C5900 Mass Spectrometry

Faculty of Science
Autumn 2008
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
prof. RNDr. Jana Klánová, Ph.D. (lecturer)
Mgr. Jiří Machát, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX - Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Timetable
Thu 7:00–8:50 kamenice
Prerequisites
Introductory course on Analytical Chemistry or an equivalent
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Introductory section treats in detail the contemporary instrumentation of mass spectrometry, principles of ionization, analysis and ion detection. Topics of ion fragmentation, compounds identification and quantitation follow. Finally, hyphenated techniques - GC/MS, HPLC/MS, CE/MS, and ICP/MS - are presented. Mail goals of this course: to obtain a survey about development and current state of methods and instrumentation of mass spectrometry; to use of MS for analysis of inorganic and organic compounds in the various type of samples.
Syllabus
  • I. History, principles of MS, fundamental terms.
  • II. Instrumentation. Sample introduction, vacuum system. Sample ionization, methods of ionization of volatiles / nonvolatiles compounds, soft and hard ionization techniques. Ion analysis, resolution, magnetic sector, electrostatic analysator, HRMS. TOF analysator and MALDI-TOF equipment. Ion cyclotron resonance. Linear quadrupole, ion trap. Tandem MS. Collisional activation. Ion detection. Spectrometer tunning.
  • III. Fragmentation. Metastable ions, nuclide ions, fundamental fragmentation mechanisms.
  • IV. Mass spectra and their use. Quantitation in MS.
  • V. Hyphenated techniques. Hyphenation with separation techniques GC/MS, HPLC/MS, CE/MS. Data acquisition and analysis. ICP/MS technique.
Literature
  • BARKER, J. Mass Spectrometry. 2nd Ed. Cichester: J. Wiley, 1999. Analytical Chemistry by Open Learning. ISBN 0 471 96762 9. info
  • BOEHM, S. and S. SMRČKOVÁ. Strukturní analýza organických sloučenin (Structure analysis of organic compounds). Praha: VŠCHT Praha, 1995. ISBN 80-7080-235-9. info
  • DE HOFFMAN, E. Tandem Mass Spectrometry: A Primer. Journal of Mass Spectrometry, John Wiley & Sons, Ltd., 1996, vol. 31, p. 129-138. ISSN 1076-5174. info
  • WONG, P. S. H. and R. G. COOKS. Ion Trap Mass Spectrometry. Current Separations, West Lafayette, USA: Bioanalytical Systems, Inc., 1997, vol. 16, p. 85. info
  • MCLAFFERTY, F.W. and F. TUREČEK. Interpretation of Mass Spectra. 4th ed. Sausalito , CA: University Science Book, 1993. ISBN 0-935702-25-3. info
  • KITSON, F. G., B. S. LARSEN and C. N. MCEWEN. Gas Chromatography and Mass Spectrometry, A Practical Guide. San Diego: Academic Press, 1996. ISBN 0-12-483385-3. info
Assessment methods
lectures, oral exam
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.
  • Enrolment Statistics (Autumn 2008, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2008/C5900