Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
Fields of study the course is directly associated with
there are 9 fields of study the course is directly associated with, display
The course makes students familiar with structural motifs recurring in protein structures and their relation to biological functions of proteins, and their engineering.
At the end of the course students should know principles of protein structure and function. In the first part, individual structural motifs recurring in protein structures are described, and ways are shown how proteins with quite unrelated functions are built up from combinations of these. In the second part, particular biological functions are considered and the different protein structural solutions are discussed that have evolved to fulfill them. In the third part, the basic techniques of protein structural biology are introduced and examples of engineering protein structure and function are shown.
1. Basic structural principles of protein architecture. The building blocks of proteins. Motifs of protein structure. Domain structure of proteins. 2. The roles of individual motifs of protein structure in protein biological functions. DNA interacting proteins, transcription factors, receptors. Molecular recognition of foreign molecules by immune system. Membrane proteins, membrane receptors. Enzyme catalysis. Prediction, engineering, and design of protein structures. Determination of protein structure. 3. Genetic engineering techniques for structure-function analysis in proteins. Recombinant DNA construction. Gene isolation and cloning. Gene expression regulating elements. DNA sequencing. In vitro mutagenesis. Recombinant protein production in heterologous expression systems.
BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998. xiv, 410 s. ISBN 0-8153-2304-2. info