesson; this is checked several times during the semester)
Language of instruction
Follow-Up Courses
Further comments (probably available only in Czech)
Information on completion of the course: Podmínky zápočtu jsou uvedeny v Doplňkových údajích k předmětu
The course is taught annually.
The course is taught: every week.
Information on the extent and intensity of the course: 45.
Listed among pre-requisites of other courses
Teacher's information
E-mail and phone contact:
Ass. prof. Filip Růžička, MD, PhD: fruzic@fnusa.cz, phone: 543 183 090 (head)
Ass. prof. Vladana Woznicová, MD, PhD: woznic@med.muni.cz
Secretary: Martina Ondříková, phone no: +420 543 183 091
Ondřej Zahradníček, MD: zahradnicek@fnusa.cz (contact person), phone no: +420 543 183 113
Veronika Holá, Ing, PhD: veronika.hola@fnusa.cz
Lenka Černohorská, MD, PhD.: lenka.cernohorska@fnusa.cz, TEL 543 183 093
Monika Dvořáková Heroldová, MSc, PhD: monika.heroldova@fnusa.cz
Milada Dvořáčková, MSc: milada.dvorackova@fnusa.cz
Lukáš Vacek, MSc: vacek.lukas.88@gmail.com
Alena Siváková, MD: sivakova.alena@marsik.org
More information in the practical Hall of the institute.
The course is also listed under the following terms Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023.
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