C3002 Nanobiotechnology

Faculty of Science
Spring 2022
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Taught in person.
Teacher(s)
Mgr. Zdeněk Farka, Ph.D. (lecturer)
Mgr. Jan Přibyl, Ph.D. (lecturer)
doc. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
doc. RNDr. Petr Skládal, CSc.
Department of Biochemistry - Chemistry Section - Faculty of Science
Supplier department: Department of Biochemistry - Chemistry Section - Faculty of Science
Prerequisites
Basic knowledge of biochemistry and biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Introduction of basic principles of nanobiotechnology - applications of nanometer sized biological objects in technology, preparation, characterization and bioconjugation of nanoparticles, and studies of biomolecules, cells and other biological objects with the help of scanning probe microscopies.
Learning outcomes
Student will learn basic principles of nanobiotechnology - applications of nanometer sized biological objects in technology, preparation, characterization and bioconjugation of nanoparticles, and studies of biomolecules, cells and other biological objects with the help of scanning probe microscopies.
Syllabus
  • 1. Introduction. Science of Nano. What is nanobiotechnology. 2. Nanostructures. Carbon nanotubes, semiconductor nanoparticles - quantum dots. Metal-based nanostructures - nanowires and bioelectronics. Gold nanoparticles (nanorods, nanocages, nanoshells). Magnetic nanoparticles. Polymer nanostructures (dendrimers). Protein-based nanostructures - nanomotors from microbes and mammalian cells (myosin). Nanomachines based on nucleic acids. 3. Experimental technichues. Scanning probe microscopies (STM, AFM, SNOM, SECM, ...). Physical principles, basic and advanced measuring modes. Imaging of bioobjects - from atoms, molecules to cells and tissues. Combined techniques with inverted optical and fluorescence microscopes. Raman imaging. Biointeractions at the molecular level. 4. Self-assembling techniques. Separation, characterization and modification of nanoparticles. From natural to artificial structures. Nanolithography and nanomanipulations. Nanoparticles for biological labeling and cellular imaging. Nanobiosensors and nanobioanalytical systems. Microfluidics, cell sorting and lab-on-a-chip. Biochips and sensing arrays, nanodeposition of biomolecules. 5. Medical applications. Cytotoxicity of nanoparticles. Nanostructures in drug discovery, delivery and controlled release. Nanostructures in cancer research. Nanotechnology for tissue engineering and regenerative therapy. 6. Nanobiotechnology in commercial examples. Perspectives and conclusions.
Literature
  • www.nanobio.cz
Teaching methods
lecture course, accompanied by participation at demonstration experiments, visit in research laboratory
Assessment methods
Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium
Language of instruction
English
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Teacher's information
http://www.nanobio.cz

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