aVLIM051c Imunologie - cvičení

Lékařská fakulta
podzim 2019
Rozsah
0/2/0. 30. 3 kr. Ukončení: z.
Vyučující
doc. MUDr. Tomáš Freiberger, Ph.D. (cvičící)
MUDr. Roman Hakl, Ph.D. (cvičící)
MUDr. Eva Hlaváčková (cvičící)
MUDr. Zita Chovancová, Ph.D. (cvičící)
prof. MUDr. Jiří Litzman, CSc. (cvičící)
Garance
prof. MUDr. Jiří Litzman, CSc.
Ústav klinické imunologie a alergologie - Společná pracoviště s Fakultní nemocnicí u sv. Anny v Brně - Lékařská fakulta
Kontaktní osoba: prof. MUDr. Jiří Litzman, CSc.
Dodavatelské pracoviště: Ústav klinické imunologie a alergologie - Společná pracoviště s Fakultní nemocnicí u sv. Anny v Brně - Lékařská fakulta
Rozvrh seminárních/paralelních skupin
aVLIM051c/30: Pá 1. 11. 15:00–16:40 N02011, Pá 15. 11. 15:00–16:40 N02011
aVLIM051c/31: Pá 1. 11. 15:00–16:40 N02011, Pá 15. 11. 15:00–16:40 N02011
aVLIM051c/32: Pá 1. 11. 15:00–16:40 N02011, Pá 15. 11. 15:00–16:40 N02011
aVLIM051c/33: Pá 1. 11. 15:00–16:40 N02011, Pá 15. 11. 15:00–16:40 N02011
aVLIM051c/34: Pá 8. 11. 15:00–16:40 N02011, Pá 22. 11. 15:00–16:40 N02011
aVLIM051c/35: Pá 8. 11. 15:00–16:40 N02011, Pá 22. 11. 15:00–16:40 N02011
aVLIM051c/36: Pá 8. 11. 15:00–16:40 N02011, Pá 22. 11. 15:00–16:40 N02011
aVLIM051c/37: Pá 8. 11. 15:00–16:40 N02011, Pá 22. 11. 15:00–16:40 N02011
Předpoklady
( aVLBC0422p Biochemie II - předn. || VSBC041p Biochemie II - předn. ) && ( VSFY0422p Fyziologie II - přednáška || aVLFY0422p Fyziologie II - přednáška )
Omezení zápisu do předmětu
Předmět je určen pouze studentům mateřských oborů.
Mateřské obory
Cíle předmětu
After finishing this course the student will understand the pathogenesis of immunopathological diseases and obtain the basic knowledge that he/she will aply in diagnosis and treatment of the most common disturbances of the immune system. In the laboratory part the students will be introduced to the techniques used in immunological laboratory, both serological (agglutination, precipitation, immunofluorescence, ELISA, RIA) and cellular (flow cytometry, lymphocyte proliferation tests, neutophil function assessment) to allow him to interpret the most common laboratory immunology methods in medicine. In the clinical part the mechanisms leading to immunopathological diseases such as clinical manifestation of those diseases will be discussed to allow the students to apply their knowledge in clinical medicine. The student will also discuss immunological problems associated with transplantation and malignancies.
Výstupy z učení
The student will get acquainted with the basic methods of serological immunological diagnostics;
Th student will be acquainted with the basic methodologies for examining the number and function of the immune system cells;
The student will understand the importance, principle and clinical value of the diagnostic tests necessary for the diagnosis of allergic, autoimmune and immunodeficiency diseases;
The students will become acquainted with immunological laboratory aspects of transplantation and tumor growth.
Osnova
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins. Laboratory markers of inflammation. Blood collection. Diagnostic antisera.
  • Immunoglobulins and their biological functions. Monoclonal antibodies as diagnostic and therapeutic tools. Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum. Direct and indirect agglutination. Precipitation. Double immune diffusion. Turbidimetry, nephelometry. Immunofluorescence tests. Enzyme analysis (ELISA). RIA. Blotting techniques. Circulating and localised immune complexes. Complement system evaluation
  • Major histocompatibility complex: structure, function. HLA typing. Molecular genetic in immunology.
  • Innate or natural immunity: phagocytosis, NK cells, cytotoxicity, interferon system, complement system, acute phase reaction, inflammation. Complement system evaluation.
  • Cells of the immune system CD nomenclature of leukocyte antigens. Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity. Evaluation of phagocytic cells.
  • Autoimmunity and disease. Immunological tolerance. Autoantibodies and their laboratory detection. Clinically significant organ specific and organ non-specific autoantibodies.
  • Immunoelectrophoresis, immunofixation. Immunophenotyping of leukemia. Clinical significance of paraproteinemia
Literatura
    doporučená literatura
  • ABBAS, Abul K., Andrew H. LICHTMAN a Shiv PILLAI. Cellular and molecular immunology. 7th ed. Philadelphia: Elsevier/Saunders, 2012. x, 545. ISBN 9780808924258. info
Výukové metody
Výuka je prováděna zejména formou seminářů a demontrací laboratorních metod.
Metody hodnocení
interview
Vyučovací jazyk
Angličtina
Informace učitele
Kontaktní osoba: Prof. MUDr. J. Litzman, CSc., tel: +420 54318 3125 Presence in 90% of seminars with active participation in discussions is required. The knowledge is continuously checked by oral questions.
Další komentáře
Studijní materiály
Předmět je vyučován každoročně.
Předmět je zařazen také v obdobích podzim 2017, podzim 2018.
  • Statistika zápisu (nejnovější)
  • Permalink: https://is.muni.cz/predmet/med/podzim2019/aVLIM051c