VLIM051c Immunology - practice

Faculty of Medicine
autumn 2019
Extent and Intensity
0/2/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
MUDr. Jan Baroš (seminar tutor)
doc. MUDr. Tomáš Freiberger, Ph.D. (seminar tutor)
MUDr. Roman Hakl, Ph.D. (seminar tutor)
MUDr. Eva Hlaváčková (seminar tutor)
MUDr. Zita Chovancová, Ph.D. (seminar tutor)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
prof. MUDr. Jindřich Lokaj, CSc. (seminar tutor)
Guaranteed by
prof. MUDr. Jiří Litzman, CSc.
Department of Clinical Immunology and Allergology - Institutions shared with St. Anne's Faculty Hospital - Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Supplier department: Department of Clinical Immunology and Allergology - Institutions shared with St. Anne's Faculty Hospital - Faculty of Medicine
Timetable of Seminar Groups
VLIM051c/01: Thu 8:00–9:40 N01021
VLIM051c/02: Thu 8:00–9:40 N01021
VLIM051c/03: Wed 8:00–9:40 N01021
VLIM051c/04: Wed 8:00–9:40 N01021
VLIM051c/05: Mon 10:00–11:40 N01021
VLIM051c/06: Mon 10:00–11:40 N01021
VLIM051c/07: Fri 10:00–11:40 N01021
VLIM051c/08: Fri 10:00–11:40 N01021
VLIM051c/09: Tue 10:00–11:40 N01021
VLIM051c/10: Tue 10:00–11:40 N01021
VLIM051c/11: Fri 8:00–9:40 N01021
VLIM051c/12: Fri 8:00–9:40 N01021
VLIM051c/13: Tue 8:00–9:40 N01021
VLIM051c/14: Tue 8:00–9:40 N01021
VLIM051c/15: Wed 10:00–11:40 N01021
VLIM051c/16: Wed 10:00–11:40 N01021
Prerequisites (in Czech)
( VLBC0422p Biochemistry I -lect. || VSBC0221p Biochemistry I -lect. ) && ( VSFY0422p Physiology II - lecture || VLFY0422p Physiology II - lecture )
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
Fields of study the course is directly associated with
Course objectives
After finishing this course the student will understand the pathogenesis of immunopathological diseases and obtain the basic knowledge that he/she will aply in diagnosis and treatment of the most common disturbances of the immune system. In the laboratory part the students will be introduced to the techniques used in immunological laboratory, both serological (agglutination, precipitation, immunofluorescence, ELISA, RIA) and cellular (flow cytometry, lymphocyte proliferation tests, neutophil function assessment) to allow him to interpret the most common laboratory immunology methods in medicine. In the clinical part the mechanisms leading to immunopathological diseases such as clinical manifestation of those diseases will be discussed to allow the students to apply their knowledge in clinical medicine. The student will also discuss immunological problems associated with transplantation and malignancies. Selected articles dealing with immunological topics will be analysed and critically interpreted by the students.
Learning outcomes
The student will get acquainted with the basic methods of serological immunological diagnostics;
The student will be acquainted with the basic methodologies for examining the number and function of the immune system cells;
The student will understand the importance, principle and clinical value of the diagnostic tests necessary for the diagnosis of allergic, autoimmune and immunodeficiency diseases;
The students will become acquainted with immunological laboratory aspects of transplantation and tumor growth.
Syllabus
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins. Laboratory markers of inflammation. Blood collection. Diagnostic antisera.
  • Immunoglobulins and their biological functions. Monoclonal antibodies as diagnostic and therapeutic tools. Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum. Direct and indirect agglutination. Precipitation. Double immune diffusion. Turbidimetry, nephelometry. Immunofluorescence tests. Enzyme analysis (ELISA). RIA. Blotting techniques. Circulating and localised immune complexes. Complement system evaluation
  • Major histocompatibility complex: structure, function. HLA typing. Molecular genetic in immunology.
  • Innate or natural immunity: phagocytosis, NK cells, cytotoxicity, interferon system, complement system, acute phase reaction, inflammation. Complement system evaluation.
  • Cells of the immune system CD nomenclature of leukocyte antigens. Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity. Evaluation of phagocytic cells.
  • Primary and secondary immunodeficiency disorders. HIV disease.
  • Active and passive immunisation. Immunoglobulin therapy. Immunostimulation.
  • Autoimmunity and disease. Immunological tolerance. Autoantibodies and their laboratory detection. Clinically significant organ specific and organ non-specific autoantibodies.
  • Hypersensitivity reactions - anaphylactic, cytotoxic, immune complex, T-cell-mediated.
  • Clinical allergology. Allergic skin and exposition tests. Skin tests for early hypersensitivity. Delayed-type hypersensitivity tests. Anergy. Prevention and treatment of allergic diseases. Anaphylactic shock.
  • Transfusion immunology. ABO system. Rh system. Transfusion reactions. Immunology of organ and bone marrow transplantation. Graft vs. host reaction
  • Tumor immunology. Tumor antigens. Immune response to tumors. Immunodiagnosis of tumors. Immune-related malignancies. Paraproteins, detection, clinical significancy. Electrophoresis, Immunoelectrophoresis, immunofixation. Immunophenotyping of leukemia. Clinical significance of paraproteinemia
Literature
    recommended literature
  • LITZMAN, Jiří, Zita CHOVANCOVÁ, Vojtěch THON, Marcela VLKOVÁ, Vlastimil KRÁL and Tomáš FREIBERGER. Základy vyšetření v klinické imunologii (Investigations in clinical immunology). 1. vyd. Brno: Masarykova univerzita, 2015. 71 pp. ISBN 978-80-210-7853-6. info
  • HOŘEJŠÍ, Václav, Jiřina BARTŮŇKOVÁ, Tomáš BRDIČKA and Radek ŠPÍŠEK. Základy imunologie. 6., aktualizované vydání. V Praze: Stanislav Juhaňák - Triton, 2017. 297 stran. ISBN 9788075532503. info
  • CHAPEL, Helen, Mansel HAENEY, Siraj A. MISBAH and Neil SNOWDEN. Základy klinické imunologie : 6. vydání. Translated by Vojtěch Thon. Kroměříž: Triton, 2018. xiv, 343. ISBN 9788075533968. info
Teaching methods
The course is given in a form of seminars and demonstration of laboratory methods. A home reading of selected articles to prepare their presentations, discussion and critical assessment is also included.
Assessment methods
Credit
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on the extent and intensity of the course: 30.
The course is also listed under the following terms Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018.
  • Enrolment Statistics (recent)
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