Bi7015 Chemical properties, structure and interactions of nucleic acids

Faculty of Science
Autumn 2018
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
doc. RNDr. Miroslav Fojta, CSc. (lecturer)
prof. RNDr. Emil Paleček, DrSc. (lecturer)
doc. Mgr. Miloslava Fojtová, CSc. (lecturer)
prof. RNDr. Michaela Vorlíčková, DrSc. (lecturer)
Ing. Zuzana Ferenčíková, Ph.D. (assistant)
Mgr. Ivana Kupčíková, DiS. (assistant)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: doc. RNDr. Miroslav Fojta, CSc.
Supplier department: Department of Experimental Biology - Biology Section - Faculty of Science
Timetable
Mon 24. 9. to Fri 14. 12. Thu 8:00–9:50 A11/305
Prerequisites
Basics of general and physical chemistry, organic chemistry and biochemistry, molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
At the end of the course, students should be able to explain chemical-, biochemical-, and physical-chemical features of nucleic acids; he/she will understand the nature of their interactions with small molecules as well as macromolecules, such as proteins and connections with their electrochemical behaviour. Based on acquired knowledge, students will be capable of making reasoned deductions on biological consequences of structural features of nuclei acids and their possible analytical utilization.
Syllabus
  • 1. Introduction. Nucleic acids - history. 2. DNA double helix. Physical properties and conformations of DNA. Isolation and characterisation of nucleic acids. 3. Super-helical DNA. Local DNA structures stabilised by superhelicity. 4. Covalent interactions of DNA with small molecules. DNA damage. 5. Reversible interactions of DNA with small molecules. 6. RNA structure. 7. Oligonucleotides and their analogues. 8. Interactions DNA-proteins. 9. Nucleases, DNA topoisomerases, DNA helicases, DNA ligases. 10. p53 protein and its interactions with DNA. 11. Structure and interactions of DNA in biomedicine. Gene therapy. Immunology of nucleic acids. 12. Methods for analyses of nucleic acids. 13. Electrochemistry on nucleic acids, DNA biosensors.
Literature
  • BLACKBURN, M. G. and M. J. GAIT. Nucleic Acids in Chemistry and Biology. Oxford: Oxford University Press, 1996. info
  • SINDEN, Richard R. DNA structure and function. San Diego: Academic Press, 1994. xxiii, 398. ISBN 0126457506. info
  • ADAMS, R. L. P., J. T. KNOWLER and D. P. LEADER. The Biochemistry of Nucleic Acids. 10th edit. London: Chapman and Hall, 1986. info
Teaching methods
Lectures
Assessment methods
Teaching method: lectures Type of exam: written test focused on general knowledge followed by oral exam
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2019.
  • Enrolment Statistics (Autumn 2018, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2018/Bi7015