PřF:Bi8080c Plant molecular physiology - Course Information

Bi8080c Plant molecular physiology - practice

Extent and Intensity

0/2/0. 2 credit(s). Type of Completion: z (credit).
Taught in person.

Teacher(s)

doc. Mgr. Markéta Šámalová, Ph.D. (seminar tutor)

Guaranteed by

doc. Mgr. Markéta Šámalová, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Timetable

Mon 19. 2. to Sun 26. 5. Mon 15:00–16:50 C13/332

Prerequisites (in Czech)

NOW ( Bi8080 Plant molecular physiology )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Experimental Plant Biology (programme PřF, N-EBR)
• Molecular Biology and Genetics (programme PřF, N-EXB)

Course objectives

The subject follows on from the course Bi6120 Plant explants, in which students were introduced to individual methods of in vitro cultivation. It expands knowledge towards the use of these methods in basic plant research and biotechnological applications. The subject will focus on the basic methods of molecular biology used for the preparation of genetically modified organisms (GMOs), including transgenic plants, and targeted modification of plant genome (e.g. CRISPR/Cas9).

Learning outcomes

After completing this course, the student will acquire theoretical and practical knowledge and be able to: • understand and describe the main methods used in molecular biology for the preparation of GMOs • master the preparation of transgenic plants and their analysis, including fluorescence and confocal microscopy • understand and analyze benefits and risks of modern plant biotechnologies.

Syllabus

• • Basic procedure for the preparation of genetically modified plants • Genomic databases, primer design, PCR • Isolation of plasmid DNA, its cleavage and electrophoresis • Basics of cloning • Techniques for the transformation of E. coli and agrobacteria • Transformation of tobacco by the in vitro method • Transformation of Arabidopsis thaliana by the floral dip method • Chemically inducible systems, GUS staining • Selection of transformants • Fluorescent proteins and fluorescence microscopy • Method of targeted genome modification (CRISPR/Cas9) • Transient expression in tobacco and confocal microscopy • Benefits and risks of GMOs, legislation.

Literature

• Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method. Zhang, X., Henriques, R., Lin, S.-S., Niu, Q.-W. and Chua, N.-H. NATURE PROTOCOLS 2006, 1:1-6.
• Editing plant genomes with CRISPR/Cas9. Belhaj, K., Chaparro-Garcia, A., Kamoun, S., Patron, N.J. and Nekrasov, V. Current Opinion in Biotechnology 2015, 32:76–84.
• ŠÁMALOVÁ, Markéta, C. KIRCHHELLE and I. MOORE. Universal Methods for Transgene Induction Using the Dexamethasone-Inducible Transcription Activation System pOp6/LhGR in Arabidopsis and Other Plant Species. 2019. ISSN 2379-8068. Available from: https://dx.doi.org/10.1002/cppb.20089. URL info
• Applied plant cell biology : cellular tools and approaches for plant biotechnology. Edited by Peter Nick - Zdeněk Opatrný. Berlin: Springer, 2014, xiv, 481. ISBN 9783642417863. info
• ALBERTS, Bruce, Alexander JOHNSON, Julian LEWIS, David Owen MORGAN, Martin C. RAFF, Keith ROBERTS and Peter WALTER. Molecular biology of the cell. Edited by John H. Wilson - Tim Hunt. Sixth edition. New York, NY: Garland Science, 2014, xxxiv, 134. ISBN 9780815344322. info
• SAMBROOK, Joseph and David W. RUSSELL. Molecular cloning : a laboratory manual. 3rd ed. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press, 2001, 1 sv. ISBN 0879695765. info

Teaching methods

praktická výuka

Assessment methods

Test.

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

• Enrolment Statistics (recent)
  
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