PřF:Bi5599 App. Biochem. and Cell Methods - Course Information
Bi5599 Applied Biochemistry and Cell Biology MethodsFaculty of Science
- Extent and Intensity
- 2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
- prof. RNDr. Jan Vondráček, Ph.D. (lecturer)
RNDr. Miroslav Machala, CSc. (lecturer)
prof. Mgr. Vítězslav Bryja, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
Mgr. Jiřina Medalová, Ph.D. (lecturer)
RNDr. Vendula Pospíchalová, PhD. (lecturer)
Mgr. Karel Souček, Ph.D. (lecturer)
- Guaranteed by
- prof. Mgr. Vítězslav Bryja, Ph.D.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: prof. RNDr. Jan Vondráček, Ph.D.
Supplier department: Department of Experimental Biology - Biology Section - Faculty of Science
- Mon 17. 9. to Fri 14. 12. Wed 9:00–10:50 B11/333
- Prerequisites (in Czech)
- Bi4020 Molecular biology && C3580 Biochemistry
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Course objectives
- The main goal of the course is to enable students to understand the importance of individual analytical and biochemical techniques and to interpret their own experimental data, based on the techniques taught in the course. They should be able to work with the information in the primary literature, in order to understand and implement the techniques described in primary scientific literature in the area of animal physiology and cell biology. Based on this, they will be able to present and explain the techniques used in their own work to expert audience.
- Learning outcomes
- At the end of the course, students will be able to: identify and describe basic methodology for determination of protein levels and localization, estimation of protein-protein interactions, describe basic principles of manipulation of gene/protein expression and function; describe principles of isolation of nucleic acids and their analyses; describe methodology for analyses of lipids, saccharides and small organic molecules based on chromatography and mass spectrometry; describe basic cell culture techniques; describe omics technologies and basic methods of analytical cytometry; describe possibilities for application of animal models in cell biology and physiology;
- 1) Tissue and cell culture, cultivation and analysis of cells; 2) Fluorescence techniques – fluorescence microscopy, flow cytometry, antibodies and their application in cell biology; 3) DNA isolation, separation techniques, PCR techniques, plasmid cloning and isolation, transfection of eukaryotic cells, manipulation of gene expression; 4) RNA isolation, detection (qRT-PCR, RNA-seq), non-coding RNAs, si/shRNA applications; 5) Protein analyses - separation techniques, Western blotting, immunochemistry, protein-protein interactions (immunoprecipitation); 6) Analyses of protein modifications, localization, proteomics (mass spectrometry), manipulation of protein level/activity; 7) Lipids and polysaccharides - isolation, separation and identification; 8) Separation and analytical techniques – HPLC, GC, mass spectrometry; 9) Omics techniques – genomics, proteomics, metabolomics, databases and large data sets; 10) Application of in vivo mammalian models in physiology and cell biology;
- Klouda, Moderní analytické metody, Pavel Klouda, Ostrava, 2003
- Alberts et al.: Molecular Biology of the Cell, 4th ed., Garland Science, 2002
- Wilson et al.: Principles and Techniques of Practical Biochemistry, 5th ed., CUP, 2000
- Teaching methods
- Assessment methods
- The course is finished by written exam. The test includes about 12 questions, the duration of the test is 60 min.
- Language of instruction
- Further comments (probably available only in Czech)
- Study Materials
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
- Listed among pre-requisites of other courses