PřF:C4830 Inst. Methods in Biochemistry - Course Information
C4830 Instrumental Methods in BiochemistryFaculty of Science
- Extent and Intensity
- 2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
- prof. RNDr. Zdeněk Glatz, CSc. (lecturer)
- Guaranteed by
- prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry - Chemistry Section - Faculty of Science
Supplier department: Department of Biochemistry - Chemistry Section - Faculty of Science
- C3181 Biochemistry I || C3580 Biochemistry
Basic knowledge of general biochemistry in range of the lectures Biochemistry I (C3181) or Biochemistry(C3580).
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
- fields of study / plans the course is directly associated with
- Chemoinformatics and Bioinformatics (programme PřF, B-BCH)
- Course objectives
- The students should have the basic knowledge of the separation methods in biochemistry or molecular biology at the end of the course. Contents of the first part include the basic manipulation with biological samples - extraction, clarification, precipitation, ultrafiltration and lyophilisation. Second part is oriented onto chromatography methods. Last part is dedicated in electromigration methods.
- Learning outcomes
- At the end of this course the students should not only theoretically understand but be able practically apply the methods of disintegration of animal, vegetal and microbial tissues, methods of isolation, purification of proteins, nucleic acids and other biopolymers, methods for determination their purity and molecular properties. They should be able to apply these knowledges in their scientific research working on bachelor, master and PhD theses and finally during their following employment as well.
- 1.Introduction. Basic Preparation. Purification Strategy. 2.Clarification and Extraction. Centrifugation. 3.Phase Separation. Precipitation and Selective Denaturation. Membrane Separation. 4.Ultrafiltration and Lyophilisation. Laboratory Water Preparation. 5.Chromatography. Basic Principles. 6.Adsorption and Partition Chromatography. 7.Ion-exchange Chromatography. Chromatofocusing. 8.Reverse Phase and Ion Pair Chromatography. Hydrophobic Chromatography 9.Gel Permeation Chromatography. 10.Affinity Chromatography. 11.Gas Chromatography. 12.Electromigration Metods. Basic Principles. Free and Zone Electrophoresis. 13.Isoelectric Focusing. 14.Isotachophoresis.
- ANZENBACHER, Pavel and Jan KOVÁŘ. Metody chemického výzkumu pro biochemiky. 1. vyd. Praha: Ministerstvo školství ČSR, 1986. 199 s. info
- SNYDER, Lloyd R., Joseph Jack KIRKLAND and Joseph L. GLAJCH. Practical HPLC method development [Snyder, 1997]. 2nd ed. New York: John Wiley & Sons, 1997. xxvi, 765. ISBN 0-471-00703-X. info
- Teaching methods
- Lectures with the demonstrations of the taught topic by means of educational videos and programs that are available for the students on web page of this course.
- Assessment methods
- The final exam is in the form of written test, which contains questions from the sample extraction, centrifugation, electromigration and chromatographic methods. The time for the test is one hour, the grades are A to F on the basis of each question classification.
- Language of instruction
- Follow-Up Courses
- Further comments (probably available only in Czech)
- The course can also be completed outside the examination period.
The course is taught once in two years.
Information on the per-term frequency of the course: (sudé roky).
The course is taught: every week.
- Listed among pre-requisites of other courses
- Teacher's information