Bi5599 Applied Biochemistry and Cell Biology Methods

Faculty of Science
Autumn 2019
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
prof. Mgr. Vítězslav Bryja, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
Mgr. Pavel Kadeřávek, Ph.D. (lecturer)
RNDr. Miroslav Machala, CSc. (lecturer)
Mgr. Jiřina Medalová, Ph.D. (lecturer)
RNDr. Vendula Pospíchalová, PhD. (lecturer)
Mgr. Karel Souček, Ph.D. (lecturer)
Guaranteed by
prof. Mgr. Vítězslav Bryja, Ph.D.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: Mgr. Jiřina Medalová, Ph.D.
Supplier department: Department of Experimental Biology - Biology Section - Faculty of Science
Wed 9:00–10:50 A11/333
Prerequisites (in Czech)
Bi4020 Molecular biology && C3580 Biochemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The main goal of the course is to enable students to understand the importance of individual chemical/biochemical techniques and to interpret both their own experimental data, based on the techniques taught in the course. They should be able to work with the information in the primary literature, in order to understand and implement the techniques described in primary scientific literature in the area of animal physiology and molecular physiology. Based on this, they will be able to present and explain the techniques used in their own work to expert audience.
Learning outcomes
At the end of the course, students will be able to: identify and describe basic methodology for determination of protein levels and localization, estimation of protein-protein interactions, functional analysis of protein domains, interactions of proteins with DNA; describe basic principles of manipulation of protein expression and function; describe principles of isolation of nucleic acids and determination of gene expression; describe methodology for analyses of lipids and small organic molecules based on chromatography and mass spectrometry; describe basic chemical and biochemical methods used in primary literature.
  • 1)Protein analysis; separation techniques; Western blotting, protein-protein interactions; protein localization; protein post-translational modifications; proteomics; manipulations of protein expression.
  • 2)Basic immunochemistry techniques.
  • 3)DNA isolation, separation; PCR techniques, plasmid work, transfection of eukaryotic cells.
  • 4)RNA isolation, detection (Northern blotting, RT-PCR), microarrays, siRNA.
  • 5)Lipids and polysaccharides; isolation, separation and identification.
  • 6)Low-molecular-weight compounds - separation and analytical techniques; HPLC techniques and their modifications.
  • 7)Specific cellular assays.
  • 8)Fluorescence techniques.
  • 9)Application of in vivo mammalian models in physiology. 10) Drug delivery systems 11) Principles of NMR
  • Alberts et al.: Molecular Biology of the Cell, 4th ed., Garland Science, 2002
  • Klouda, Moderní analytické metody, Pavel Klouda, Ostrava, 2003
  • Wilson et al.: Principles and Techniques of Practical Biochemistry, 5th ed., CUP, 2000
Teaching methods
lectures, class discussion
Assessment methods
Course is finished by written test going through the methods demonstrated. Test contains usually 20 questions and at least 60% of correct answers is necessary to pass.
Language of instruction
Further comments (probably available only in Czech)
Study Materials
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2020.
  • Enrolment Statistics (Autumn 2019, recent)
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