F1MB1_16 Microbiology

Faculty of Pharmacy
Autumn 2020
Extent and Intensity
2/2/0. 5 credit(s). Type of Completion: zk (examination).
Taught partially online.
Teacher(s)
doc. MUDr. Josef Feit, CSc. (lecturer)
PharmDr. Dominik Rotrekl, Ph.D. (lecturer)
PharmDr. Jakub Treml, Ph.D. (lecturer)
doc. MUDr. Josef Feit, CSc. (seminar tutor)
Ing. Marcela Nejezchlebová (seminar tutor)
Mgr. Bc. Daniela Nykodýmová (seminar tutor)
PharmDr. Dominik Rotrekl, Ph.D. (seminar tutor)
PharmDr. Alice Sychrová, Ph.D. (seminar tutor)
PharmDr. Gabriela Škovranová, Ph.D. (seminar tutor)
PharmDr. Jakub Treml, Ph.D. (seminar tutor)
Guaranteed by
PharmDr. Jakub Treml, Ph.D.
Department of Molecular Pharmacy – Departments – Faculty of Pharmacy
Supplier department: Department of Molecular Pharmacy – Departments – Faculty of Pharmacy
Timetable
Mon 13:15–14:45 Posluchárna, 135
  • Timetable of Seminar Groups:
F1MB1_16/01: Tue 11:30–13:00 Laboratoř praktických cvičení, 323, Tue 11:30–13:00 Učebna botaniky, 224, Tue 11:30–13:00 Laboratoř praktických cvičení, 324, M. Nejezchlebová, D. Rotrekl
F1MB1_16/02: Tue 13:15–14:45 Laboratoř praktických cvičení, 323, Tue 13:15–14:45 Učebna botaniky, 321, Tue 13:15–14:45 Laboratoř praktických cvičení, 324, D. Nykodýmová, D. Rotrekl
F1MB1_16/03: Tue 15:00–16:30 Učebna botaniky, 321, Tue 15:00–16:30 Laboratoř praktických cvičení, 323, Tue 15:00–16:30 Laboratoř praktických cvičení, 324, M. Nejezchlebová, D. Nykodýmová
F1MB1_16/04: Tue 16:45–18:15 Učebna botaniky, 321, Tue 16:45–18:15 Laboratoř praktických cvičení, 324, Tue 16:45–18:15 Laboratoř praktických cvičení, 323, M. Nejezchlebová
F1MB1_16/05: Wed 8:45–10:15 Laboratoř praktických cvičení, 324, Wed 8:45–10:15 Laboratoř praktických cvičení, 323, Wed 8:45–10:15 Učebna botaniky, 221, G. Škovranová, J. Treml
F1MB1_16/06: Wed 10:30–12:00 Laboratoř praktických cvičení, 323, Wed 10:30–12:00 Učebna botaniky, 221, Wed 10:30–12:00 Laboratoř praktických cvičení, 324, G. Škovranová, J. Treml
Prerequisites (in Czech)
FAKULTA ( FaF ) || OBOR ( MUSFaF )
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 160 student(s).
Current registration and enrolment status: enrolled: 104/160, only registered: 0/160
fields of study / plans the course is directly associated with
Course objectives
The subject provides an overview of pharmaceutical and medicinal aspects of microbiology, virology, mycology and parasitology. It brings basic information on the structure, function, genetics and taxonomy of medically important bacteria, viruses, fungi, yeast and parasites. The subject informs on Good Laboratory Practice and Good Manufacturing Practice. It focuses on basic methods of prevention, prophylaxis and treatment of infectious diseases and provides information on clinically significant infections in human medicine.
Learning outcomes
Basic knowledge of microbiology forming the basis for follow-up subjects Methods of molecular biology, Biotechnology of drugs, Exercises from drug biotechnology, Pharmacogenomics.
Syllabus
  • Syllabus of the lectures
    1. Introduction, information for the students. Structure of bacterial cells. Multiplication of bacteria, sporulation, germination.
    2. Immunology.
    3. Immunology and therapy, vaccination, biological therapy.
    4. Bacteriology I.
    5. Bacteriology II.
    6. Antibacterial agents. Microbial resistance. Basics of rational therapy of infectious diseases.
    7. Virology I.
    8. Virology II.
    9. Antiviral agents.
    10. Basics of medical mycology.
    11. Basics of medical parasitology.
    12. Antiparasitic and antifungal agents.
    13. Final test.

    Syllabus of the seminaries
    1. Safety precautions in microbiological laboratory, laboratory praxis, disinfection, sterilization.
    2. Disinfecting and antiseptic substances. Cultivation media. Common microbiota of human body.
    3. Diagnostic approaches I.: evaluation of colonies, staining and demonstration of selected bacteria, cultivation of bacteria, bacterial growth, identification and isolation of bacteria.
    4. Diagnostic approaches II.: biochemical reactions, MALDI-TOF, McFarland standards for turbidity of bacterial suspensions, CFU-colony forming units, evaluation of bacterial concentration by cultivation, sensitivity testing, MIC, MBC, EUCAST.
    5. Antisepsis in drug production, antimicrobial drug control.
    6. Resistance and synergy of bacteria. Biofilm.
    Practicals:
    1. Preparation of cultivation media, evaluation of selected disinfection and antiseptic substances on microorganism of group 1.
    2. Evaluation of effectivity of disinfecting substances, evaluation of bacterial colonies, Gram staining, isolation inoculation.
    3. Assessment of microbial resistance, microdilution method (MIC), disc diffusion test (microorganism group 1).
    4. Evaluation of the disc diffusion test and MIC, establishing of the MBC, establishing of the bacterial concentration in the specimen by cultivation (microorganisms group 1).
    5. Evaluation of the MBC, determination of bacterial concentration in unknown specimen.
    6. Final classification test.
Literature
    required literature
  • Votava, M. Lékařská mikrobiologie speciální. Brno, Neptun, 2003. info
  • Votava, M. Lékařská mikrobiologie obecná. Brno, Neptun, 2001. info
  • Čížek, A. Praktika z mikrobiologie pro farmaceuty. Brno, ÚMI FVL VFU Brno, 2001. info
    recommended literature
  • Buchta, V. Základy mikrobiologie a parazitologie pro farmaceuty. Karolinum, 1998. info
    not specified
  • Bednář, M. Lékařská mikrobiologie. Praha, Marvil, 1999. info
  • Hugo, W. B., Russel, A. D. Pharmaceutical microbiology. Blackwell Science, 1998. info
Teaching methods
Lectures and theoretical seminars that prepare for laboratory exercises. The output of the exercise are independently processed protocols.
Assessment methods
Předmět je ukončeno písemnou zkouškou.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
Teacher's information
To obtain the credit, students must fulfill the obligatory 100 % attendance of practices. To pass the exam, students must successfully write the classification test (min. 60 %) on the regular date. The correction tests shall have a form of oral exam.
The course is also listed under the following terms Autumn 2021, Autumn 2022, Autumn 2023.
  • Enrolment Statistics (Autumn 2020, recent)
  • Permalink: https://is.muni.cz/course/pharm/autumn2020/F1MB1_16