Bi9250cen Special Methods of Immunology – Practical Course

Faculty of Science
autumn 2021
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Milan Číž, Ph.D. (seminar tutor)
doc. Mgr. Lukáš Kubala, Ph.D. (seminar tutor)
Guaranteed by
doc. Mgr. Lukáš Kubala, Ph.D.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: doc. RNDr. Martin Vácha, Ph.D.
Supplier department: Department of Experimental Biology - Biology Section - Faculty of Science
Prerequisites
(! Bi9250c Special Immunol. Meth. - pr. ) && ! NOWANY ( Bi9250c Special Immunol. Meth. - pr. ) && NOW ( Bi5220en Immunology )
Subject is connected to the basic practice course in immunology that is why students are supposed to have finished the basic practice course in immunology. The course is to extended the theory and praxis of basic immunology subject in its methods.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should be able to: understand and explain basic methods in immunology; use data about these methods in praxis; make reasoned decisions about the method ELISA; interpret its practical knowledge in labs; understand and explain the basic immunological techniques; work with information on important current immunological methods used in immunological labs and in research;
Learning outcomes
At the end of the course students should be able to: understand and explain basic methods in immunology; use data about these methods in praxis; make reasoned decisions about the method ELISA; interpret its practical knowledge in labs; understand and explain the basic immunological techniques; work with information on important current immunological methods used in immunological labs and in research;
Syllabus
  • The practice proceeds in the form of individual parts of methods that are solved: 1. putting of antigen on the surface of microtitration plates and their preparation for ELISA test • 2. testing on the presence of antibodies against chosen pathogen in serum • 3. Test for the detection of lymphocyte activity • 4. Imunochromatographic tests - test for the detection of the presence of HIV, RSV antibodies in sera • 5. Test of indirect agglutination. Presence of erytrhrocytes Ag in saliva • 6. Immunocytology - invertebrate immunology, immune cells of insects
Literature
    recommended literature
  • HARLOW, Ed and David LANE. Antibodies : a laboratory manual. New York: Cold Spring Harbor Laboratory, 1988. xiii, 726. ISBN 0-87969-314-2. info
Teaching methods
laboratory exercising; using some of simple and difficult immunological methods.
Assessment methods
The students participation at lectures is mandatory. The final classification is in the form of written test at the end of term. The students are recommended to fill in written self-tests offered during the term.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

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