The course is offered to students of any study field.
Students will gain theoretical knewledge about modification of biomolecules based on conjugation reactions, introduction of labels and immobilization on surface of polymers and sensing surfaces. They will obtain overview of reagents for conjugation and labelling (enzymes, fluorophores, biotinylation, liposomes, nanoparticles).
Students will learn methods for conjugation of biomolecules with labels as enzymes, fluorophores and nanoparticles. They will manage principles of immobilization techniques for preparation of affinity supports and surfaces for purification and bioanalytical applications.
1. Modification of proteins and peptides, side chains of amino acids, prosthetic groups.
2. Modification of nucleotides, DNA, RNA. Modification of (poly)saccharides, glycoconjugates.
3. Insertion of additional reactive groups into biomolecules. Basic chemical reactions for conjugation.