C7920 Structure and functions of proteins

Faculty of Science
Autumn 2018
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Alternate Types of Completion: k (colloquium).
Teacher(s)
prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
Mgr. Tomáš Klumpler, Ph.D. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)
Supervisor
prof. RNDr. Břetislav Brzobohatý, CSc.
National Centre for Biomolecular Research - Faculty of Science
Supplier department: National Centre for Biomolecular Research - Faculty of Science
Prerequisites
Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
Fields of study the course is directly associated with
there are 9 fields of study the course is directly associated with, display
Course objectives
The course makes students familiar with structural motifs recurring in protein structures and their relation to biological functions of proteins, and their engineering.
Learning outcomes
At the end of the course students should know principles of protein structure and function. In the first part, individual structural motifs recurring in protein structures are described, and ways are shown how proteins with quite unrelated functions are built up from combinations of these. In the second part, particular biological functions are considered and the different protein structural solutions are discussed that have evolved to fulfill them. In the third part, the basic techniques of protein structural biology are introduced and examples of engineering protein structure and function are shown.
Syllabus
  • 1. Basic structural principles of protein architecture. The building blocks of proteins. Motifs of protein structure. Domain structure of proteins. 2. The roles of individual motifs of protein structure in protein biological functions. DNA interacting proteins, transcription factors, receptors. Molecular recognition of foreign molecules by immune system. Membrane proteins, membrane receptors. Enzyme catalysis. Prediction, engineering, and design of protein structures. Determination of protein structure. 3. Genetic engineering techniques for structure-function analysis in proteins. Recombinant DNA construction. Gene isolation and cloning. Gene expression regulating elements. DNA sequencing. In vitro mutagenesis. Recombinant protein production in heterologous expression systems.
Literature
  • BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998. xiv, 410 s. ISBN 0-8153-2304-2. info
Teaching methods
lectures
Assessment methods
Oral exam or colloquium.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
Teacher's information
http://ncbr.chemi.muni.cz/~jiri/courses.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2018/C7920

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