aVLBI0222c Biology II - practice

Faculty of Medicine
spring 2020
Extent and Intensity
0/3/0. 3 credit(s). Type of Completion: z (credit).
Ing. Lívia Eiselleová, Ph.D. (lecturer)
prof. RNDr. Ondřej Slabý, Ph.D. (lecturer)
Mgr. Kateřina Cetkovská, Ph.D. (seminar tutor)
Mgr. Kateřina Francová, Ph.D. (seminar tutor)
Sebastiano Giallongo (seminar tutor)
Master Alexandru Nită (seminar tutor)
Mgr. Markéta Nováková, Ph.D. (seminar tutor)
Sara Poovakulathu Abraham, MSc (seminar tutor)
Mgr. Petra Pospíšilová, Ph.D. (seminar tutor)
Nicola Silva, PhD. (seminar tutor)
prof. MUDr. Iva Slaninová, Ph.D. (seminar tutor)
Jana Růžičková (assistant)
Guaranteed by
prof. MUDr. David Šmajs, Ph.D.
Department of Biology - Theoretical Departments - Faculty of Medicine
Contact Person: Ing. Lívia Eiselleová, Ph.D.
Supplier department: Department of Biology - Theoretical Departments - Faculty of Medicine
Timetable of Seminar Groups
aVLBI0222c/30: Mon 17. 2. to Fri 15. 5. Fri 10:30–11:00 B07/214, Fri 11:00–12:30 B07/214, Fri 11:00–12:30 B07/217, Fri 12:30–13:00 B07/214
aVLBI0222c/31: Mon 17. 2. to Wed 13. 5. Wed 8:00–8:30 B07/214, Wed 8:30–10:00 B07/214, Wed 8:30–10:00 B07/217, Wed 10:00–10:30 B07/214
aVLBI0222c/32: Mon 17. 2. to Tue 12. 5. Tue 13:00–13:30 B07/214, Tue 13:30–15:00 B07/214, Tue 13:30–15:00 B07/217, Tue 15:00–15:30 B07/214
aVLBI0222c/33: Mon 17. 2. to Thu 14. 5. Thu 9:30–10:00 B07/214, Thu 10:00–11:30 B07/214, Thu 10:00–11:30 B07/217, Thu 11:30–12:00 B07/214
aVLBI0222c/34: Mon 17. 2. to Mon 11. 5. Mon 8:00–8:30 B07/214, Mon 8:30–10:00 B07/214, Mon 8:30–10:00 B07/217, Mon 10:00–10:30 B07/214
aVLBI0222c/35: Mon 17. 2. to Tue 12. 5. Tue 10:00–10:30 B07/214, Tue 10:30–12:00 B07/214, Tue 10:30–12:00 B07/217, Tue 12:00–12:30 B07/214
aVLBI0222c/36: Mon 17. 2. to Fri 15. 5. Fri 12:00–12:30 B07/205a, Fri 12:30–14:00 B07/217, Fri 12:30–14:00 B07/205a, Fri 14:00–14:30 B07/205a
aVLBI0222c/37: Mon 17. 2. to Thu 14. 5. Thu 12:30–13:00 B07/214, Thu 13:00–14:30 B07/214, Thu 13:00–14:30 B07/217, Thu 14:30–15:00 B07/214
aVLBI0222c/38: Mon 17. 2. to Wed 13. 5. Wed 13:00–13:30 B07/214, Wed 13:30–15:00 B07/217, Wed 13:30–15:00 B07/214, Wed 15:00–15:30 B07/214
aVLBI0222c/39: Mon 17. 2. to Mon 11. 5. Mon 14:00–14:30 B07/214, Mon 14:30–16:00 B07/217, Mon 14:30–16:00 B07/214, Mon 16:00–16:30 B07/214
Prerequisites (in Czech)
aVLBI0121c Biology I - pract. && aVLBI0121s Biology I - seminar
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The main objectives of the course are:
- learning the methods of risk evaluation for hereditary diseases of various molecular background in patients based on family anamneses
- understanding the basic principles of gene engineering
- understanding and practicing the selected molecular methods used for examination of some cancers or hereditary and infectious diseases with regard to selection of appropriate therapy
Learning outcomes
After completing the course, the student will be able to:
- evaluate the risk of hereditary diseases of various molecular background in patients based on assigned family anamneses of the diseases
- describes the basic principles of genetic prognosis and genetic counselling
- suggest and perform basic molecular-genetic diagnostic examinations of some hereditary, tumour and infectious diseases (PCR, restriction digest, gel electrophoresis), and to interpret the obtained results
- explain selected basic techniques of gene engineering and their application in medicine, and, with knowledge of the used method, to select the clones of cells with an inserted gene
- explain the basic laboratory tests used in genetic toxicology and the overall use of genetic toxicology, and to interpret the results obtained by selected methods
  • Week 1: Genetic disorders - gonosomal inheritance
  • Week 2: Extensions to basic genetics
  • Week 3: Genetic prognosis and genetic counselling
  • Week 4: Human population genetics
  • Week 5: Control test 3 (knowledge from week 13 of the autumn semester to week 4 of the spring semester - lectures, seminar, practices)
  • Week 6: Molecular diagnostics of cancer with respect to treatment options
  • Week 7: Gene engineering – production of human proteins in bacterial cells
  • Week 8: Molecular analysis of predispositions to cardiovascular disease in humans
  • Week 9: Molecular diagnostics of human pathogenic bacteria
  • Week 10: Restriction mapping of DNA
  • Week 11: Genetic toxicology
  • Week 12: substitution practices – substitutions for public holidays
  • Week 13: Control test 4 (knowledge from week 5 to week 12 of the spring semester - lectures, practices)
  • Week 14: dissection week – no practices
  • Week 15: dissection week – no practices
    required literature
  • Protocols for practices are provided in electronic form in the Information System of the Masaryk University in the Study Materials of the course aVLBI0222c
Teaching methods
laboratory practice, class discussion
Assessment methods
Practical classes are mandatory.
Requirements for the course completion: full attendance in the practices (1 absence, excused or unexcused, is allowed); all protocols completed (also for missed lessons) and checked and signed by appropriate teachers; successfully passed 2 written control tests during the semester (20 questions each, 4 possibilities, 1 answer correct, student need to reach at least 12 points out of 20 in each test). In case you do not pass one of the control tests and its re-sit, you have to pass the final test (Credit test) based on knowledge of the entire semester lectures and practices. The test comprises of 20 questions: 10 test questions (multiple answers can be correct, negative marking is obtained for incorrect answers) + 10 given terms for a written explanation. At least 10 points out of 20 are needed to pass.
In case of student’s late arrival to the practice or poor activity at the practice, the student is required to write an essay in the length of 2 pages on a given topic. Same for the case of 2nd and any additional absence in practices (excused or unexcused). Find the instructions for essay writing in the Course-related instructions in the IS. Bring the essay printed to the teacher who specified the topic, and be ready to answer the teacher's questions regarding the topic.
In case of 3 absences, (or more than 3 absences all excused by the Office for Studies within 5 days from the beginning of the absence and introduced to the Information System), it is addressed individually and course-unit credit is awarded only after the proof of the student's knowledge of the entire semester topics (Credit test, see above).
In the case of 4 or more unexcused absences, the course-unit credit is not awarded.
Language of instruction
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
Information on the extent and intensity of the course: 45.
Teacher's information
What to bring to each practice: printed protocol for the respective practice, your own writing materials. Download the protocol for each practice from the Study materials in the IS, and study it in advance. Lab shoes, lab coats and disposable gloves are not required, but for the tasks with staining lab coats are recommended.

Substitution of practices: In exceptional cases (illness, appointment at the doctor’s or foreign policy, 1 or 2 times per semester as maximum) student can arrange a substitution with another group. To arrange a substitution, write an e-mail to the contact person of the course at least 24 hours before your planned substitution and specify the group (date and time) you would like to attend instead. Substitution is only possible on the same teaching week. When the capacity of the group you intended to substitute with is fully used, the substitution does not need to be approved. Repeated substitution with other groups is not allowed.

Complete Biology instructions to be found at the following link: https://is.muni.cz/auth/el/1411/jaro2020/aVLBI0222c/op/Biology_practices_instructions.pdf

The course is also listed under the following terms Spring 2016, Spring 2017, Spring 2018, spring 2019, spring 2021.
  • Enrolment Statistics (spring 2020, recent)
  • Permalink: https://is.muni.cz/course/med/spring2020/aVLBI0222c