Bi2060c Basic microbiology - practice

Faculty of Science
Spring 2020
Extent and Intensity
0/1/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Barbora Břenková (seminar tutor)
Mgr. Iva Buriánková, Ph.D. (seminar tutor)
Mgr. Barbora Burýšková (seminar tutor)
Ivan Kushkevych (seminar tutor)
Mgr. Anna Molíková (seminar tutor)
Mgr. Martin Struk (seminar tutor)
Mgr. Lukáš Fidrich (assistant)
doc. Mgr. Monika Vítězová, Ph.D. (alternate examiner)
Guaranteed by
Mgr. Iva Buriánková, Ph.D.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: Ing. Jiřina Kučerová, Ph.D.
Supplier department: Department of Experimental Biology - Biology Section - Faculty of Science
Timetable of Seminar Groups
Bi2060c/01_1: Tue 16:00–17:50 E25/111, B. Břenková
Bi2060c/01_2: Tue 16:00–17:50 E25/111, I. Kushkevych, A. Molíková
Bi2060c/02_1: Wed 14:00–15:50 E25/111, M. Struk
Bi2060c/02_2: Wed 14:00–15:50 E25/111, B. Burýšková
Bi2060c/03_1: Wed 16:00–17:50 E25/111, A. Molíková
Prerequisites (in Czech)
NOW ( Bi2060 Basic microbiology ) && ! NOWANY ( Bi4091c Pract. from gener. microbiol. , Bi5710c Microbiology - pr. , B4090c General microbiology - pr. ) && ! Bi4091c Pract. from gener. microbiol. && ! Bi5710c Microbiology - pr. && ! Bi4090c General microbiology - pr.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
there are 22 fields of study the course is directly associated with, display
Course objectives
At the end of this course, students should be able to work aseptically, to inoculate and cultivate bacteria, to prepare stained and native microscopic slides, to determine number of bacteria in different samples, to isolate bacterial group from chosen samples, to work with yeasts, to use some basic identification methods.
Learning outcomes
At the end of the practical course of general microbiology students should be able to perform basic methods used in microbiological laboratory (methods of sterile inoculation and cultivation, slides preparation and microscopic examination of microorganisms, determination of the number of bacteria in different samples).
Syllabus
  • 1. Introduction, work safety, organization of exercises.
  • 2. Isolation, inoculation and cultivation of microorganisms.
  • 3. Microscopic examination of bacteria (slides preparation, Gram - staining, microscopy and image acquisition).
  • 4. Direct determination of cell number in Bürker chamber, vital test, S. cerevisiae.
  • 5. Microorganisms in soil.
  • 6. Determination of susceptibility of bacteria to antibiotics.
  • 7. Reports, test.
Literature
  • JANDOVÁ, Božena a Ludmila KOTOUČKOVÁ. Praktikum z mikrobiologie. 1. vyd. Brno: Vydavatelství MU, 1996. 67 s. ISBN 80-210-1374-5.
  • SKRIPTA KE CVIČENÍ Z OBECNÉ MIKROBIOLOGIE, CYTOLOGIE A MORFOLOGIE BAKTERIÍ Mgr. Jana Kopecká, Ph.D. Mgr. Gabriela Rotková, Ph.D., MASARYKOVA UNIVERZITA ELPORTÁL
Teaching methods
Topic of each practical is at first explained and demonstrated to students. Then students are solving the practical task by themselves. The outcome of each practical is written report.
Assessment methods
Active participation at practical classes, written reports, written test, credit
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019.
  • Enrolment Statistics (recent)
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