C5440 Separation Methods
Faculty of ScienceAutumn 2024
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
In-person direct teaching - Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science - Timetable
- Wed 10:00–10:50 A08/309
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Main objectives can be summarized as follows: Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods; membrane separation processes and electromigration methods are mentioned as well.
- Learning outcomes
- After accomplishing the course students will be able:
- to understand and explain principles of the separation methods frequently used in organic chemistry in particular (e.g. GC, LC, HPLC, TLC and their modifications, crystallization, distillation, sublimation, extraction and electromigration methods);
- to choose appropriate separation method for solution of common analytical or preparative problems;
- to apply the knowledge obtained for setting basic parameters of the mentioned methods for development of simple procedures for sample separation. - Syllabus
- 1. Chromatography. Introduction, general theory and concepts. Main theoretical models. Retention equation, plate theory and kinetic theory. Elution ratio and resolution.
- 2. Gas chromatography. Van Deemter equation. GC techniques, block scheme of GC instruments. Carrier gas, sample injection, chomatographic columns, stationary phase. Enantioselective GC.
- 3. Main methods of GC detection. Flame Ionization Detector (FID), Thermal conductivity detector (TCD), Electron Capture Detector (ECD), GC-MS coupling.
- 4. Gas chromatography. Qualitative analysis, information from elution data, retention indexes (Kovats etc.). Selective detection. Quantitative analysis, calibration methods.
- 5. Liquid chromatography. Basic principles. Column chromatography, flash chromatography - main techniques, column preparation, detection, stationary phases. Planar chromatography - paper chromatography and TLC.
- 6. High performance liquid chromatography (HPLC). Block scheme of HPLC instrument. Mobile phase, pumps, degassing, gradient elution. Sample injection, HPLC columns, stationary phases. Enantioselective HPLC. HPLC detection - UV-Vis and fluorescence detectors, refractive index detector, evaporative light scattering detector. Chiroptical detection.
- 7. Ion exchange, gel permeation (size exclusion) and affinity chromatography. Ionexchangers and ion equilibria in IEC. Stationary phases and retention in SEC (GPC). Basic principles of affinity chromatography.
- 8. Sample preparation in chromatographic analysis. Derivatisation methods.
- 9. Extraction. Countercurrent extraction. Supercritical fluid extraction. MW assisted extraction.
- 10. Distillation. Basic principles. Rectification. Plate theory, distillation columns. Vacuum distillation, molecular distillation. Steam distillation, azeotropic and extractive distillation.
- 11. Crystallisation. Crystallisation from solution - nucleation and crystal growth. Crystallisation from melt. Resolution by means of crystallisation.
- 12. Sublimation. Sublimation techniques - gradient sublimation, lyophylization.
- 13. Electromigration separation methods. Basic principles, ion mobility, transport processes. Tiselius electrophoresis. Capillary electrophoresis. Isotachophoresis. SDS-PAGE.
- 14. Membrane permeation separation. Dialysis. Osmosis. Reverse osmosis and ultrafiltration.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Teaching methods
- Lectures
- Assessment methods
- Oral exam
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
C5440 Separation Methods
Faculty of ScienceAutumn 2023
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science - Timetable
- Tue 11:00–11:50 A08/309
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Main objectives can be summarized as follows: Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods; membrane separation processes and electromigration methods are mentioned as well.
- Learning outcomes
- After accomplishing the course students will be able:
- to understand and explain principles of the separation methods frequently used in organic chemistry in particular (e.g. GC, LC, HPLC, TLC and their modifications, crystallization, distillation, sublimation, extraction and electromigration methods);
- to choose appropriate separation method for solution of common analytical or preparative problems;
- to apply the knowledge obtained for setting basic parameters of the mentioned methods for development of simple procedures for sample separation. - Syllabus
- 1. Chromatography. Introduction, general theory and concepts. Main theoretical models. Retention equation, plate theory and kinetic theory. Elution ratio and resolution.
- 2. Gas chromatography. Van Deemter equation. GC techniques, block scheme of GC instruments. Carrier gas, sample injection, chomatographic columns, stationary phase. Enantioselective GC.
- 3. Main methods of GC detection. Flame Ionization Detector (FID), Thermal conductivity detector (TCD), Electron Capture Detector (ECD), GC-MS coupling.
- 4. Gas chromatography. Qualitative analysis, information from elution data, retention indexes (Kovats etc.). Selective detection. Quantitative analysis, calibration methods.
- 5. Liquid chromatography. Basic principles. Column chromatography, flash chromatography - main techniques, column preparation, detection, stationary phases. Planar chromatography - paper chromatography and TLC.
- 6. High performance liquid chromatography (HPLC). Block scheme of HPLC instrument. Mobile phase, pumps, degassing, gradient elution. Sample injection, HPLC columns, stationary phases. Enantioselective HPLC. HPLC detection - UV-Vis and fluorescence detectors, refractive index detector, evaporative light scattering detector. Chiroptical detection.
- 7. Ion exchange, gel permeation (size exclusion) and affinity chromatography. Ionexchangers and ion equilibria in IEC. Stationary phases and retention in SEC (GPC). Basic principles of affinity chromatography.
- 8. Sample preparation in chromatographic analysis. Derivatisation methods.
- 9. Extraction. Countercurrent extraction. Supercritical fluid extraction. MW assisted extraction.
- 10. Distillation. Basic principles. Rectification. Plate theory, distillation columns. Vacuum distillation, molecular distillation. Steam distillation, azeotropic and extractive distillation.
- 11. Crystallisation. Crystallisation from solution - nucleation and crystal growth. Crystallisation from melt. Resolution by means of crystallisation.
- 12. Sublimation. Sublimation techniques - gradient sublimation, lyophylization.
- 13. Electromigration separation methods. Basic principles, ion mobility, transport processes. Tiselius electrophoresis. Capillary electrophoresis. Isotachophoresis. SDS-PAGE.
- 14. Membrane permeation separation. Dialysis. Osmosis. Reverse osmosis and ultrafiltration.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Teaching methods
- Lectures
- Assessment methods
- Oral exam
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
C5440 Separation Methods
Faculty of ScienceAutumn 2022
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science - Timetable
- Tue 13:00–13:50 A08/309
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Main objectives can be summarized as follows: Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods; membrane separation processes and electromigration methods are mentioned as well.
- Learning outcomes
- After accomplishing the course students will be able:
- to understand and explain principles of the separation methods frequently used in organic chemistry in particular (e.g. GC, LC, HPLC, TLC and their modifications, crystallization, distillation, sublimation, extraction and electromigration methods);
- to choose appropriate separation method for solution of common analytical or preparative problems;
- to apply the knowledge obtained for setting basic parameters of the mentioned methods for development of simple procedures for sample separation. - Syllabus
- 1. Chromatography. Introduction, general theory and concepts. Main theoretical models. Retention equation, plate theory and kinetic theory. Elution ratio and resolution.
- 2. Gas chromatography. Van Deemter equation. GC techniques, block scheme of GC instruments. Carrier gas, sample injection, chomatographic columns, stationary phase. Enantioselective GC.
- 3. Main methods of GC detection. Flame Ionization Detector (FID), Thermal conductivity detector (TCD), Electron Capture Detector (ECD), GC-MS coupling.
- 4. Gas chromatography. Qualitative analysis, information from elution data, retention indexes (Kovats etc.). Selective detection. Quantitative analysis, calibration methods.
- 5. Liquid chromatography. Basic principles. Column chromatography, flash chromatography - main techniques, column preparation, detection, stationary phases. Planar chromatography - paper chromatography and TLC.
- 6. High performance liquid chromatography (HPLC). Block scheme of HPLC instrument. Mobile phase, pumps, degassing, gradient elution. Sample injection, HPLC columns, stationary phases. Enantioselective HPLC. HPLC detection - UV-Vis and fluorescence detectors, refractive index detector, evaporative light scattering detector. Chiroptical detection.
- 7. Ion exchange, gel permeation (size exclusion) and affinity chromatography. Ionexchangers and ion equilibria in IEC. Stationary phases and retention in SEC (GPC). Basic principles of affinity chromatography.
- 8. Sample preparation in chromatographic analysis. Derivatisation methods.
- 9. Extraction. Countercurrent extraction. Supercritical fluid extraction. MW assisted extraction.
- 10. Distillation. Basic principles. Rectification. Plate theory, distillation columns. Vacuum distillation, molecular distillation. Steam distillation, azeotropic and extractive distillation.
- 11. Crystallisation. Crystallisation from solution - nucleation and crystal growth. Crystallisation from melt. Resolution by means of crystallisation.
- 12. Sublimation. Sublimation techniques - gradient sublimation, lyophylization.
- 13. Electromigration separation methods. Basic principles, ion mobility, transport processes. Tiselius electrophoresis. Capillary electrophoresis. Isotachophoresis. SDS-PAGE.
- 14. Membrane permeation separation. Dialysis. Osmosis. Reverse osmosis and ultrafiltration.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Teaching methods
- Lectures
- Assessment methods
- Oral exam
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
C5440 Separation Methods
Faculty of Scienceautumn 2021
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science - Timetable
- Tue 14:00–14:50 A08/309
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Main objectives can be summarized as follows: Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods; membrane separation processes and electromigration methods are mentioned as well.
- Learning outcomes
- After accomplishing the course students will be able:
- to understand and explain principles of the separation methods frequently used in organic chemistry in particular (e.g. GC, LC, HPLC, TLC and their modifications, crystallization, distillation, sublimation, extraction and electromigration methods);
- to choose appropriate separation method for solution of common analytical or preparative problems;
- to apply the knowledge obtained for setting basic parameters of the mentioned methods for development of simple procedures for sample separation. - Syllabus
- 1. Chromatography. Introduction, general theory and concepts. Main theoretical models. Retention equation, plate theory and kinetic theory. Elution ratio and resolution.
- 2. Gas chromatography. Van Deemter equation. GC techniques, block scheme of GC instruments. Carrier gas, sample injection, chomatographic columns, stationary phase. Enantioselective GC.
- 3. Main methods of GC detection. Flame Ionization Detector (FID), Thermal conductivity detector (TCD), Electron Capture Detector (ECD), GC-MS coupling.
- 4. Gas chromatography. Qualitative analysis, information from elution data, retention indexes (Kovats etc.). Selective detection. Quantitative analysis, calibration methods.
- 5. Liquid chromatography. Basic principles. Column chromatography, flash chromatography - main techniques, column preparation, detection, stationary phases. Planar chromatography - paper chromatography and TLC.
- 6. High performance liquid chromatography (HPLC). Block scheme of HPLC instrument. Mobile phase, pumps, degassing, gradient elution. Sample injection, HPLC columns, stationary phases. Enantioselective HPLC. HPLC detection - UV-Vis and fluorescence detectors, refractive index detector, evaporative light scattering detector. Chiroptical detection.
- 7. Ion exchange, gel permeation (size exclusion) and affinity chromatography. Ionexchangers and ion equilibria in IEC. Stationary phases and retention in SEC (GPC). Basic principles of affinity chromatography.
- 8. Sample preparation in chromatographic analysis. Derivatisation methods.
- 9. Extraction. Countercurrent extraction. Supercritical fluid extraction. MW assisted extraction.
- 10. Distillation. Basic principles. Rectification. Plate theory, distillation columns. Vacuum distillation, molecular distillation. Steam distillation, azeotropic and extractive distillation.
- 11. Crystallisation. Crystallisation from solution - nucleation and crystal growth. Crystallisation from melt. Resolution by means of crystallisation.
- 12. Sublimation. Sublimation techniques - gradient sublimation, lyophylization.
- 13. Electromigration separation methods. Basic principles, ion mobility, transport processes. Tiselius electrophoresis. Capillary electrophoresis. Isotachophoresis. SDS-PAGE.
- 14. Membrane permeation separation. Dialysis. Osmosis. Reverse osmosis and ultrafiltration.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Teaching methods
- Lectures
- Assessment methods
- Oral exam
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
C5440 Separation Methods
Faculty of ScienceAutumn 2020
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science - Timetable
- Wed 14:00–14:50 prace doma
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Main objectives can be summarized as follows: Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods; membrane separation processes and electromigration methods are mentioned as well.
- Learning outcomes
- After accomplishing the course students will be able:
- to understand and explain principles of the separation methods frequently used in organic chemistry in particular (e.g. GC, LC, HPLC, TLC and their modifications, crystallization, distillation, sublimation, extraction and electromigration methods);
- to choose appropriate separation method for solution of common analytical or preparative problems;
- to apply the knowledge obtained for setting basic parameters of the mentioned methods for development of simple procedures for sample separation. - Syllabus
- 1. Chromatography. Introduction, general theory and concepts. Main theoretical models. Retention equation, plate theory and kinetic theory. Elution ratio and resolution.
- 2. Gas chromatography. Van Deemter equation. GC techniques, block scheme of GC instruments. Carrier gas, sample injection, chomatographic columns, stationary phase. Enantioselective GC.
- 3. Main methods of GC detection. Flame Ionization Detector (FID), Thermal conductivity detector (TCD), Electron Capture Detector (ECD), GC-MS coupling.
- 4. Gas chromatography. Qualitative analysis, information from elution data, retention indexes (Kovats etc.). Selective detection. Quantitative analysis, calibration methods.
- 5. Liquid chromatography. Basic principles. Column chromatography, flash chromatography - main techniques, column preparation, detection, stationary phases. Planar chromatography - paper chromatography and TLC.
- 6. High performance liquid chromatography (HPLC). Block scheme of HPLC instrument. Mobile phase, pumps, degassing, gradient elution. Sample injection, HPLC columns, stationary phases. Enantioselective HPLC. HPLC detection - UV-Vis and fluorescence detectors, refractive index detector, evaporative light scattering detector. Chiroptical detection.
- 7. Ion exchange, gel permeation (size exclusion) and affinity chromatography. Ionexchangers and ion equilibria in IEC. Stationary phases and retention in SEC (GPC). Basic principles of affinity chromatography.
- 8. Sample preparation in chromatographic analysis. Derivatisation methods.
- 9. Extraction. Countercurrent extraction. Supercritical fluid extraction. MW assisted extraction.
- 10. Distillation. Basic principles. Rectification. Plate theory, distillation columns. Vacuum distillation, molecular distillation. Steam distillation, azeotropic and extractive distillation.
- 11. Crystallisation. Crystallisation from solution - nucleation and crystal growth. Crystallisation from melt. Resolution by means of crystallisation.
- 12. Sublimation. Sublimation techniques - gradient sublimation, lyophylization.
- 13. Electromigration separation methods. Basic principles, ion mobility, transport processes. Tiselius electrophoresis. Capillary electrophoresis. Isotachophoresis. SDS-PAGE.
- 14. Membrane permeation separation. Dialysis. Osmosis. Reverse osmosis and ultrafiltration.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Teaching methods
- Lectures
- Assessment methods
- Oral exam
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
C5440 Separation Methods
Faculty of ScienceAutumn 2019
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science - Timetable
- Wed 9:00–9:50 A08/309
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Main objectives can be summarized as follows: Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods; membrane separation processes and electromigration methods are mentioned as well.
- Learning outcomes
- After accomplishing the course students will be able:
- to understand and explain principles of the separation methods frequently used in organic chemistry in particular (e.g. GC, LC, HPLC, TLC and their modifications, crystallization, distillation, sublimation, extraction and electromigration methods);
- to choose appropriate separation method for solution of common analytical or preparative problems;
- to apply the knowledge obtained for setting basic parameters of the mentioned methods for development of simple procedures for sample separation. - Syllabus
- 1. Chromatography. Introduction, general theory and concepts. Main theoretical models. Retention equation, plate theory and kinetic theory. Elution ratio and resolution.
- 2. Gas chromatography. Van Deemter equation. GC techniques, block scheme of GC instruments. Carrier gas, sample injection, chomatographic columns, stationary phase. Enantioselective GC.
- 3. Main methods of GC detection. Flame Ionization Detector (FID), Thermal conductivity detector (TCD), Electron Capture Detector (ECD), GC-MS coupling.
- 4. Gas chromatography. Qualitative analysis, information from elution data, retention indexes (Kovats etc.). Selective detection. Quantitative analysis, calibration methods.
- 5. Liquid chromatography. Basic principles. Column chromatography, flash chromatography - main techniques, column preparation, detection, stationary phases. Planar chromatography - paper chromatography and TLC.
- 6. High performance liquid chromatography (HPLC). Block scheme of HPLC instrument. Mobile phase, pumps, degassing, gradient elution. Sample injection, HPLC columns, stationary phases. Enantioselective HPLC. HPLC detection - UV-Vis and fluorescence detectors, refractive index detector, evaporative light scattering detector. Chiroptical detection.
- 7. Ion exchange, gel permeation (size exclusion) and affinity chromatography. Ionexchangers and ion equilibria in IEC. Stationary phases and retention in SEC (GPC). Basic principles of affinity chromatography.
- 8. Sample preparation in chromatographic analysis. Derivatisation methods.
- 9. Extraction. Countercurrent extraction. Supercritical fluid extraction. MW assisted extraction.
- 10. Distillation. Basic principles. Rectification. Plate theory, distillation columns. Vacuum distillation, molecular distillation. Steam distillation, azeotropic and extractive distillation.
- 11. Crystallisation. Crystallisation from solution - nucleation and crystal growth. Crystallisation from melt. Resolution by means of crystallisation.
- 12. Sublimation. Sublimation techniques - gradient sublimation, lyophylization.
- 13. Electromigration separation methods. Basic principles, ion mobility, transport processes. Tiselius electrophoresis. Capillary electrophoresis. Isotachophoresis. SDS-PAGE.
- 14. Membrane permeation separation. Dialysis. Osmosis. Reverse osmosis and ultrafiltration.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Teaching methods
- Lectures
- Assessment methods
- Oral exam
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
C5440 Separation Methods
Faculty of ScienceAutumn 2018
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science - Timetable
- Mon 17. 9. to Fri 14. 12. Tue 16:00–16:50 A08/309
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Main objectives can be summarized as follows: Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods; membrane separation processes and electromigration methods are mentioned as well.
- Syllabus
- 1. Chromatography. Introduction, general theory and concepts. Main theoretical models. Retention equation, plate theory and kinetic theory. Elution ratio and resolution.
- 2. Gas chromatography. Van Deemter equation. GC techniques, block scheme of GC instruments. Carrier gas, sample injection, chomatographic columns, stationary phase. Enantioselective GC.
- 3. Main methods of GC detection. Flame Ionization Detector (FID), Thermal conductivity detector (TCD), Electron Capture Detector (ECD), GC-MS coupling.
- 4. Gas chromatography. Qualitative analysis, information from elution data, retention indexes (Kovats etc.). Selective detection. Quantitative analysis, calibration methods.
- 5. Liquid chromatography. Basic principles. Column chromatography, flash chromatography - main techniques, column preparation, detection, stationary phases. Planar chromatography - paper chromatography and TLC.
- 6. High performance liquid chromatography (HPLC). Block scheme of HPLC instrument. Mobile phase, pumps, degassing, gradient elution. Sample injection, HPLC columns, stationary phases. Enantioselective HPLC. HPLC detection - UV-Vis and fluorescence detectors, refractive index detector, evaporative light scattering detector. Chiroptical detection.
- 7. Ion exchange, gel permeation (size exclusion) and affinity chromatography. Ionexchangers and ion equilibria in IEC. Stationary phases and retention in SEC (GPC). Basic principles of affinity chromatography.
- 8. Sample preparation in chromatographic analysis. Derivatisation methods.
- 9. Extraction. Countercurrent extraction. Supercritical fluid extraction. MW assisted extraction.
- 10. Distillation. Basic principles. Rectification. Plate theory, distillation columns. Vacuum distillation, molecular distillation. Steam distillation, azeotropic and extractive distillation.
- 11. Crystallisation. Crystallisation from solution - nucleation and crystal growth. Crystallisation from melt. Resolution by means of crystallisation.
- 12. Sublimation. Sublimation techniques - gradient sublimation, lyophylization.
- 13. Electromigration separation methods. Basic principles, ion mobility, transport processes. Tiselius electrophoresis. Capillary electrophoresis. Isotachophoresis. SDS-PAGE.
- 14. Membrane permeation separation. Dialysis. Osmosis. Reverse osmosis and ultrafiltration.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Teaching methods
- Lectures
- Assessment methods
- Oral exam
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
C5440 Separation Methods
Faculty of Scienceautumn 2017
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science - Timetable
- Mon 18. 9. to Fri 15. 12. Tue 16:00–16:50 A08/309
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Main objectives can be summarized as follows: Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods; membrane separation processes and electromigration methods are mentioned as well.
- Syllabus
- 1. Chromatography. Introduction, general theory and concepts. Main theoretical models. Retention equation, plate theory and kinetic theory. Elution ratio and resolution.
- 2. Gas chromatography. Van Deemter equation. GC techniques, block scheme of GC instruments. Carrier gas, sample injection, chomatographic columns, stationary phase. Enantioselective GC.
- 3. Main methods of GC detection. Flame Ionization Detector (FID), Thermal conductivity detector (TCD), Electron Capture Detector (ECD), GC-MS coupling.
- 4. Gas chromatography. Qualitative analysis, information from elution data, retention indexes (Kovats etc.). Selective detection. Quantitative analysis, calibration methods.
- 5. Liquid chromatography. Basic principles. Column chromatography, flash chromatography - main techniques, column preparation, detection, stationary phases. Planar chromatography - paper chromatography and TLC.
- 6. High performance liquid chromatography (HPLC). Block scheme of HPLC instrument. Mobile phase, pumps, degassing, gradient elution. Sample injection, HPLC columns, stationary phases. Enantioselective HPLC. HPLC detection - UV-Vis and fluorescence detectors, refractive index detector, evaporative light scattering detector. Chiroptical detection.
- 7. Ion exchange, gel permeation (size exclusion) and affinity chromatography. Ionexchangers and ion equilibria in IEC. Stationary phases and retention in SEC (GPC). Basic principles of affinity chromatography.
- 8. Sample preparation in chromatographic analysis. Derivatisation methods.
- 9. Extraction. Countercurrent extraction. Supercritical fluid extraction. MW assisted extraction.
- 10. Distillation. Basic principles. Rectification. Plate theory, distillation columns. Vacuum distillation, molecular distillation. Steam distillation, azeotropic and extractive distillation.
- 11. Crystallisation. Crystallisation from solution - nucleation and crystal growth. Crystallisation from melt. Resolution by means of crystallisation.
- 12. Sublimation. Sublimation techniques - gradient sublimation, lyophylization.
- 13. Electromigration separation methods. Basic principles, ion mobility, transport processes. Tiselius electrophoresis. Capillary electrophoresis. Isotachophoresis. SDS-PAGE.
- 14. Membrane permeation separation. Dialysis. Osmosis. Reverse osmosis and ultrafiltration.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Teaching methods
- Lectures
- Assessment methods
- Oral exam
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
C5440 Separation Methods
Faculty of ScienceAutumn 2016
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science - Timetable
- Mon 19. 9. to Sun 18. 12. Tue 16:00–16:50 A08/309
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Main objectives can be summarized as follows: Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods; membrane separation processes and electromigration methods are mentioned as well.
- Syllabus
- 1. Chromatography. Introduction, general theory and concepts. Main theoretical models. Retention equation, plate theory and kinetic theory. Elution ratio and resolution.
- 2. Gas chromatography. Van Deemter equation. GC techniques, block scheme of GC instruments. Carrier gas, sample injection, chomatographic columns, stationary phase. Enantioselective GC.
- 3. Main methods of GC detection. Flame Ionization Detector (FID), Thermal conductivity detector (TCD), Electron Capture Detector (ECD), GC-MS coupling.
- 4. Gas chromatography. Qualitative analysis, information from elution data, retention indexes (Kovats etc.). Selective detection. Quantitative analysis, calibration methods.
- 5. Liquid chromatography. Basic principles. Column chromatography, flash chromatography - main techniques, column preparation, detection, stationary phases. Planar chromatography - paper chromatography and TLC.
- 6. High performance liquid chromatography (HPLC). Block scheme of HPLC instrument. Mobile phase, pumps, degassing, gradient elution. Sample injection, HPLC columns, stationary phases. Enantioselective HPLC. HPLC detection - UV-Vis and fluorescence detectors, refractive index detector, evaporative light scattering detector. Chiroptical detection.
- 7. Ion exchange, gel permeation (size exclusion) and affinity chromatography. Ionexchangers and ion equilibria in IEC. Stationary phases and retention in SEC (GPC). Basic principles of affinity chromatography.
- 8. Sample preparation in chromatographic analysis. Derivatisation methods.
- 9. Extraction. Countercurrent extraction. Supercritical fluid extraction. MW assisted extraction.
- 10. Distillation. Basic principles. Rectification. Plate theory, distillation columns. Vacuum distillation, molecular distillation. Steam distillation, azeotropic and extractive distillation.
- 11. Crystallisation. Crystallisation from solution - nucleation and crystal growth. Crystallisation from melt. Resolution by means of crystallisation.
- 12. Sublimation. Sublimation techniques - gradient sublimation, lyophylization.
- 13. Electromigration separation methods. Basic principles, ion mobility, transport processes. Tiselius electrophoresis. Capillary electrophoresis. Isotachophoresis. SDS-PAGE.
- 14. Membrane permeation separation. Dialysis. Osmosis. Reverse osmosis and ultrafiltration.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Teaching methods
- Lectures
- Assessment methods
- Oral exam
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
C5440 Separation Methods
Faculty of ScienceAutumn 2015
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science - Timetable
- Tue 12:00–12:50 B11/235
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Main objectives can be summarized as follows: Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods; membrane separation processes and electromigration methods are mentioned as well.
- Syllabus
- 1. Chromatography. Introduction, general theory and concepts. Main theoretical models. Retention equation, plate theory and kinetic theory. Elution ratio and resolution.
- 2. Gas chromatography. Van Deemter equation. GC techniques, block scheme of GC instruments. Carrier gas, sample injection, chomatographic columns, stationary phase. Enantioselective GC.
- 3. Main methods of GC detection. Flame Ionization Detector (FID), Thermal conductivity detector (TCD), Electron Capture Detector (ECD), GC-MS coupling.
- 4. Gas chromatography. Qualitative analysis, information from elution data, retention indexes (Kovats etc.). Selective detection. Quantitative analysis, calibration methods.
- 5. Liquid chromatography. Basic principles. Column chromatography, flash chromatography - main techniques, column preparation, detection, stationary phases. Planar chromatography - paper chromatography and TLC.
- 6. High performance liquid chromatography (HPLC). Block scheme of HPLC instrument. Mobile phase, pumps, degassing, gradient elution. Sample injection, HPLC columns, stationary phases. Enantioselective HPLC. HPLC detection - UV-Vis and fluorescence detectors, refractive index detector, evaporative light scattering detector. Chiroptical detection.
- 7. Ion exchange, gel permeation (size exclusion) and affinity chromatography. Ionexchangers and ion equilibria in IEC. Stationary phases and retention in SEC (GPC). Basic principles of affinity chromatography.
- 8. Sample preparation in chromatographic analysis. Derivatisation methods.
- 9. Extraction. Countercurrent extraction. Supercritical fluid extraction. MW assisted extraction.
- 10. Distillation. Basic principles. Rectification. Plate theory, distillation columns. Vacuum distillation, molecular distillation. Steam distillation, azeotropic and extractive distillation.
- 11. Crystallisation. Crystallisation from solution - nucleation and crystal growth. Crystallisation from melt. Resolution by means of crystallisation.
- 12. Sublimation. Sublimation techniques - gradient sublimation, lyophylization.
- 13. Electromigration separation methods. Basic principles, ion mobility, transport processes. Tiselius electrophoresis. Capillary electrophoresis. Isotachophoresis. SDS-PAGE.
- 14. Membrane permeation separation. Dialysis. Osmosis. Reverse osmosis and ultrafiltration.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Teaching methods
- Lectures
- Assessment methods
- Oral exam
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
C5440 Separation Methods
Faculty of ScienceAutumn 2014
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science - Timetable
- Tue 11:00–11:50 A08/309
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Main objectives can be summarized as follows: Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods; membrane separation processes and electromigration methods are mentioned as well.
- Syllabus
- 1. Chromatography. Introduction, general theory and concepts. Main theoretical models. Retention equation, plate theory and kinetic theory. Elution ratio and resolution.
- 2. Gas chromatography. Van Deemter equation. GC techniques, block scheme of GC instruments. Carrier gas, sample injection, chomatographic columns, stationary phase. Enantioselective GC.
- 3. Main methods of GC detection. Flame Ionization Detector (FID), Thermal conductivity detector (TCD), Electron Capture Detector (ECD), GC-MS coupling.
- 4. Gas chromatography. Qualitative analysis, information from elution data, retention indexes (Kovats etc.). Selective detection. Quantitative analysis, calibration methods.
- 5. Liquid chromatography. Basic principles. Column chromatography, flash chromatography - main techniques, column preparation, detection, stationary phases. Planar chromatography - paper chromatography and TLC.
- 6. High performance liquid chromatography (HPLC). Block scheme of HPLC instrument. Mobile phase, pumps, degassing, gradient elution. Sample injection, HPLC columns, stationary phases. Enantioselective HPLC. HPLC detection - UV-Vis and fluorescence detectors, refractive index detector, evaporative light scattering detector. Chiroptical detection.
- 7. Ion exchange, gel permeation (size exclusion) and affinity chromatography. Ionexchangers and ion equilibria in IEC. Stationary phases and retention in SEC (GPC). Basic principles of affinity chromatography.
- 8. Sample preparation in chromatographic analysis. Derivatisation methods.
- 9. Extraction. Countercurrent extraction. Supercritical fluid extraction. MW assisted extraction.
- 10. Distillation. Basic principles. Rectification. Plate theory, distillation columns. Vacuum distillation, molecular distillation. Steam distillation, azeotropic and extractive distillation.
- 11. Crystallisation. Crystallisation from solution - nucleation and crystal growth. Crystallisation from melt. Resolution by means of crystallisation.
- 12. Sublimation. Sublimation techniques - gradient sublimation, lyophylization.
- 13. Electromigration separation methods. Basic principles, ion mobility, transport processes. Tiselius electrophoresis. Capillary electrophoresis. Isotachophoresis. SDS-PAGE.
- 14. Membrane permeation separation. Dialysis. Osmosis. Reverse osmosis and ultrafiltration.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Teaching methods
- Lectures
- Assessment methods
- Oral exam
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
C5440 Separation Methods
Faculty of ScienceAutumn 2013
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science - Timetable
- Mon 11:00–11:50 B11/235
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Main objectives can be summarized as follows: Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods; membrane separation processes and electromigration methods are mentioned as well.
- Syllabus
- 1. Chromatography. Introduction, general theory and concepts. Main theoretical models. Retention equation, plate theory and kinetic theory. Elution ratio and resolution.
- 2. Gas chromatography. Van Deemter equation. GC techniques, block scheme of GC instruments. Carrier gas, sample injection, chomatographic columns, stationary phase. Enantioselective GC.
- 3. Main methods of GC detection. Flame Ionization Detector (FID), Thermal conductivity detector (TCD), Electron Capture Detector (ECD), GC-MS coupling.
- 4. Gas chromatography. Qualitative analysis, information from elution data, retention indexes (Kovats etc.). Selective detection. Quantitative analysis, calibration methods.
- 5. Liquid chromatography. Basic principles. Column chromatography, flash chromatography - main techniques, column preparation, detection, stationary phases. Planar chromatography - paper chromatography and TLC.
- 6. High performance liquid chromatography (HPLC). Block scheme of HPLC instrument. Mobile phase, pumps, degassing, gradient elution. Sample injection, HPLC columns, stationary phases. Enantioselective HPLC. HPLC detection - UV-Vis and fluorescence detectors, refractive index detector, evaporative light scattering detector. Chiroptical detection.
- 7. Ion exchange, gel permeation (size exclusion) and affinity chromatography. Ionexchangers and ion equilibria in IEC. Stationary phases and retention in SEC (GPC). Basic principles of affinity chromatography.
- 8. Sample preparation in chromatographic analysis. Derivatisation methods.
- 9. Extraction. Countercurrent extraction. Supercritical fluid extraction. MW assisted extraction.
- 10. Distillation. Basic principles. Rectification. Plate theory, distillation columns. Vacuum distillation, molecular distillation. Steam distillation, azeotropic and extractive distillation.
- 11. Crystallisation. Crystallisation from solution - nucleation and crystal growth. Crystallisation from melt. Resolution by means of crystallisation.
- 12. Sublimation. Sublimation techniques - gradient sublimation, lyophylization.
- 13. Electromigration separation methods. Basic principles, ion mobility, transport processes. Tiselius electrophoresis. Capillary electrophoresis. Isotachophoresis. SDS-PAGE.
- 14. Membrane permeation separation. Dialysis. Osmosis. Reverse osmosis and ultrafiltration.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Teaching methods
- Lectures
- Assessment methods
- Oral exam
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
C5440 Separation Methods
Faculty of ScienceAutumn 2012
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science - Timetable
- Wed 11:00–11:50 B11/205
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Main objectives can be summarized as follows: Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods; membrane separation processes and electromigration methods are mentioned as well.
- Syllabus
- 1. Chromatography. Introduction, general theory and concepts. Main theoretical models. Retention equation, plate theory and kinetic theory. Elution ratio and resolution.
- 2. Gas chromatography. Van Deemter equation. GC techniques, block scheme of GC instruments. Carrier gas, sample injection, chomatographic columns, stationary phase. Enantioselective GC.
- 3. Main methods of GC detection. Flame Ionization Detector (FID), Thermal conductivity detector (TCD), Electron Capture Detector (ECD), GC-MS coupling.
- 4. Gas chromatography. Qualitative analysis, information from elution data, retention indexes (Kovats etc.). Selective detection. Quantitative analysis, calibration methods.
- 5. Liquid chromatography. Basic principles. Column chromatography, flash chromatography - main techniques, column preparation, detection, stationary phases. Planar chromatography - paper chromatography and TLC.
- 6. High performance liquid chromatography (HPLC). Block scheme of HPLC instrument. Mobile phase, pumps, degassing, gradient elution. Sample injection, HPLC columns, stationary phases. Enantioselective HPLC. HPLC detection - UV-Vis and fluorescence detectors, refractive index detector, evaporative light scattering detector. Chiroptical detection.
- 7. Ion exchange, gel permeation (size exclusion) and affinity chromatography. Ionexchangers and ion equilibria in IEC. Stationary phases and retention in SEC (GPC). Basic principles of affinity chromatography.
- 8. Sample preparation in chromatographic analysis. Derivatisation methods.
- 9. Extraction. Countercurrent extraction. Supercritical fluid extraction. MW assisted extraction.
- 10. Distillation. Basic principles. Rectification. Plate theory, distillation columns. Vacuum distillation, molecular distillation. Steam distillation, azeotropic and extractive distillation.
- 11. Crystallisation. Crystallisation from solution - nucleation and crystal growth. Crystallisation from melt. Resolution by means of crystallisation.
- 12. Sublimation. Sublimation techniques - gradient sublimation, lyophylization.
- 13. Electromigration separation methods. Basic principles, ion mobility, transport processes. Tiselius electrophoresis. Capillary electrophoresis. Isotachophoresis. SDS-PAGE.
- 14. Membrane permeation separation. Dialysis. Osmosis. Reverse osmosis and ultrafiltration.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Teaching methods
- Lectures
- Assessment methods
- Oral exam
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
C5440 Separation Methods
Faculty of ScienceAutumn 2011
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science - Timetable
- Wed 13:00–13:50 B11/205
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Main objectives can be summarized as follows: Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods; membrane separation processes and electromigration methods are mentioned as well.
- Syllabus
- 1. Chromatography. Introduction, general theory and concepts. Main theoretical models. Retention equation, plate theory and kinetic theory. Elution ratio and resolution.
- 2. Gas chromatography. Van Deemter equation. GC techniques, block scheme of GC instruments. Carrier gas, sample injection, chomatographic columns, stationary phase. Enantioselective GC.
- 3. Main methods of GC detection. Flame Ionization Detector (FID), Thermal conductivity detector (TCD), Electron Capture Detector (ECD), GC-MS coupling.
- 4. Gas chromatography. Qualitative analysis, information from elution data, retention indexes (Kovats etc.). Selective detection. Quantitative analysis, calibration methods.
- 5. Liquid chromatography. Basic principles. Column chromatography, flash chromatography - main techniques, column preparation, detection, stationary phases. Planar chromatography - paper chromatography and TLC.
- 6. High performance liquid chromatography (HPLC). Block scheme of HPLC instrument. Mobile phase, pumps, degassing, gradient elution. Sample injection, HPLC columns, stationary phases. Enantioselective HPLC. HPLC detection - UV-Vis and fluorescence detectors, refractive index detector, evaporative light scattering detector. Chiroptical detection.
- 7. Ion exchange, gel permeation (size exclusion) and affinity chromatography. Ionexchangers and ion equilibria in IEC. Stationary phases and retention in SEC (GPC). Basic principles of affinity chromatography.
- 8. Sample preparation in chromatographic analysis. Derivatisation methods.
- 9. Extraction. Countercurrent extraction. Supercritical fluid extraction. MW assisted extraction.
- 10. Distillation. Basic principles. Rectification. Plate theory, distillation columns. Vacuum distillation, molecular distillation. Steam distillation, azeotropic and extractive distillation.
- 11. Crystallisation. Crystallisation from solution - nucleation and crystal growth. Crystallisation from melt. Resolution by means of crystallisation.
- 12. Sublimation. Sublimation techniques - gradient sublimation, lyophylization.
- 13. Electromigration separation methods. Basic principles, ion mobility, transport processes. Tiselius electrophoresis. Capillary electrophoresis. Isotachophoresis. SDS-PAGE.
- 14. Membrane permeation separation. Dialysis. Osmosis. Reverse osmosis and ultrafiltration.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Teaching methods
- Lectures
- Assessment methods
- Oral exam
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
C5440 Separation Methods
Faculty of ScienceAutumn 2010
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science - Timetable
- Mon 10:00–10:50 B11/235
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Main objectives can be summarized as follows: Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods; membrane separation processes and electromigration methods are mentioned as well.
- Syllabus
- 1. Chromatography. Introduction, general theory and concepts. Main theoretical models. Retention equation, plate theory and kinetic theory. Elution ratio and resolution.
- 2. Gas chromatography. Van Deemter equation. GC techniques, block scheme of GC instruments. Carrier gas, sample injection, chomatographic columns, stationary phase. Enantioselective GC.
- 3. Main methods of GC detection. Flame Ionization Detector (FID), Thermal conductivity detector (TCD), Electron Capture Detector (ECD), GC-MS coupling.
- 4. Gas chromatography. Qualitative analysis, information from elution data, retention indexes (Kovats etc.). Selective detection. Quantitative analysis, calibration methods.
- 5. Liquid chromatography. Basic principles. Column chromatography, flash chromatography - main techniques, column preparation, detection, stationary phases. Planar chromatography - paper chromatography and TLC.
- 6. High performance liquid chromatography (HPLC). Block scheme of HPLC instrument. Mobile phase, pumps, degassing, gradient elution. Sample injection, HPLC columns, stationary phases. Enantioselective HPLC. HPLC detection - UV-Vis and fluorescence detectors, refractive index detector, evaporative light scattering detector. Chiroptical detection.
- 7. Ion exchange, gel permeation (size exclusion) and affinity chromatography. Ionexchangers and ion equilibria in IEC. Stationary phases and retention in SEC (GPC). Basic principles of affinity chromatography.
- 8. Sample preparation in chromatographic analysis. Derivatisation methods.
- 9. Extraction. Countercurrent extraction. Supercritical fluid extraction. MW assisted extraction.
- 10. Distillation. Basic principles. Rectification. Plate theory, distillation columns. Vacuum distillation, molecular distillation. Steam distillation, azeotropic and extractive distillation.
- 11. Crystallisation. Crystallisation from solution - nucleation and crystal growth. Crystallisation from melt. Resolution by means of crystallisation.
- 12. Sublimation. Sublimation techniques - gradient sublimation, lyophylization.
- 13. Electromigration separation methods. Basic principles, ion mobility, transport processes. Tiselius electrophoresis. Capillary electrophoresis. Isotachophoresis. SDS-PAGE.
- 14. Membrane permeation separation. Dialysis. Osmosis. Reverse osmosis and ultrafiltration.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Teaching methods
- Lectures
- Assessment methods
- Oral exam
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
C5440 Separation Methods
Faculty of ScienceAutumn 2009
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science - Timetable
- Mon 11:00–11:50 B09/316
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Main objectives can be summarized as follows: Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods; membrane separation processes and electromigration methods are mentioned as well.
- Syllabus
- 1. Chromatography. Introduction, general theory and concepts. Main theoretical models. Retention equation, plate theory and kinetic theory. Elution ratio and resolution.
- 2. Gas chromatography. Van Deemter equation. GC techniques, block scheme of GC instruments. Carrier gas, sample injection, chomatographic columns, stationary phase. Enantioselective GC.
- 3. Main methods of GC detection. Flame Ionization Detector (FID), Thermal conductivity detector (TCD), Electron Capture Detector (ECD), GC-MS coupling.
- 4. Gas chromatography. Qualitative analysis, information from elution data, retention indexes (Kovats etc.). Selective detection. Quantitative analysis, calibration methods.
- 5. Liquid chromatography. Basic principles. Column chromatography, flash chromatography - main techniques, column preparation, detection, stationary phases. Planar chromatography - paper chromatography and TLC.
- 6. High performance liquid chromatography (HPLC). Block scheme of HPLC instrument. Mobile phase, pumps, degassing, gradient elution. Sample injection, HPLC columns, stationary phases. Enantioselective HPLC. HPLC detection - UV-Vis and fluorescence detectors, refractive index detector, evaporative light scattering detector. Chiroptical detection.
- 7. Ion exchange, gel permeation (size exclusion) and affinity chromatography. Ionexchangers and ion equilibria in IEC. Stationary phases and retention in SEC (GPC). Basic principles of affinity chromatography.
- 8. Sample preparation in chromatographic analysis. Derivatisation methods.
- 9. Extraction. Countercurrent extraction. Supercritical fluid extraction. MW assisted extraction.
- 10. Distillation. Basic principles. Rectification. Plate theory, distillation columns. Vacuum distillation, molecular distillation. Steam distillation, azeotropic and extractive distillation.
- 11. Crystallisation. Crystallisation from solution - nucleation and crystal growth. Crystallisation from melt. Resolution by means of crystallisation.
- 12. Sublimation. Sublimation techniques - gradient sublimation, lyophylization.
- 13. Electromigration separation methods. Basic principles, ion mobility, transport processes. Tiselius electrophoresis. Capillary electrophoresis. Isotachophoresis. SDS-PAGE.
- 14. Membrane permeation separation. Dialysis. Osmosis. Reverse osmosis and ultrafiltration.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Teaching methods
- Lectures
- Assessment methods
- Oral exam
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
C5440 Separation Methods
Faculty of ScienceAutumn 2008
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science - Timetable
- Wed 15:00–15:50 B09/316
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Main objectives can be summarized as follows: Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods; membrane separation processes and electromigration methods are mentioned as well.
- Syllabus
- 1. Chromatography. Introduction, general theory and concepts. Main theoretical models. Retention equation, plate theory and kinetic theory. Elution ratio and resolution.
- 2. Gas chromatography. Van Deemter equation. GC techniques, block scheme of GC instruments. Carrier gas, sample injection, chomatographic columns, stationary phase. Enantioselective GC.
- 3. Main methods of GC detection. Flame Ionization Detector (FID), Thermal conductivity detector (TCD), Electron Capture Detector (ECD), GC-MS coupling.
- 4. Gas chromatography. Qualitative analysis, information from elution data, retention indexes (Kovats etc.). Selective detection. Quantitative analysis, calibration methods.
- 5. Liquid chromatography. Basic principles. Column chromatography, flash chromatography - main techniques, column preparation, detection, stationary phases. Planar chromatography - paper chromatography and TLC.
- 6. High performance liquid chromatography (HPLC). Block scheme of HPLC instrument. Mobile phase, pumps, degassing, gradient elution. Sample injection, HPLC columns, stationary phases. Enantioselective HPLC. HPLC detection - UV-Vis and fluorescence detectors, refractive index detector, evaporative light scattering detector. Chiroptical detection.
- 7. Ion exchange, gel permeation (size exclusion) and affinity chromatography. Ionexchangers and ion equilibria in IEC. Stationary phases and retention in SEC (GPC). Basic principles of affinity chromatography.
- 8. Sample preparation in chromatographic analysis. Derivatisation methods.
- 9. Extraction. Countercurrent extraction. Supercritical fluid extraction. MW assisted extraction.
- 10. Distillation. Basic principles. Rectification. Plate theory, distillation columns. Vacuum distillation, molecular distillation. Steam distillation, azeotropic and extractive distillation.
- 11. Crystallisation. Crystallisation from solution - nucleation and crystal growth. Crystallisation from melt. Resolution by means of crystallisation.
- 12. Sublimation. Sublimation techniques - gradient sublimation, lyophylization.
- 13. Electromigration separation methods. Basic principles, ion mobility, transport processes. Tiselius electrophoresis. Capillary electrophoresis. Isotachophoresis. SDS-PAGE.
- 14. Membrane permeation separation. Dialysis. Osmosis. Reverse osmosis and ultrafiltration.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Assessment methods
- lecture with oral exam
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
C5440 Separation Methods
Faculty of ScienceAutumn 2007
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science - Timetable
- Wed 14:00–14:50 A08/309
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods are the main topics of the course; membrane separation processes and electromigration methods are mentioned as well.
- Syllabus (in Czech)
- 1. Chromatografické metody. Úvod, základní teorie a pojmy. Klasifikace chromatografických systémů a postupů. Základní teoretické modely popisující chromatografii. Retenční rovnice, teoretické patro, faktory ovlivňující separační účinnost, eluční poměr a rozlisení.
- 2. Plynová chromatografie. Základní pojmy. Van Deemterova rovnice.Technika GC. Blokové schema plynového chromatografu. Nosný plyn, techniky dávkování vzorku, náplňové a kapilární chromatografické kolony, stacionární fáze, enantioselektivní kolony.
- 3. Plynová chromatografie. Hlavní metody detekce pouzívané v GC. Plamenově ionizační detektor (FID), tepelně vodivostní detekce (TCD), elektronový záchyt (ECD), spojení GC s hmotnostní spektrometrem (GC-MS).
- 4. Plynová chromatografie. Kvalitativní analýza, identifikace z elučních údajů, eluční závislosti (Kovatsovy indexy), selektivní detektory. Kvantitavní analýza, faktory ovlivňující přesnost kvantitativní analýzy, metody kalibrace.
- 5. Kapalinová chromatografie. Základní pojmy. Chromatografie na sloupci, flash chromatografie - základy techniky, příprava kolon, detekce, stacionární fáze. Planární chromatografie. Papírová chromatografie, tenkovrstvá chromatografie.
- 6. Vysokoúčinná kapalinová chromatografie (HPLC). Blokové schema HPLC. Mobilní fáze, čerpadla, odplynění, gradient mobilní fáze. Dávkování vzorku. Kolony pro HPLC, stacionární fáze, výběr kolony, enantioselektivní fáze. Detektory pro HPLC, UV-VIS a fluorescenční detektor, refraktometr, ELSD, polarimetrická detekce.
- ) 7. Iontová, gelová a afinitní chromatografie. Klasifikace ionexů a jejich vlastnosti - selektivita ionexů. Rovnováhy při výměně iontů. Chelatační sorbenty. Vylučovací kapalinová chromatografie (GPC, SEC), retence v SEC, stacionární fáze - anorganické a organické gely. Biospecifická afinitní chromatografie - retence, bioafinitní sorbenty a podmínky chromatografie.
- 8. Příprava vzorku pro chromatografickou analýzu. Problém získání reprezentativního vzorku. Isolační a koncentrační techniky. Derivatizační metody v GC a HPLC.
- 9. Extrakční metody. Rovnováhy mezi dvěma kapalnými fázemi. Extrakce a roztřepávání. Superkritická extrakce. Voolba a vlastnosti superkritických mobilních fází. Příklady aplikací.
- 10. Destilace. Obecné principy. Prostá desilace. Rektifikace, pojem teoretického patra, faktory ovlivňující separační účinnost kolon, typy destilačních kolon. Destilace za snízeného tlaku, molekulová destilace, destilace s vodní parou, azeotropní destilace, extrakční destilace.
- 11. Krystalizace. Vymezení pojmu. Krystalizace z roztoku, vznik krystalizačních center a růst krystalu. Krystalizace z taveniny, zónové tavení. Krystalizační metody dělení enantiomerů, racemická sloučenina, racemát. Přímá krystalizace a dělení přes diastereomerní sloučeniny.
- 12. Membránové separační procesy. Membránové procesy v gradientu chemického potenciálu, dialýza, osmóza. Procesy v gradientu elektrického potenciálu, elektrodialýza, elektroosmóza. Procesy v gradientu tlaku, reversní osmóza, ultrafiltrace.
- 13. Elektroforetické metody. Základní pojmy, pohyblivost iontů, transportní děje. Doprovodné děje při elektroforetické separaci. Volná elektroforéza, Kapilární zónová elektroforéza, izotachoforéza.
- 14. Další separační metody. Sublimace. Vymezení pojmu. Různé techniky sublimace, gradientová sublimace, mrazová sublimace - lyofilizace. Sedimentační metody. Základní pojmy, sedimentační analýza.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Assessment methods (in Czech)
- Přednáška zakončená ústní zkouškou, popřípadě kolokviem.
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
C5440 Separation Methods
Faculty of ScienceAutumn 2006
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science - Timetable
- Thu 9:00–9:50 03021
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods are the main topics of the course; membrane separation processes and electromigration methods are mentioned as well.
- Syllabus (in Czech)
- 1. Chromatografické metody. Úvod, základní teorie a pojmy. Klasifikace chromatografických systémů a postupů. Základní teoretické modely popisující chromatografii. Retenční rovnice, teoretické patro, faktory ovlivňující separační účinnost, eluční poměr a rozlisení.
- 2. Plynová chromatografie. Základní pojmy. Van Deemterova rovnice.Technika GC. Blokové schema plynového chromatografu. Nosný plyn, techniky dávkování vzorku, náplňové a kapilární chromatografické kolony, stacionární fáze, enantioselektivní kolony.
- 3. Plynová chromatografie. Hlavní metody detekce pouzívané v GC. Plamenově ionizační detektor (FID), tepelně vodivostní detekce (TCD), elektronový záchyt (ECD), spojení GC s hmotnostní spektrometrem (GC-MS).
- 4. Plynová chromatografie. Kvalitativní analýza, identifikace z elučních údajů, eluční závislosti (Kovatsovy indexy), selektivní detektory. Kvantitavní analýza, faktory ovlivňující přesnost kvantitativní analýzy, metody kalibrace.
- 5. Kapalinová chromatografie. Základní pojmy. Chromatografie na sloupci, flash chromatografie - základy techniky, příprava kolon, detekce, stacionární fáze. Planární chromatografie. Papírová chromatografie, tenkovrstvá chromatografie.
- 6. Vysokoúčinná kapalinová chromatografie (HPLC). Blokové schema HPLC. Mobilní fáze, čerpadla, odplynění, gradient mobilní fáze. Dávkování vzorku. Kolony pro HPLC, stacionární fáze, výběr kolony, enantioselektivní fáze. Detektory pro HPLC, UV-VIS a fluorescenční detektor, refraktometr, ELSD, polarimetrická detekce.
- ) 7. Iontová, gelová a afinitní chromatografie. Klasifikace ionexů a jejich vlastnosti - selektivita ionexů. Rovnováhy při výměně iontů. Chelatační sorbenty. Vylučovací kapalinová chromatografie (GPC, SEC), retence v SEC, stacionární fáze - anorganické a organické gely. Biospecifická afinitní chromatografie - retence, bioafinitní sorbenty a podmínky chromatografie.
- 8. Příprava vzorku pro chromatografickou analýzu. Problém získání reprezentativního vzorku. Isolační a koncentrační techniky. Derivatizační metody v GC a HPLC.
- 9. Extrakční metody. Rovnováhy mezi dvěma kapalnými fázemi. Extrakce a roztřepávání. Superkritická extrakce. Voolba a vlastnosti superkritických mobilních fází. Příklady aplikací.
- 10. Destilace. Obecné principy. Prostá desilace. Rektifikace, pojem teoretického patra, faktory ovlivňující separační účinnost kolon, typy destilačních kolon. Destilace za snízeného tlaku, molekulová destilace, destilace s vodní parou, azeotropní destilace, extrakční destilace.
- 11. Krystalizace. Vymezení pojmu. Krystalizace z roztoku, vznik krystalizačních center a růst krystalu. Krystalizace z taveniny, zónové tavení. Krystalizační metody dělení enantiomerů, racemická sloučenina, racemát. Přímá krystalizace a dělení přes diastereomerní sloučeniny.
- 12. Membránové separační procesy. Membránové procesy v gradientu chemického potenciálu, dialýza, osmóza. Procesy v gradientu elektrického potenciálu, elektrodialýza, elektroosmóza. Procesy v gradientu tlaku, reversní osmóza, ultrafiltrace.
- 13. Elektroforetické metody. Základní pojmy, pohyblivost iontů, transportní děje. Doprovodné děje při elektroforetické separaci. Volná elektroforéza, Kapilární zónová elektroforéza, izotachoforéza.
- 14. Další separační metody. Sublimace. Vymezení pojmu. Různé techniky sublimace, gradientová sublimace, mrazová sublimace - lyofilizace. Sedimentační metody. Základní pojmy, sedimentační analýza.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Assessment methods (in Czech)
- Přednáška zakončená ústní zkouškou, popřípadě kolokviem.
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
C5440 Separation Methods
Faculty of ScienceAutumn 2005
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Chemistry Section – Faculty of Science - Timetable
- Wed 16:00–16:50 03021
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods are the main topics of the course; membrane separation processes and electromigration methods are mentioned as well.
- Syllabus (in Czech)
- 1. Chromatografické metody. Úvod, základní teorie a pojmy. Klasifikace chromatografických systémů a postupů. Základní teoretické modely popisující chromatografii. Retenční rovnice, teoretické patro, faktory ovlivňující separační účinnost, eluční poměr a rozlisení.
- 2. Plynová chromatografie. Základní pojmy. Van Deemterova rovnice.Technika GC. Blokové schema plynového chromatografu. Nosný plyn, techniky dávkování vzorku, náplňové a kapilární chromatografické kolony, stacionární fáze, enantioselektivní kolony.
- 3. Plynová chromatografie. Hlavní metody detekce pouzívané v GC. Plamenově ionizační detektor (FID), tepelně vodivostní detekce (TCD), elektronový záchyt (ECD), spojení GC s hmotnostní spektrometrem (GC-MS).
- 4. Plynová chromatografie. Kvalitativní analýza, identifikace z elučních údajů, eluční závislosti (Kovatsovy indexy), selektivní detektory. Kvantitavní analýza, faktory ovlivňující přesnost kvantitativní analýzy, metody kalibrace.
- 5. Kapalinová chromatografie. Základní pojmy. Chromatografie na sloupci, flash chromatografie - základy techniky, příprava kolon, detekce, stacionární fáze. Planární chromatografie. Papírová chromatografie, tenkovrstvá chromatografie.
- 6. Vysokoúčinná kapalinová chromatografie (HPLC). Blokové schema HPLC. Mobilní fáze, čerpadla, odplynění, gradient mobilní fáze. Dávkování vzorku. Kolony pro HPLC, stacionární fáze, výběr kolony, enantioselektivní fáze. Detektory pro HPLC, UV-VIS a fluorescenční detektor, refraktometr, ELSD, polarimetrická detekce.
- ) 7. Iontová, gelová a afinitní chromatografie. Klasifikace ionexů a jejich vlastnosti - selektivita ionexů. Rovnováhy při výměně iontů. Chelatační sorbenty. Vylučovací kapalinová chromatografie (GPC, SEC), retence v SEC, stacionární fáze - anorganické a organické gely. Biospecifická afinitní chromatografie - retence, bioafinitní sorbenty a podmínky chromatografie.
- 8. Příprava vzorku pro chromatografickou analýzu. Problém získání reprezentativního vzorku. Isolační a koncentrační techniky. Derivatizační metody v GC a HPLC.
- 9. Extrakční metody. Rovnováhy mezi dvěma kapalnými fázemi. Extrakce a roztřepávání. Superkritická extrakce. Voolba a vlastnosti superkritických mobilních fází. Příklady aplikací.
- 10. Destilace. Obecné principy. Prostá desilace. Rektifikace, pojem teoretického patra, faktory ovlivňující separační účinnost kolon, typy destilačních kolon. Destilace za snízeného tlaku, molekulová destilace, destilace s vodní parou, azeotropní destilace, extrakční destilace.
- 11. Krystalizace. Vymezení pojmu. Krystalizace z roztoku, vznik krystalizačních center a růst krystalu. Krystalizace z taveniny, zónové tavení. Krystalizační metody dělení enantiomerů, racemická sloučenina, racemát. Přímá krystalizace a dělení přes diastereomerní sloučeniny.
- 12. Membránové separační procesy. Membránové procesy v gradientu chemického potenciálu, dialýza, osmóza. Procesy v gradientu elektrického potenciálu, elektrodialýza, elektroosmóza. Procesy v gradientu tlaku, reversní osmóza, ultrafiltrace.
- 13. Elektroforetické metody. Základní pojmy, pohyblivost iontů, transportní děje. Doprovodné děje při elektroforetické separaci. Volná elektroforéza, Kapilární zónová elektroforéza, izotachoforéza.
- 14. Další separační metody. Sublimace. Vymezení pojmu. Různé techniky sublimace, gradientová sublimace, mrazová sublimace - lyofilizace. Sedimentační metody. Základní pojmy, sedimentační analýza.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Assessment methods (in Czech)
- Přednáška zakončená ústní zkouškou, popřípadě kolokviem.
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
C5440 Separation Methods
Faculty of ScienceAutumn 2004
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Chemistry Section – Faculty of Science - Timetable
- Wed 15:00–15:50 03021
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods are the main topics of the course; membrane separation processes and electromigration methods are mentioned as well.
- Syllabus (in Czech)
- 1. Chromatografické metody. Úvod, základní teorie a pojmy. Klasifikace chromatografických systémů a postupů. Základní teoretické modely popisující chromatografii. Retenční rovnice, teoretické patro, faktory ovlivňující separační účinnost, eluční poměr a rozlisení.
- 2. Plynová chromatografie. Základní pojmy. Van Deemterova rovnice.Technika GC. Blokové schema plynového chromatografu. Nosný plyn, techniky dávkování vzorku, náplňové a kapilární chromatografické kolony, stacionární fáze, enantioselektivní kolony.
- 3. Plynová chromatografie. Hlavní metody detekce pouzívané v GC. Plamenově ionizační detektor (FID), tepelně vodivostní detekce (TCD), elektronový záchyt (ECD), spojení GC s hmotnostní spektrometrem (GC-MS).
- 4. Plynová chromatografie. Kvalitativní analýza, identifikace z elučních údajů, eluční závislosti (Kovatsovy indexy), selektivní detektory. Kvantitavní analýza, faktory ovlivňující přesnost kvantitativní analýzy, metody kalibrace.
- 5. Kapalinová chromatografie. Základní pojmy. Chromatografie na sloupci, flash chromatografie - základy techniky, příprava kolon, detekce, stacionární fáze. Planární chromatografie. Papírová chromatografie, tenkovrstvá chromatografie.
- 6. Vysokoúčinná kapalinová chromatografie (HPLC). Blokové schema HPLC. Mobilní fáze, čerpadla, odplynění, gradient mobilní fáze. Dávkování vzorku. Kolony pro HPLC, stacionární fáze, výběr kolony, enantioselektivní fáze. Detektory pro HPLC, UV-VIS a fluorescenční detektor, refraktometr, ELSD, polarimetrická detekce.
- ) 7. Iontová, gelová a afinitní chromatografie. Klasifikace ionexů a jejich vlastnosti - selektivita ionexů. Rovnováhy při výměně iontů. Chelatační sorbenty. Vylučovací kapalinová chromatografie (GPC, SEC), retence v SEC, stacionární fáze - anorganické a organické gely. Biospecifická afinitní chromatografie - retence, bioafinitní sorbenty a podmínky chromatografie.
- 8. Příprava vzorku pro chromatografickou analýzu. Problém získání reprezentativního vzorku. Isolační a koncentrační techniky. Derivatizační metody v GC a HPLC.
- 9. Extrakční metody. Rovnováhy mezi dvěma kapalnými fázemi. Extrakce a roztřepávání. Superkritická extrakce. Voolba a vlastnosti superkritických mobilních fází. Příklady aplikací.
- 10. Destilace. Obecné principy. Prostá desilace. Rektifikace, pojem teoretického patra, faktory ovlivňující separační účinnost kolon, typy destilačních kolon. Destilace za snízeného tlaku, molekulová destilace, destilace s vodní parou, azeotropní destilace, extrakční destilace.
- 11. Krystalizace. Vymezení pojmu. Krystalizace z roztoku, vznik krystalizačních center a růst krystalu. Krystalizace z taveniny, zónové tavení. Krystalizační metody dělení enantiomerů, racemická sloučenina, racemát. Přímá krystalizace a dělení přes diastereomerní sloučeniny.
- 12. Membránové separační procesy. Membránové procesy v gradientu chemického potenciálu, dialýza, osmóza. Procesy v gradientu elektrického potenciálu, elektrodialýza, elektroosmóza. Procesy v gradientu tlaku, reversní osmóza, ultrafiltrace.
- 13. Elektroforetické metody. Základní pojmy, pohyblivost iontů, transportní děje. Doprovodné děje při elektroforetické separaci. Volná elektroforéza, Kapilární zónová elektroforéza, izotachoforéza.
- 14. Další separační metody. Sublimace. Vymezení pojmu. Různé techniky sublimace, gradientová sublimace, mrazová sublimace - lyofilizace. Sedimentační metody. Základní pojmy, sedimentační analýza.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Assessment methods (in Czech)
- Přednáška zakončená ústní zkouškou, popřípadě kolokviem.
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
C5440 Separation Methods
Faculty of ScienceAutumn 2003
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Chemistry Section – Faculty of Science - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods are the main topics of the course; membrane separation processes and electromigration methods are mentioned as well.
- Syllabus (in Czech)
- 1. Chromatografické metody. Úvod, základní teorie a pojmy. Klasifikace chromatografických systémů a postupů. Základní teoretické modely popisující chromatografii. Retenční rovnice, teoretické patro, faktory ovlivňující separační účinnost, eluční poměr a rozlisení.
- 2. Plynová chromatografie. Základní pojmy. Van Deemterova rovnice.Technika GC. Blokové schema plynového chromatografu. Nosný plyn, techniky dávkování vzorku, náplňové a kapilární chromatografické kolony, stacionární fáze, enantioselektivní kolony.
- 3. Plynová chromatografie. Hlavní metody detekce pouzívané v GC. Plamenově ionizační detektor (FID), tepelně vodivostní detekce (TCD), elektronový záchyt (ECD), spojení GC s hmotnostní spektrometrem (GC-MS).
- 4. Plynová chromatografie. Kvalitativní analýza, identifikace z elučních údajů, eluční závislosti (Kovatsovy indexy), selektivní detektory. Kvantitavní analýza, faktory ovlivňující přesnost kvantitativní analýzy, metody kalibrace.
- 5. Kapalinová chromatografie. Základní pojmy. Chromatografie na sloupci, flash chromatografie - základy techniky, příprava kolon, detekce, stacionární fáze. Planární chromatografie. Papírová chromatografie, tenkovrstvá chromatografie.
- 6. Vysokoúčinná kapalinová chromatografie (HPLC). Blokové schema HPLC. Mobilní fáze, čerpadla, odplynění, gradient mobilní fáze. Dávkování vzorku. Kolony pro HPLC, stacionární fáze, výběr kolony, enantioselektivní fáze. Detektory pro HPLC, UV-VIS a fluorescenční detektor, refraktometr, ELSD, polarimetrická detekce.
- ) 7. Iontová, gelová a afinitní chromatografie. Klasifikace ionexů a jejich vlastnosti - selektivita ionexů. Rovnováhy při výměně iontů. Chelatační sorbenty. Vylučovací kapalinová chromatografie (GPC, SEC), retence v SEC, stacionární fáze - anorganické a organické gely. Biospecifická afinitní chromatografie - retence, bioafinitní sorbenty a podmínky chromatografie.
- 8. Příprava vzorku pro chromatografickou analýzu. Problém získání reprezentativního vzorku. Isolační a koncentrační techniky. Derivatizační metody v GC a HPLC.
- 9. Extrakční metody. Rovnováhy mezi dvěma kapalnými fázemi. Extrakce a roztřepávání. Superkritická extrakce. Voolba a vlastnosti superkritických mobilních fází. Příklady aplikací.
- 10. Destilace. Obecné principy. Prostá desilace. Rektifikace, pojem teoretického patra, faktory ovlivňující separační účinnost kolon, typy destilačních kolon. Destilace za snízeného tlaku, molekulová destilace, destilace s vodní parou, azeotropní destilace, extrakční destilace.
- 11. Krystalizace. Vymezení pojmu. Krystalizace z roztoku, vznik krystalizačních center a růst krystalu. Krystalizace z taveniny, zónové tavení. Krystalizační metody dělení enantiomerů, racemická sloučenina, racemát. Přímá krystalizace a dělení přes diastereomerní sloučeniny.
- 12. Membránové separační procesy. Membránové procesy v gradientu chemického potenciálu, dialýza, osmóza. Procesy v gradientu elektrického potenciálu, elektrodialýza, elektroosmóza. Procesy v gradientu tlaku, reversní osmóza, ultrafiltrace.
- 13. Elektroforetické metody. Základní pojmy, pohyblivost iontů, transportní děje. Doprovodné děje při elektroforetické separaci. Volná elektroforéza, Kapilární zónová elektroforéza, izotachoforéza.
- 14. Další separační metody. Sublimace. Vymezení pojmu. Různé techniky sublimace, gradientová sublimace, mrazová sublimace - lyofilizace. Sedimentační metody. Základní pojmy, sedimentační analýza.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Assessment methods (in Czech)
- Přednáška zakončená ústní zkouškou, popřípadě kolokviem.
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5440 Separation Methods
Faculty of ScienceAutumn 2002
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Chemistry Section – Faculty of Science - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods are the main topics of the course; membrane separation processes and electromigration methods are mentioned as well.
- Syllabus (in Czech)
- 1. Chromatografické metody. Úvod, základní teorie a pojmy. Klasifikace chromatografických systémů a postupů. Základní teoretické modely popisující chromatografii. Retenční rovnice, teoretické patro, faktory ovlivňující separační účinnost, eluční poměr a rozlisení.
- 2. Plynová chromatografie. Základní pojmy. Van Deemterova rovnice.Technika GC. Blokové schema plynového chromatografu. Nosný plyn, techniky dávkování vzorku, náplňové a kapilární chromatografické kolony, stacionární fáze, enantioselektivní kolony.
- 3. Plynová chromatografie. Hlavní metody detekce pouzívané v GC. Plamenově ionizační detektor (FID), tepelně vodivostní detekce (TCD), elektronový záchyt (ECD), spojení GC s hmotnostní spektrometrem (GC-MS).
- 4. Plynová chromatografie. Kvalitativní analýza, identifikace z elučních údajů, eluční závislosti (Kovatsovy indexy), selektivní detektory. Kvantitavní analýza, faktory ovlivňující přesnost kvantitativní analýzy, metody kalibrace.
- 5. Kapalinová chromatografie. Základní pojmy. Chromatografie na sloupci, flash chromatografie - základy techniky, příprava kolon, detekce, stacionární fáze. Planární chromatografie. Papírová chromatografie, tenkovrstvá chromatografie.
- 6. Vysokoúčinná kapalinová chromatografie (HPLC). Blokové schema HPLC. Mobilní fáze, čerpadla, odplynění, gradient mobilní fáze. Dávkování vzorku. Kolony pro HPLC, stacionární fáze, výběr kolony, enantioselektivní fáze. Detektory pro HPLC, UV-VIS a fluorescenční detektor, refraktometr, ELSD, polarimetrická detekce.
- ) 7. Iontová, gelová a afinitní chromatografie. Klasifikace ionexů a jejich vlastnosti - selektivita ionexů. Rovnováhy při výměně iontů. Chelatační sorbenty. Vylučovací kapalinová chromatografie (GPC, SEC), retence v SEC, stacionární fáze - anorganické a organické gely. Biospecifická afinitní chromatografie - retence, bioafinitní sorbenty a podmínky chromatografie.
- 8. Příprava vzorku pro chromatografickou analýzu. Problém získání reprezentativního vzorku. Isolační a koncentrační techniky. Derivatizační metody v GC a HPLC.
- 9. Extrakční metody. Rovnováhy mezi dvěma kapalnými fázemi. Extrakce a roztřepávání. Superkritická extrakce. Voolba a vlastnosti superkritických mobilních fází. Příklady aplikací.
- 10. Destilace. Obecné principy. Prostá desilace. Rektifikace, pojem teoretického patra, faktory ovlivňující separační účinnost kolon, typy destilačních kolon. Destilace za snízeného tlaku, molekulová destilace, destilace s vodní parou, azeotropní destilace, extrakční destilace.
- 11. Krystalizace. Vymezení pojmu. Krystalizace z roztoku, vznik krystalizačních center a růst krystalu. Krystalizace z taveniny, zónové tavení. Krystalizační metody dělení enantiomerů, racemická sloučenina, racemát. Přímá krystalizace a dělení přes diastereomerní sloučeniny.
- 12. Membránové separační procesy. Membránové procesy v gradientu chemického potenciálu, dialýza, osmóza. Procesy v gradientu elektrického potenciálu, elektrodialýza, elektroosmóza. Procesy v gradientu tlaku, reversní osmóza, ultrafiltrace.
- 13. Elektroforetické metody. Základní pojmy, pohyblivost iontů, transportní děje. Doprovodné děje při elektroforetické separaci. Volná elektroforéza, Kapilární zónová elektroforéza, izotachoforéza.
- 14. Další separační metody. Sublimace. Vymezení pojmu. Různé techniky sublimace, gradientová sublimace, mrazová sublimace - lyofilizace. Sedimentační metody. Základní pojmy, sedimentační analýza.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Assessment methods (in Czech)
- Přednáška zakončená ústní zkouškou, popřípadě kolokviem.
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5440 Separation Methods
Faculty of ScienceAutumn 2001
- Extent and Intensity
- 1/0/0. 2 credit(s). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Chemistry Section – Faculty of Science - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods are the main topics of the course; membrane separation processes and electromigration methods are mentioned as well.
- Syllabus (in Czech)
- 1. Chromatografické metody. Úvod, základní teorie a pojmy. Klasifikace chromatografických systémů a postupů. Základní teoretické modely popisující chromatografii. Retenční rovnice, teoretické patro, faktory ovlivňující separační účinnost, eluční poměr a rozlisení.
- 2. Plynová chromatografie. Základní pojmy. Van Deemterova rovnice.Technika GC. Blokové schema plynového chromatografu. Nosný plyn, techniky dávkování vzorku, náplňové a kapilární chromatografické kolony, stacionární fáze, enantioselektivní kolony.
- 3. Plynová chromatografie. Hlavní metody detekce pouzívané v GC. Plamenově ionizační detektor (FID), tepelně vodivostní detekce (TCD), elektronový záchyt (ECD), spojení GC s hmotnostní spektrometrem (GC-MS).
- 4. Plynová chromatografie. Kvalitativní analýza, identifikace z elučních údajů, eluční závislosti (Kovatsovy indexy), selektivní detektory. Kvantitavní analýza, faktory ovlivňující přesnost kvantitativní analýzy, metody kalibrace.
- 5. Kapalinová chromatografie. Základní pojmy. Chromatografie na sloupci, flash chromatografie - základy techniky, příprava kolon, detekce, stacionární fáze. Planární chromatografie. Papírová chromatografie, tenkovrstvá chromatografie.
- 6. Vysokoúčinná kapalinová chromatografie (HPLC). Blokové schema HPLC. Mobilní fáze, čerpadla, odplynění, gradient mobilní fáze. Dávkování vzorku. Kolony pro HPLC, stacionární fáze, výběr kolony, enantioselektivní fáze. Detektory pro HPLC, UV-VIS a fluorescenční detektor, refraktometr, ELSD, polarimetrická detekce.
- 7. Iontová, gelová a afinitní chromatografie. Klasifikace ionexů a jejich vlastnosti - selektivita ionexů. Rovnováhy při výměně iontů. Chelatační sorbenty. Vylučovací kapalinová chromatografie (GPC, SEC), retence v SEC, stacionární fáze - anorganické a organické gely. Biospecifická afinitní chromatografie - retence, bioafinitní sorbenty a podmínky chromatografie.
- 8. Příprava vzorku pro chromatografickou analýzu. Problém získání reprezentativního vzorku. Isolační a koncentrační techniky. Derivatizační metody v GC a HPLC.
- 9. Extrakční metody. Rovnováhy mezi dvěma kapalnými fázemi. Extrakce a roztřepávání. Superkritická extrakce. Voolba a vlastnosti superkritických mobilních fází. Příklady aplikací.
- 10. Destilace. Obecné principy. Prostá desilace. Rektifikace, pojem teoretického patra, faktory ovlivňující separační účinnost kolon, typy destilačních kolon. Destilace za snízeného tlaku, molekulová destilace, destilace s vodní parou, azeotropní destilace, extrakční destilace.
- 11. Krystalizace. Vymezení pojmu. Krystalizace z roztoku, vznik krystalizačních center a růst krystalu. Krystalizace z taveniny, zónové tavení. Krystalizační metody dělení enantiomerů, racemická sloučenina, racemát. Přímá krystalizace a dělení přes diastereomerní sloučeniny.
- 12. Membránové separační procesy. Membránové procesy v gradientu chemického potenciálu, dialýza, osmóza. Procesy v gradientu elektrického potenciálu, elektrodialýza, elektroosmóza. Procesy v gradientu tlaku, reversní osmóza, ultrafiltrace.
- 13. Elektroforetické metody. Základní pojmy, pohyblivost iontů, transportní děje. Doprovodné děje při elektroforetické separaci. Volná elektroforéza, Kapilární zónová elektroforéza, izotachoforéza.
- 14. Další separační metody. Sublimace. Vymezení pojmu. Různé techniky sublimace, gradientová sublimace, mrazová sublimace - lyofilizace. Sedimentační metody. Základní pojmy, sedimentační analýza.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5440 Separation Methods
Faculty of ScienceAutumn 2000
- Extent and Intensity
- 1/0/0. 2 credit(s). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Chemistry Section – Faculty of Science - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods are the main topics of the course; membrane separation processes and electromigration methods are mentioned as well.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- KEIL, Bořivoj. Laboratorní technika organické chemie. Vyd. 2. Praha: Nakladatelství Československé akademie věd, 1963, 946 s. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5440 Separation Methods
Faculty of ScienceAutumn 1999
- Extent and Intensity
- 1/0/0. 2 credit(s). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Chemistry Section – Faculty of Science - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Organic Chemistry (programme PřF, M-CH)
- Organic Chemistry (programme PřF, N-CH)
- Syllabus
- Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods are the main topics of the course; membrane separation processes and electromigration methods are mentioned as well.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- KEIL, Bořivoj. Laboratorní technika organické chemie. Vyd. 2. Praha: Nakladatelství Československé akademie věd, 1963, 946 s. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course is taught annually.
The course is taught: every week.
C5440 Separation Methods
Faculty of ScienceAutumn 2011 - acreditation
The information about the term Autumn 2011 - acreditation is not made public
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Main objectives can be summarized as follows: Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods; membrane separation processes and electromigration methods are mentioned as well.
- Syllabus
- 1. Chromatography. Introduction, general theory and concepts. Main theoretical models. Retention equation, plate theory and kinetic theory. Elution ratio and resolution.
- 2. Gas chromatography. Van Deemter equation. GC techniques, block scheme of GC instruments. Carrier gas, sample injection, chomatographic columns, stationary phase. Enantioselective GC.
- 3. Main methods of GC detection. Flame Ionization Detector (FID), Thermal conductivity detector (TCD), Electron Capture Detector (ECD), GC-MS coupling.
- 4. Gas chromatography. Qualitative analysis, information from elution data, retention indexes (Kovats etc.). Selective detection. Quantitative analysis, calibration methods.
- 5. Liquid chromatography. Basic principles. Column chromatography, flash chromatography - main techniques, column preparation, detection, stationary phases. Planar chromatography - paper chromatography and TLC.
- 6. High performance liquid chromatography (HPLC). Block scheme of HPLC instrument. Mobile phase, pumps, degassing, gradient elution. Sample injection, HPLC columns, stationary phases. Enantioselective HPLC. HPLC detection - UV-Vis and fluorescence detectors, refractive index detector, evaporative light scattering detector. Chiroptical detection.
- 7. Ion exchange, gel permeation (size exclusion) and affinity chromatography. Ionexchangers and ion equilibria in IEC. Stationary phases and retention in SEC (GPC). Basic principles of affinity chromatography.
- 8. Sample preparation in chromatographic analysis. Derivatisation methods.
- 9. Extraction. Countercurrent extraction. Supercritical fluid extraction. MW assisted extraction.
- 10. Distillation. Basic principles. Rectification. Plate theory, distillation columns. Vacuum distillation, molecular distillation. Steam distillation, azeotropic and extractive distillation.
- 11. Crystallisation. Crystallisation from solution - nucleation and crystal growth. Crystallisation from melt. Resolution by means of crystallisation.
- 12. Sublimation. Sublimation techniques - gradient sublimation, lyophylization.
- 13. Electromigration separation methods. Basic principles, ion mobility, transport processes. Tiselius electrophoresis. Capillary electrophoresis. Isotachophoresis. SDS-PAGE.
- 14. Membrane permeation separation. Dialysis. Osmosis. Reverse osmosis and ultrafiltration.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Teaching methods
- Lectures
- Assessment methods
- Oral exam
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5440 Separation Methods
Faculty of ScienceAutumn 2010 - only for the accreditation
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Main objectives can be summarized as follows: Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods; membrane separation processes and electromigration methods are mentioned as well.
- Syllabus
- 1. Chromatography. Introduction, general theory and concepts. Main theoretical models. Retention equation, plate theory and kinetic theory. Elution ratio and resolution.
- 2. Gas chromatography. Van Deemter equation. GC techniques, block scheme of GC instruments. Carrier gas, sample injection, chomatographic columns, stationary phase. Enantioselective GC.
- 3. Main methods of GC detection. Flame Ionization Detector (FID), Thermal conductivity detector (TCD), Electron Capture Detector (ECD), GC-MS coupling.
- 4. Gas chromatography. Qualitative analysis, information from elution data, retention indexes (Kovats etc.). Selective detection. Quantitative analysis, calibration methods.
- 5. Liquid chromatography. Basic principles. Column chromatography, flash chromatography - main techniques, column preparation, detection, stationary phases. Planar chromatography - paper chromatography and TLC.
- 6. High performance liquid chromatography (HPLC). Block scheme of HPLC instrument. Mobile phase, pumps, degassing, gradient elution. Sample injection, HPLC columns, stationary phases. Enantioselective HPLC. HPLC detection - UV-Vis and fluorescence detectors, refractive index detector, evaporative light scattering detector. Chiroptical detection.
- 7. Ion exchange, gel permeation (size exclusion) and affinity chromatography. Ionexchangers and ion equilibria in IEC. Stationary phases and retention in SEC (GPC). Basic principles of affinity chromatography.
- 8. Sample preparation in chromatographic analysis. Derivatisation methods.
- 9. Extraction. Countercurrent extraction. Supercritical fluid extraction. MW assisted extraction.
- 10. Distillation. Basic principles. Rectification. Plate theory, distillation columns. Vacuum distillation, molecular distillation. Steam distillation, azeotropic and extractive distillation.
- 11. Crystallisation. Crystallisation from solution - nucleation and crystal growth. Crystallisation from melt. Resolution by means of crystallisation.
- 12. Sublimation. Sublimation techniques - gradient sublimation, lyophylization.
- 13. Electromigration separation methods. Basic principles, ion mobility, transport processes. Tiselius electrophoresis. Capillary electrophoresis. Isotachophoresis. SDS-PAGE.
- 14. Membrane permeation separation. Dialysis. Osmosis. Reverse osmosis and ultrafiltration.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Teaching methods
- Lectures
- Assessment methods
- Oral exam
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5440 Separation Methods
Faculty of ScienceAutumn 2007 - for the purpose of the accreditation
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- doc. RNDr. Ctibor Mazal, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Ctibor Mazal, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- Fundamentals and practice of basic separation methods used in organic chemistry (crystallization, distillation, sublimation, extraction, etc.) with focus on chromatographic methods are the main topics of the course; membrane separation processes and electromigration methods are mentioned as well.
- Syllabus (in Czech)
- 1. Chromatografické metody. Úvod, základní teorie a pojmy. Klasifikace chromatografických systémů a postupů. Základní teoretické modely popisující chromatografii. Retenční rovnice, teoretické patro, faktory ovlivňující separační účinnost, eluční poměr a rozlisení.
- 2. Plynová chromatografie. Základní pojmy. Van Deemterova rovnice.Technika GC. Blokové schema plynového chromatografu. Nosný plyn, techniky dávkování vzorku, náplňové a kapilární chromatografické kolony, stacionární fáze, enantioselektivní kolony.
- 3. Plynová chromatografie. Hlavní metody detekce pouzívané v GC. Plamenově ionizační detektor (FID), tepelně vodivostní detekce (TCD), elektronový záchyt (ECD), spojení GC s hmotnostní spektrometrem (GC-MS).
- 4. Plynová chromatografie. Kvalitativní analýza, identifikace z elučních údajů, eluční závislosti (Kovatsovy indexy), selektivní detektory. Kvantitavní analýza, faktory ovlivňující přesnost kvantitativní analýzy, metody kalibrace.
- 5. Kapalinová chromatografie. Základní pojmy. Chromatografie na sloupci, flash chromatografie - základy techniky, příprava kolon, detekce, stacionární fáze. Planární chromatografie. Papírová chromatografie, tenkovrstvá chromatografie.
- 6. Vysokoúčinná kapalinová chromatografie (HPLC). Blokové schema HPLC. Mobilní fáze, čerpadla, odplynění, gradient mobilní fáze. Dávkování vzorku. Kolony pro HPLC, stacionární fáze, výběr kolony, enantioselektivní fáze. Detektory pro HPLC, UV-VIS a fluorescenční detektor, refraktometr, ELSD, polarimetrická detekce.
- ) 7. Iontová, gelová a afinitní chromatografie. Klasifikace ionexů a jejich vlastnosti - selektivita ionexů. Rovnováhy při výměně iontů. Chelatační sorbenty. Vylučovací kapalinová chromatografie (GPC, SEC), retence v SEC, stacionární fáze - anorganické a organické gely. Biospecifická afinitní chromatografie - retence, bioafinitní sorbenty a podmínky chromatografie.
- 8. Příprava vzorku pro chromatografickou analýzu. Problém získání reprezentativního vzorku. Isolační a koncentrační techniky. Derivatizační metody v GC a HPLC.
- 9. Extrakční metody. Rovnováhy mezi dvěma kapalnými fázemi. Extrakce a roztřepávání. Superkritická extrakce. Voolba a vlastnosti superkritických mobilních fází. Příklady aplikací.
- 10. Destilace. Obecné principy. Prostá desilace. Rektifikace, pojem teoretického patra, faktory ovlivňující separační účinnost kolon, typy destilačních kolon. Destilace za snízeného tlaku, molekulová destilace, destilace s vodní parou, azeotropní destilace, extrakční destilace.
- 11. Krystalizace. Vymezení pojmu. Krystalizace z roztoku, vznik krystalizačních center a růst krystalu. Krystalizace z taveniny, zónové tavení. Krystalizační metody dělení enantiomerů, racemická sloučenina, racemát. Přímá krystalizace a dělení přes diastereomerní sloučeniny.
- 12. Membránové separační procesy. Membránové procesy v gradientu chemického potenciálu, dialýza, osmóza. Procesy v gradientu elektrického potenciálu, elektrodialýza, elektroosmóza. Procesy v gradientu tlaku, reversní osmóza, ultrafiltrace.
- 13. Elektroforetické metody. Základní pojmy, pohyblivost iontů, transportní děje. Doprovodné děje při elektroforetické separaci. Volná elektroforéza, Kapilární zónová elektroforéza, izotachoforéza.
- 14. Další separační metody. Sublimace. Vymezení pojmu. Různé techniky sublimace, gradientová sublimace, mrazová sublimace - lyofilizace. Sedimentační metody. Základní pojmy, sedimentační analýza.
- Literature
- SOJÁK, Ladislav. Separačné metódy v organickej chémii. 1. vyd. Bratislava: Univerzita Komenského, 1985, 240 s. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- POOLE, Colin F. and Salwa K. POOLE. Chromatography today. Amsterdam: Elsevier, 1991, 1026 s. ISBN 0-444-89161-7. info
- Kolektiv., Moderní separační metody, ČSAV Praha 1988.
- Assessment methods (in Czech)
- Přednáška zakončená ústní zkouškou, popřípadě kolokviem.
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
- Enrolment Statistics (recent)