Bi9393en Analytical Cytometry

Faculty of Science
autumn 2021

The course is not taught in autumn 2021

Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
Mgr. Karel Souček, Ph.D. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Guaranteed by
Mgr. Karel Souček, Ph.D.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology - Biology Section - Faculty of Science
Prerequisites
(! Bi9393 Analytical cytometry ) && ! NOWANY ( Bi9393 Analytical cytometry )
No limitation for enrollment.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, fluorescent imaging techniques; - understand basic principles of data analysis, their interpretation and presentation.
Learning outcomes
The student will learn detail principles of flow cytometry and image analysis including practical applications of these methodologies.
Syllabus
  • *Flow cytometry general introduction - basic principles and history *Principles of flow cytometry and sorting -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation * Biological application of flow cytometry -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology, Flow cytometric methods in cell death analysis * Application of flow cytometry in clinical immunology and hematology. Basic principles of the image analysis.
Literature
    recommended literature
  • SHAPIRO, Howard M. Practical flow cytometry. 4th ed. Hoboken: John Wiley & Sons, 2003. xlix, 681. ISBN 0471411256. info
Teaching methods
The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
Assessment methods
Course consists of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
Language of instruction
English
Further Comments
The course is taught annually.
The course is taught: every week.

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