Bi9393cen Analytical Cytometry – Practical Course

Faculty of Science
autumn 2021

The course is not taught in autumn 2021

Extent and Intensity
0/1/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Karel Souček, Ph.D. (seminar tutor)
Guaranteed by
Mgr. Karel Souček, Ph.D.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology - Biology Section - Faculty of Science
Prerequisites (in Czech)
(! Bi9393c Anal.cytometry - Pract.course ) && ! NOWANY ( Bi9393c Anal.cytometry - Pract.course ) && NOW ( Bi9393en Analytical Cytometry )
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in basic methods of flow cytometry and image analysis.
Learning outcomes
The student will learn basic methods of cell analysis using flow cytometry including sample preparation acquisition and data analysis.
Syllabus
  • Basic training in operation of routine flow cytometer FACSCalibur. Setup, calibration, quality control. Basic training in operation of high speed sorter. Setup, calibration, quality control. Cell cycle analysis. Immunophenotype analysis. GFP expression analysis and sorting. Data analysis - software FlowJo.
Literature
    recommended literature
  • SHAPIRO, Howard M. Practical flow cytometry. 4th ed. Hoboken: John Wiley & Sons, 2003. xlix, 681. ISBN 0471411256. info
Teaching methods
practical training
Assessment methods
Evaluation of protocols.
Language of instruction
English
Further Comments
The course is taught annually.
The course is taught: in blocks.

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