Bi9080 Technical processes in biotechnology
Faculty of ScienceAutumn 2011
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc. - Timetable
- Tue 10:00–11:50 Bpt,01013
- Prerequisites
- The course is destined for students interested in microbiology and biotechnology. Enrolling in the course requires as a prerequisite the examination in the course Molecular Biotechnology (Bi7430)
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)
- Course objectives
- At the end of this course, the students will be able to understand of the principes of methods and processes used at the isolation of biotechnological products. Students will demonstrate knowledge of the use of methods for the solving of practical probleme.
- Syllabus
- Stechiometry of microbial growth. Material balances.
- Fluids properties. Fluid transfer in piping.
- Bioreactors support systems and processes. Systems with immobilized cells and enzymes.
- Influence of solvents on enzyme activities.
- Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation.
- Precipitation. Salting out. Denaturation and renaturation of proteins.
- Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design.
- . Membrane separation processes. Large scale chromatographic purification of proteins.
- Drying. Storage and stability of proteins. Validation of production quality.
- Literature
- 1. Ledersen B.K., DElia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
- Teaching methods
- The teaching methods make use of presentations on stencils. For the explanation of problems are used practical examples and calculations.
- Assessment methods
- The course is closed with an oral exam. The student answers 3 questions in the exam. The overall result of the exam is evaluated by the examiner.
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Bi9080 Technical processes in biotechnology
Faculty of ScienceAutumn 2010
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc. - Timetable
- Tue 12:00–13:50 Bpt,01013
- Prerequisites
- The course is destined for students interested in microbiology and biotechnology. Enrolling in the course requires as a prerequisite the examination in the course Molecular Biotechnology (Bi7430)
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Course objectives
- At the end of this course, the students will be able to understand of the principes of methods and processes used at the isolation of biotechnological products. Students will demonstrate knowledge of the use of methods for the solving of practical probleme.
- Syllabus
- Stechiometry of microbial growth. Material balances.
- Fluids properties. Fluid transfer in piping.
- Bioreactors support systems and processes. Systems with immobilized cells and enzymes.
- Influence of solvents on enzyme activities.
- Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation.
- Precipitation. Salting out. Denaturation and renaturation of proteins.
- Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design.
- . Membrane separation processes. Large scale chromatographic purification of proteins.
- Drying. Storage and stability of proteins. Validation of production quality.
- Literature
- 1. Ledersen B.K., DElia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
- Teaching methods
- The teaching methods make use of presentations on stencils. For the explanation of problems are used practical examples and calculations.
- Assessment methods
- The course is closed with an oral exam. The student answers 3 questions in the exam. The overall result of the exam is evaluated by the examiner.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
Bi9080 Technical processes in biotechnology
Faculty of ScienceAutumn 2009
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
- Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc. - Timetable
- Mon 12:00–13:50 Bpt,01013
- Prerequisites
- The course is destined for students interested in microbiology and biotechnology. Enrolling in the course requires as a prerequisite the examination in the course Molecular Biotechnology (Bi7430)
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Course objectives
- At the end of this course, the students will be able to understand of the principes of methods and processes used at the isolation of biotechnological products. Students will demonstrate knowledge of the use of methods for the solving of practical probleme.
- Syllabus
- Stechiometry of microbial growth. Material balances.
- Fluids properties. Fluid transfer in piping.
- Bioreactors support systems and processes. Systems with immobilized cells and enzymes.
- Influence of solvents on enzyme activities.
- Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation.
- Precipitation. Salting out. Denaturation and renaturation of proteins.
- Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design.
- . Membrane separation processes. Large scale chromatographic purification of proteins.
- Drying. Storage and stability of proteins. Validation of production quality.
- Literature
- 1. Ledersen B.K., DElia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
- Teaching methods
- The teaching methods make use of presentations on stencils. For the explanation of problems are used practical examples and calculations.
- Assessment methods
- The course is closed with an oral exam. The student answers 3 questions in the exam. The overall result of the exam is evaluated by the examiner.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
Bi9080 Technical processes in biotechnology
Faculty of ScienceAutumn 2008
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
- Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc. - Timetable
- Tue 14:00–15:50 Bpt,01013
- Prerequisites (in Czech)
- Výuka je určena studujícím oboru mikrobiologie a dalším zájemcům z jiných oborů. Předpokladem je zkouška z předmětu Úvod do biotechnologie (B7430).
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Course objectives
- Material balances. Fluids properties. Bioreactors support systems and processes. Systems with immobilized cells and enzymes. Influence of solvents on enzyme activities. Methods of product isolation. Filtration. Centrifugation. Precipitation. Salting out. Denaturation and renaturation of proteins. Liquid-liquid extraction. Membrane separation processes. Large scale chromatographic purification. Drying. Storage and stability of proteins. Main objectives can be summarized as follows: understand to the principes of methods and processes used at the isolation of biotechnological products
- Syllabus
- Stechiometry of microbial growth. Material balances.
- Fluids properties. Fluid transfer in piping.
- Bioreactors support systems and processes. Systems with immobilized cells and enzymes.
- Influence of solvents on enzyme activities.
- Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation.
- Precipitation. Salting out. Denaturation and renaturation of proteins.
- Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design.
- . Membrane separation processes. Large scale chromatographic purification of proteins.
- Drying. Storage and stability of proteins. Validation of production quality.
- Literature
- 1. Ledersen B.K., DElia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
- Assessment methods
- Oral exam.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
Bi9080 Technical processes in biotechnology
Faculty of ScienceAutumn 2007
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
- Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc. - Timetable
- Thu 16:00–17:50 Bpt,01013
- Prerequisites (in Czech)
- Výuka je určena studujícím oboru mikrobiologie a dalším zájemcům z jiných oborů. Předpokladem je zkouška z předmětu Úvod do biotechnologie (B7430).
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Course objectives
- Stoichiometry of microbial growth. Material balances. Fluids properties. Fluid transfer in piping. Bioreactors support systems and processes. Systems with immobilized cells and enzymes. Influence of solvents on enzyme activities. Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation. Precipitation. Salting out. Denaturation and renaturation of proteins. Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design. Destilation. Membrane separation processes. Large scale chromatographic purification of proteins. Drying. Storage and stability of proteins. Validation of production quality.
- Literature
- 1. Ledersen B.K., DElia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
- Assessment methods (in Czech)
- Výuka je ukončena zkouškou. Cvičení je ukončeno zápočtem.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
Bi9080 Technical processes in biotechnology
Faculty of ScienceAutumn 2006
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
- Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc. - Timetable
- Mon 9:00–10:50 Bpt,01013
- Prerequisites (in Czech)
- Výuka je určena studujícím oboru mikrobiologie a dalším zájemcům z jiných oborů. Předpokladem je zkouška z předmětu Úvod do biotechnologie (B7430).
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Course objectives
- Stoichiometry of microbial growth. Material balances. Fluids properties. Fluid transfer in piping. Bioreactors support systems and processes. Systems with immobilized cells and enzymes. Influence of solvents on enzyme activities. Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation. Precipitation. Salting out. Denaturation and renaturation of proteins. Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design. Destilation. Membrane separation processes. Large scale chromatographic purification of proteins. Drying. Storage and stability of proteins. Validation of production quality.
- Literature
- 1. Ledersen B.K., DElia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
- Assessment methods (in Czech)
- Výuka je ukončena zkouškou. Cvičení je ukončeno zápočtem.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
Bi9080 Technical processes in biotechnology
Faculty of ScienceAutumn 2005
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
- Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc. - Timetable
- Wed 11:00–12:50 Bpt,01013
- Prerequisites (in Czech)
- Výuka je určena studujícím oboru mikrobiologie a dalším zájemcům z jiných oborů. Předpokladem je zkouška z předmětu Úvod do biotechnologie (B7430).
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Course objectives
- Stoichiometry of microbial growth. Material balances. Fluids properties. Fluid transfer in piping. Bioreactors support systems and processes. Systems with immobilized cells and enzymes. Influence of solvents on enzyme activities. Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation. Precipitation. Salting out. Denaturation and renaturation of proteins. Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design. Destilation. Membrane separation processes. Large scale chromatographic purification of proteins. Drying. Storage and stability of proteins. Validation of production quality.
- Literature
- 1. Ledersen B.K., DElia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
- Assessment methods (in Czech)
- Výuka je ukončena zkouškou. Cvičení je ukončeno zápočtem.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
Bi9080 Technical processes in biotechnology
Faculty of ScienceAutumn 2004
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
- Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc. - Timetable
- Thu 16:00–17:50 Bpt,01013
- Prerequisites (in Czech)
- Výuka je určena studujícím oboru mikrobiologie a dalším zájemcům z jiných oborů. Předpokladem je zkouška z předmětu Úvod do biotechnologie (B7430).
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Course objectives
- Stoichiometry of microbial growth. Material balances. Fluids properties. Fluid transfer in piping. Similarity of systems and processes. Diffusion. Bioreactors support systems and processes. Systems with immobilized cells and enzymes. Influence of solvents on enzyme activities. Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation. Precipitation. Salting out. Denaturation and renaturation of proteins. Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design. Destilation. Membrane separation processes. Large scale chromatographic purification of proteins. Drying. Storage and stability of proteins. Validation of production quality.
- Literature
- 1. Ledersen B.K., D´Elia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
- Assessment methods (in Czech)
- Výuka je ukončena zkouškou. Cvičení je ukončeno zápočtem.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
Bi9080 Technical processes in biotechnology
Faculty of ScienceAutumn 2003
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
- Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc. - Prerequisites (in Czech)
- Výuka je určena studujícím oboru mikrobiologie a dalším zájemcům z jiných oborů. Předpokladem je zkouška z předmětu Úvod do biotechnologie (B7430).
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Course objectives
- Stoichiometry of microbial growth. Material balances. Fluids properties. Fluid transfer in piping. Similarity of systems and processes. Diffusion. Bioreactors support systems and processes. Systems with immobilized cells and enzymes. Influence of solvents on enzyme activities. Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation. Precipitation. Salting out. Denaturation and renaturation of proteins. Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design. Destilation. Membrane separation processes. Large scale chromatographic purification of proteins. Drying. Storage and stability of proteins. Validation of production quality.
- Literature
- 1. Ledersen B.K., D´Elia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
- Assessment methods (in Czech)
- Výuka je ukončena zkouškou. Cvičení je ukončeno zápočtem.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Bi9080 Technical processes in biotechnology
Faculty of ScienceAutumn 2002
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
- Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc. - Prerequisites (in Czech)
- Výuka je určena studujícím oboru mikrobiologie a dalším zájemcům z jiných oborů. Předpokladem je zkouška z předmětu Úvod do biotechnologie (B7430).
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Course objectives
- Stoichiometry of microbial growth. Material balances. Fluids properties. Fluid transfer in piping. Similarity of systems and processes. Diffusion. Bioreactors support systems and processes. Systems with immobilized cells and enzymes. Influence of solvents on enzyme activities. Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation. Precipitation. Salting out. Denaturation and renaturation of proteins. Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design. Destilation. Membrane separation processes. Large scale chromatographic purification of proteins. Drying. Storage and stability of proteins. Validation of production quality.
- Literature
- 1. Ledersen B.K., D´Elia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
- Assessment methods (in Czech)
- Výuka je ukončena zkouškou. Cvičení je ukončeno zápočtem.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Bi9080 Technical processes in biotechnology
Faculty of ScienceAutumn 2024
The course is not taught in Autumn 2024
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites
- The course is destined for students interested in microbiology and biotechnology. Enrolling in the course requires as a prerequisite the examination in the course Molecular Biotechnology (Bi7430)
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)
- Course objectives
- At the end of this course, the students will be able to understand of the principes of methods and processes used at the isolation of biotechnological products. Students will demonstrate knowledge of the use of methods for the solving of practical probleme.
- Syllabus
- Stechiometry of microbial growth. Material balances.
- Fluids properties. Fluid transfer in piping.
- Bioreactors support systems and processes. Systems with immobilized cells and enzymes.
- Influence of solvents on enzyme activities.
- Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation.
- Precipitation. Salting out. Denaturation and renaturation of proteins.
- Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design.
- . Membrane separation processes. Large scale chromatographic purification of proteins.
- Drying. Storage and stability of proteins. Validation of production quality.
- Literature
- 1. Ledersen B.K., DElia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
- Teaching methods
- The teaching methods make use of presentations on stencils. For the explanation of problems are used practical examples and calculations.
- Assessment methods
- The course is closed with an oral exam. The student answers 3 questions in the exam. The overall result of the exam is evaluated by the examiner.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Bi9080 Technical processes in biotechnology
Faculty of ScienceAutumn 2023
The course is not taught in Autumn 2023
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites
- The course is destined for students interested in microbiology and biotechnology. Enrolling in the course requires as a prerequisite the examination in the course Molecular Biotechnology (Bi7430)
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)
- Course objectives
- At the end of this course, the students will be able to understand of the principes of methods and processes used at the isolation of biotechnological products. Students will demonstrate knowledge of the use of methods for the solving of practical probleme.
- Syllabus
- Stechiometry of microbial growth. Material balances.
- Fluids properties. Fluid transfer in piping.
- Bioreactors support systems and processes. Systems with immobilized cells and enzymes.
- Influence of solvents on enzyme activities.
- Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation.
- Precipitation. Salting out. Denaturation and renaturation of proteins.
- Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design.
- . Membrane separation processes. Large scale chromatographic purification of proteins.
- Drying. Storage and stability of proteins. Validation of production quality.
- Literature
- 1. Ledersen B.K., DElia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
- Teaching methods
- The teaching methods make use of presentations on stencils. For the explanation of problems are used practical examples and calculations.
- Assessment methods
- The course is closed with an oral exam. The student answers 3 questions in the exam. The overall result of the exam is evaluated by the examiner.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Bi9080 Technical processes in biotechnology
Faculty of ScienceAutumn 2022
The course is not taught in Autumn 2022
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites
- The course is destined for students interested in microbiology and biotechnology. Enrolling in the course requires as a prerequisite the examination in the course Molecular Biotechnology (Bi7430)
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)
- Course objectives
- At the end of this course, the students will be able to understand of the principes of methods and processes used at the isolation of biotechnological products. Students will demonstrate knowledge of the use of methods for the solving of practical probleme.
- Syllabus
- Stechiometry of microbial growth. Material balances.
- Fluids properties. Fluid transfer in piping.
- Bioreactors support systems and processes. Systems with immobilized cells and enzymes.
- Influence of solvents on enzyme activities.
- Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation.
- Precipitation. Salting out. Denaturation and renaturation of proteins.
- Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design.
- . Membrane separation processes. Large scale chromatographic purification of proteins.
- Drying. Storage and stability of proteins. Validation of production quality.
- Literature
- 1. Ledersen B.K., DElia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
- Teaching methods
- The teaching methods make use of presentations on stencils. For the explanation of problems are used practical examples and calculations.
- Assessment methods
- The course is closed with an oral exam. The student answers 3 questions in the exam. The overall result of the exam is evaluated by the examiner.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Bi9080 Technical processes in biotechnology
Faculty of Scienceautumn 2021
The course is not taught in autumn 2021
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites
- The course is destined for students interested in microbiology and biotechnology. Enrolling in the course requires as a prerequisite the examination in the course Molecular Biotechnology (Bi7430)
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)
- Course objectives
- At the end of this course, the students will be able to understand of the principes of methods and processes used at the isolation of biotechnological products. Students will demonstrate knowledge of the use of methods for the solving of practical probleme.
- Syllabus
- Stechiometry of microbial growth. Material balances.
- Fluids properties. Fluid transfer in piping.
- Bioreactors support systems and processes. Systems with immobilized cells and enzymes.
- Influence of solvents on enzyme activities.
- Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation.
- Precipitation. Salting out. Denaturation and renaturation of proteins.
- Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design.
- . Membrane separation processes. Large scale chromatographic purification of proteins.
- Drying. Storage and stability of proteins. Validation of production quality.
- Literature
- 1. Ledersen B.K., DElia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
- Teaching methods
- The teaching methods make use of presentations on stencils. For the explanation of problems are used practical examples and calculations.
- Assessment methods
- The course is closed with an oral exam. The student answers 3 questions in the exam. The overall result of the exam is evaluated by the examiner.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Bi9080 Technical processes in biotechnology
Faculty of ScienceAutumn 2020
The course is not taught in Autumn 2020
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites
- The course is destined for students interested in microbiology and biotechnology. Enrolling in the course requires as a prerequisite the examination in the course Molecular Biotechnology (Bi7430)
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)
- Course objectives
- At the end of this course, the students will be able to understand of the principes of methods and processes used at the isolation of biotechnological products. Students will demonstrate knowledge of the use of methods for the solving of practical probleme.
- Syllabus
- Stechiometry of microbial growth. Material balances.
- Fluids properties. Fluid transfer in piping.
- Bioreactors support systems and processes. Systems with immobilized cells and enzymes.
- Influence of solvents on enzyme activities.
- Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation.
- Precipitation. Salting out. Denaturation and renaturation of proteins.
- Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design.
- . Membrane separation processes. Large scale chromatographic purification of proteins.
- Drying. Storage and stability of proteins. Validation of production quality.
- Literature
- 1. Ledersen B.K., DElia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
- Teaching methods
- The teaching methods make use of presentations on stencils. For the explanation of problems are used practical examples and calculations.
- Assessment methods
- The course is closed with an oral exam. The student answers 3 questions in the exam. The overall result of the exam is evaluated by the examiner.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Bi9080 Technical processes in biotechnology
Faculty of ScienceAutumn 2019
The course is not taught in Autumn 2019
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites
- The course is destined for students interested in microbiology and biotechnology. Enrolling in the course requires as a prerequisite the examination in the course Molecular Biotechnology (Bi7430)
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)
- Course objectives
- At the end of this course, the students will be able to understand of the principes of methods and processes used at the isolation of biotechnological products. Students will demonstrate knowledge of the use of methods for the solving of practical probleme.
- Syllabus
- Stechiometry of microbial growth. Material balances.
- Fluids properties. Fluid transfer in piping.
- Bioreactors support systems and processes. Systems with immobilized cells and enzymes.
- Influence of solvents on enzyme activities.
- Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation.
- Precipitation. Salting out. Denaturation and renaturation of proteins.
- Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design.
- . Membrane separation processes. Large scale chromatographic purification of proteins.
- Drying. Storage and stability of proteins. Validation of production quality.
- Literature
- 1. Ledersen B.K., DElia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
- Teaching methods
- The teaching methods make use of presentations on stencils. For the explanation of problems are used practical examples and calculations.
- Assessment methods
- The course is closed with an oral exam. The student answers 3 questions in the exam. The overall result of the exam is evaluated by the examiner.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Bi9080 Technical processes in biotechnology
Faculty of ScienceAutumn 2018
The course is not taught in Autumn 2018
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites
- The course is destined for students interested in microbiology and biotechnology. Enrolling in the course requires as a prerequisite the examination in the course Molecular Biotechnology (Bi7430)
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)
- Course objectives
- At the end of this course, the students will be able to understand of the principes of methods and processes used at the isolation of biotechnological products. Students will demonstrate knowledge of the use of methods for the solving of practical probleme.
- Syllabus
- Stechiometry of microbial growth. Material balances.
- Fluids properties. Fluid transfer in piping.
- Bioreactors support systems and processes. Systems with immobilized cells and enzymes.
- Influence of solvents on enzyme activities.
- Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation.
- Precipitation. Salting out. Denaturation and renaturation of proteins.
- Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design.
- . Membrane separation processes. Large scale chromatographic purification of proteins.
- Drying. Storage and stability of proteins. Validation of production quality.
- Literature
- 1. Ledersen B.K., DElia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
- Teaching methods
- The teaching methods make use of presentations on stencils. For the explanation of problems are used practical examples and calculations.
- Assessment methods
- The course is closed with an oral exam. The student answers 3 questions in the exam. The overall result of the exam is evaluated by the examiner.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Bi9080 Technical processes in biotechnology
Faculty of Scienceautumn 2017
The course is not taught in autumn 2017
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites
- The course is destined for students interested in microbiology and biotechnology. Enrolling in the course requires as a prerequisite the examination in the course Molecular Biotechnology (Bi7430)
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)
- Course objectives
- At the end of this course, the students will be able to understand of the principes of methods and processes used at the isolation of biotechnological products. Students will demonstrate knowledge of the use of methods for the solving of practical probleme.
- Syllabus
- Stechiometry of microbial growth. Material balances.
- Fluids properties. Fluid transfer in piping.
- Bioreactors support systems and processes. Systems with immobilized cells and enzymes.
- Influence of solvents on enzyme activities.
- Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation.
- Precipitation. Salting out. Denaturation and renaturation of proteins.
- Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design.
- . Membrane separation processes. Large scale chromatographic purification of proteins.
- Drying. Storage and stability of proteins. Validation of production quality.
- Literature
- 1. Ledersen B.K., DElia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
- Teaching methods
- The teaching methods make use of presentations on stencils. For the explanation of problems are used practical examples and calculations.
- Assessment methods
- The course is closed with an oral exam. The student answers 3 questions in the exam. The overall result of the exam is evaluated by the examiner.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Bi9080 Technical processes in biotechnology
Faculty of ScienceAutumn 2016
The course is not taught in Autumn 2016
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites
- The course is destined for students interested in microbiology and biotechnology. Enrolling in the course requires as a prerequisite the examination in the course Molecular Biotechnology (Bi7430)
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)
- Course objectives
- At the end of this course, the students will be able to understand of the principes of methods and processes used at the isolation of biotechnological products. Students will demonstrate knowledge of the use of methods for the solving of practical probleme.
- Syllabus
- Stechiometry of microbial growth. Material balances.
- Fluids properties. Fluid transfer in piping.
- Bioreactors support systems and processes. Systems with immobilized cells and enzymes.
- Influence of solvents on enzyme activities.
- Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation.
- Precipitation. Salting out. Denaturation and renaturation of proteins.
- Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design.
- . Membrane separation processes. Large scale chromatographic purification of proteins.
- Drying. Storage and stability of proteins. Validation of production quality.
- Literature
- 1. Ledersen B.K., DElia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
- Teaching methods
- The teaching methods make use of presentations on stencils. For the explanation of problems are used practical examples and calculations.
- Assessment methods
- The course is closed with an oral exam. The student answers 3 questions in the exam. The overall result of the exam is evaluated by the examiner.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Bi9080 Technical processes in biotechnology
Faculty of ScienceAutumn 2015
The course is not taught in Autumn 2015
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites
- The course is destined for students interested in microbiology and biotechnology. Enrolling in the course requires as a prerequisite the examination in the course Molecular Biotechnology (Bi7430)
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)
- Course objectives
- At the end of this course, the students will be able to understand of the principes of methods and processes used at the isolation of biotechnological products. Students will demonstrate knowledge of the use of methods for the solving of practical probleme.
- Syllabus
- Stechiometry of microbial growth. Material balances.
- Fluids properties. Fluid transfer in piping.
- Bioreactors support systems and processes. Systems with immobilized cells and enzymes.
- Influence of solvents on enzyme activities.
- Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation.
- Precipitation. Salting out. Denaturation and renaturation of proteins.
- Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design.
- . Membrane separation processes. Large scale chromatographic purification of proteins.
- Drying. Storage and stability of proteins. Validation of production quality.
- Literature
- 1. Ledersen B.K., DElia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
- Teaching methods
- The teaching methods make use of presentations on stencils. For the explanation of problems are used practical examples and calculations.
- Assessment methods
- The course is closed with an oral exam. The student answers 3 questions in the exam. The overall result of the exam is evaluated by the examiner.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Bi9080 Technical processes in biotechnology
Faculty of ScienceAutumn 2014
The course is not taught in Autumn 2014
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites
- The course is destined for students interested in microbiology and biotechnology. Enrolling in the course requires as a prerequisite the examination in the course Molecular Biotechnology (Bi7430)
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)
- Course objectives
- At the end of this course, the students will be able to understand of the principes of methods and processes used at the isolation of biotechnological products. Students will demonstrate knowledge of the use of methods for the solving of practical probleme.
- Syllabus
- Stechiometry of microbial growth. Material balances.
- Fluids properties. Fluid transfer in piping.
- Bioreactors support systems and processes. Systems with immobilized cells and enzymes.
- Influence of solvents on enzyme activities.
- Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation.
- Precipitation. Salting out. Denaturation and renaturation of proteins.
- Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design.
- . Membrane separation processes. Large scale chromatographic purification of proteins.
- Drying. Storage and stability of proteins. Validation of production quality.
- Literature
- 1. Ledersen B.K., DElia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
- Teaching methods
- The teaching methods make use of presentations on stencils. For the explanation of problems are used practical examples and calculations.
- Assessment methods
- The course is closed with an oral exam. The student answers 3 questions in the exam. The overall result of the exam is evaluated by the examiner.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Bi9080 Technical processes in biotechnology
Faculty of ScienceAutumn 2013
The course is not taught in Autumn 2013
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites
- The course is destined for students interested in microbiology and biotechnology. Enrolling in the course requires as a prerequisite the examination in the course Molecular Biotechnology (Bi7430)
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)
- Course objectives
- At the end of this course, the students will be able to understand of the principes of methods and processes used at the isolation of biotechnological products. Students will demonstrate knowledge of the use of methods for the solving of practical probleme.
- Syllabus
- Stechiometry of microbial growth. Material balances.
- Fluids properties. Fluid transfer in piping.
- Bioreactors support systems and processes. Systems with immobilized cells and enzymes.
- Influence of solvents on enzyme activities.
- Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation.
- Precipitation. Salting out. Denaturation and renaturation of proteins.
- Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design.
- . Membrane separation processes. Large scale chromatographic purification of proteins.
- Drying. Storage and stability of proteins. Validation of production quality.
- Literature
- 1. Ledersen B.K., DElia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
- Teaching methods
- The teaching methods make use of presentations on stencils. For the explanation of problems are used practical examples and calculations.
- Assessment methods
- The course is closed with an oral exam. The student answers 3 questions in the exam. The overall result of the exam is evaluated by the examiner.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Bi9080 Technical processes in biotechnology
Faculty of ScienceAutumn 2012
The course is not taught in Autumn 2012
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites
- The course is destined for students interested in microbiology and biotechnology. Enrolling in the course requires as a prerequisite the examination in the course Molecular Biotechnology (Bi7430)
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)
- Course objectives
- At the end of this course, the students will be able to understand of the principes of methods and processes used at the isolation of biotechnological products. Students will demonstrate knowledge of the use of methods for the solving of practical probleme.
- Syllabus
- Stechiometry of microbial growth. Material balances.
- Fluids properties. Fluid transfer in piping.
- Bioreactors support systems and processes. Systems with immobilized cells and enzymes.
- Influence of solvents on enzyme activities.
- Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation.
- Precipitation. Salting out. Denaturation and renaturation of proteins.
- Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design.
- . Membrane separation processes. Large scale chromatographic purification of proteins.
- Drying. Storage and stability of proteins. Validation of production quality.
- Literature
- 1. Ledersen B.K., DElia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
- Teaching methods
- The teaching methods make use of presentations on stencils. For the explanation of problems are used practical examples and calculations.
- Assessment methods
- The course is closed with an oral exam. The student answers 3 questions in the exam. The overall result of the exam is evaluated by the examiner.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Bi9080 Technical processes in biotechnology
Faculty of ScienceAutumn 2011 - acreditation
The information about the term Autumn 2011 - acreditation is not made public
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc. - Prerequisites
- The course is destined for students interested in microbiology and biotechnology. Enrolling in the course requires as a prerequisite the examination in the course Molecular Biotechnology (Bi7430)
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Course objectives
- At the end of this course, the students will be able to understand of the principes of methods and processes used at the isolation of biotechnological products. Students will demonstrate knowledge of the use of methods for the solving of practical probleme.
- Syllabus
- Stechiometry of microbial growth. Material balances.
- Fluids properties. Fluid transfer in piping.
- Bioreactors support systems and processes. Systems with immobilized cells and enzymes.
- Influence of solvents on enzyme activities.
- Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation.
- Precipitation. Salting out. Denaturation and renaturation of proteins.
- Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design.
- . Membrane separation processes. Large scale chromatographic purification of proteins.
- Drying. Storage and stability of proteins. Validation of production quality.
- Literature
- 1. Ledersen B.K., DElia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
- Teaching methods
- The teaching methods make use of presentations on stencils. For the explanation of problems are used practical examples and calculations.
- Assessment methods
- The course is closed with an oral exam. The student answers 3 questions in the exam. The overall result of the exam is evaluated by the examiner.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Bi9080 Technical processes in biotechnology
Faculty of ScienceAutumn 2010 - only for the accreditation
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc. - Prerequisites
- The course is destined for students interested in microbiology and biotechnology. Enrolling in the course requires as a prerequisite the examination in the course Molecular Biotechnology (Bi7430)
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Course objectives
- At the end of this course, the students will be able to understand of the principes of methods and processes used at the isolation of biotechnological products. Students will demonstrate knowledge of the use of methods for the solving of practical probleme.
- Syllabus
- Stechiometry of microbial growth. Material balances.
- Fluids properties. Fluid transfer in piping.
- Bioreactors support systems and processes. Systems with immobilized cells and enzymes.
- Influence of solvents on enzyme activities.
- Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation.
- Precipitation. Salting out. Denaturation and renaturation of proteins.
- Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design.
- . Membrane separation processes. Large scale chromatographic purification of proteins.
- Drying. Storage and stability of proteins. Validation of production quality.
- Literature
- 1. Ledersen B.K., DElia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
- Teaching methods
- The teaching methods make use of presentations on stencils. For the explanation of problems are used practical examples and calculations.
- Assessment methods
- The course is closed with an oral exam. The student answers 3 questions in the exam. The overall result of the exam is evaluated by the examiner.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Bi9080 Technical processes in biotechnology
Faculty of ScienceAutumn 2007 - for the purpose of the accreditation
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
- Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Ing. Bohuslav Rittich, CSc. - Prerequisites (in Czech)
- Výuka je určena studujícím oboru mikrobiologie a dalším zájemcům z jiných oborů. Předpokladem je zkouška z předmětu Úvod do biotechnologie (B7430).
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Course objectives
- Stoichiometry of microbial growth. Material balances. Fluids properties. Fluid transfer in piping. Bioreactors support systems and processes. Systems with immobilized cells and enzymes. Influence of solvents on enzyme activities. Methods of product isolation. Mechanical, chemical and enzymatic cell disruption. Filtration. Centrifugation. Precipitation. Salting out. Denaturation and renaturation of proteins. Liquid-liquid extraction. Aqueous two-phase systems. Types of extractors and their design. Destilation. Membrane separation processes. Large scale chromatographic purification of proteins. Drying. Storage and stability of proteins. Validation of production quality.
- Literature
- 1. Ledersen B.K., DElia N.A., Nelson K.L. Bioprocess engineering: systems, equipment and facilities. John Wiley, New York 1994. 2. Weatherley L.R. Engineering Processes for Bioseparations. Butterworth-Heinemann, Oxford 1994.
- Assessment methods (in Czech)
- Výuka je ukončena zkouškou. Cvičení je ukončeno zápočtem.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
- Enrolment Statistics (recent)