PřF:S2003 Methods plant cytogenetics 2 - Course Information
S2003 Methods in plant cytogenetics and cytogenomics II. - practical courseFaculty of Science
- Extent and Intensity
- 0/24. 2 credit(s) (plus extra credits for completion). Type of Completion: z (credit).
- RNDr. Terezie Mandáková, Ph.D. (lecturer)
- Guaranteed by
- prof. Mgr. Martin Lysák, Ph.D., DSc.
National Centre for Biomolecular Research - Faculty of Science
Contact Person: RNDr. Terezie Mandáková, Ph.D.
Supplier department: National Centre for Biomolecular Research - Faculty of Science
- S2002 Methods plant cytogenetics 1
The completion of the basic course "Methods in plant cytogenetics and cytogenomics I. (S2002)" is a prerequisite for enrolling this advanced course.
- Course Enrolment Limitations
- The course is offered to students of any study field.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
- Course objectives
- Students will get acquainted with preparation of extended DNA fibres (fibre FISH), immuno localization of modified histones, immuno-labeling of 5-methyl cytosine, and FISH to immuno preparations. Advanced methods of fluorescence microscopy and digital photomicrography will be practised.
- Learning outcomes
- Student will be able to (i) prepare extended DNA fibres, (ii) prepare DNA probes, (iii) carry out fibre FISH, immuno-localization of modified histones, immuno-labeling of 5-methyl cytosine and FISH to immuno preparations, (iv) analyze preparations using fluorescence microscopy, (v) photograph chromosomes and cell nuclei, and finally (vi) analyze and process the acquired photographs.
- Annotation This advanced course follows Methods in plant cytogenetics I. Aim of the course is to make students familiar with the principles and applications of DNA-DNA fluorescence in situ hybridization (FISH) and immuno-localisation as tools for studying the chromatin and nuclear organization of higher plants. Students will get acquainted with preparation of extended DNA fibres (fibre FISH), immuno-localization of modified histones, immuno-labeling of 5-methyl cytosine, and FISH to immuno-preparations. Advanced methods of fluorescence microscopy and digital photomicrography will be practised. Syllabus Day 1 - introduction in theoretical and practical principles of nuclei isolation and DNA fibre FISH - nuclei isolation from leaves - nuclei extension and fibre FISH hybridization with probes corresponding to ribosomal DNA repeats Day 2 - introduction in multilayer detection of the fluorescence signals and immuno-localisation of modified histones and methylated DNA - fibre FISH multilayer detection - immuno-localisation of modified histones H3metK4 and H3metK9: PART I - immuno-labelling of 5-methyl cytosine on mitotic and meiotic chromosomes - fluorescence microscopy of fibre FISH and immuno-labelled methylcytosine Day 3 - immuno-localisation of modified histones H3metK4 and H3metK9: PART II - fluorescence microscopy of modified histones - advanced analysis using fluorescence microscopy, image capture - computer processing of microscope images (Adobe Photoshop) - conclusions
- recommended literature
- SCHWARZACHER, T. and HESLOP-HARRISON. Practical in situ hybridization. Bios, 2000. info
- Teaching methods
- Lectures followed by practical tasks in a cytogenetic laboratory.
- Assessment methods
- Colloquium. Successful completion is conditioned by a timely delivery of all required protocols and completion of the final interview.
- Language of instruction
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: in blocks.
- Enrolment Statistics (recent)
- Permalink: https://is.muni.cz/course/sci/spring2021/S2003