C4220 Biochemistry - laboratory course

Faculty of Science
Spring 2006
Extent and Intensity
0/0/7. 7 credit(s). Type of Completion: graded credit.
Teacher(s)
RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
doc. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Ing. Martin Mandl, CSc. (seminar tutor)
prof. RNDr. Vladimír Mikeš, CSc. (seminar tutor)
doc. RNDr. Stanislav Pavelka, CSc. (seminar tutor)
doc. RNDr. Petr Skládal, CSc. (seminar tutor)
Guaranteed by
RNDr. Pavla Boublíková, CSc.
Department of Biochemistry - Chemistry Section - Faculty of Science
Timetable of Seminar Groups
C4220/01: Fri 7:00–12:50 C02/113, T. Kašparovský
C4220/02: Thu 7:00–12:50 C02/113, P. Bouchal
C4220/03: Wed 14:00–19:50 C02/113, O. Janiczek
C4220/04: Tue 14:00–19:50 C02/113, P. Boublíková
C4220/05: Wed 7:00–12:50 C02/113, P. Boublíková
Prerequisites
C3181 Biochemistry I
Basic knowledge of Biochemistry I
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
there are 28 fields of study the course is directly associated with, display
Course objectives
The objective of the laboratory course are studies of basic properties of compounds important for biochemistry, and methodological approaches in the study and analytical application of enzymes. Content of the first part includes qualitative and quantitative determination of carbohydrates, amino acids, proteins and nucleic acids. The second part is oriented to enzyme kinetics and further studies on properties of enzymes.
Syllabus
  • Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Heat dependence of enzyme reaction. pH effect on enzyme reaction. Enzyme kinetics. Determination of enzyme activity. Inhibition of enzymes. Enzymatic determination of metabolites, substrate specifity of enzymes.Imobilization of enzymes.Respiratory chain chain of aerobic organisms.
Literature
  • Návody ke cvičením - viz Studijní materiály
  • VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995. S. II-XIV,. ISBN 80-85605-44-9. info
  • VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999. 1 sv. ISBN 0471586501. info
Assessment methods (in Czech)
První cvičení je věnováno poučení o bezpečnosti práce v biochemické laboratoři. Studenti rovněž píší vstupní test, jehož úspěšné absolvování je podmínkou účasti ve cvičení. Před každým dalším cvičením se písemným testem ověřuje příprava studenta na danou úlohu.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught each semester.
General note: Vyučují učitelé katedry biochemie.
Information on course enrolment limitations: V podzimním semestru se vypisuje pouze jedna skupina pro 12 studentů chemických oborů jako náhrada za JS
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021.
  • Enrolment Statistics (Spring 2006, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2006/C4220