C5900 Mass Spectrometry

Faculty of Science
Autumn 2009
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
prof. RNDr. Jana Klánová, Ph.D. (lecturer)
Mgr. Jiří Machát, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX - Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Timetable
Wed 13:00–14:50 kamenice
Prerequisites
Introductory course on Analytical Chemistry or an equivalent
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course, students should be able to:
- define fundamentals of mass spectrometry and describe it in the contenxt with other spectral analytical methods;
- understand and clarify principles of instrumentation and functional layout of current ionization technics, mass analyzers and detecting elements;
- explain the mechanisms of ion fragmentation and dissociation using current ionization technics and fragmentation processes;
- asses and inerprete the mass spectra of common organic and anorganic compounds obtained by most frequently used ionization technics;
- evaluate significance of connection of mass spectrometry with various analytical technics, especially with separation technics GC/MS, HPLC/MS, CE/MS, ICP/MS;
- take advantage of mass spectroskopy in kvantitative and qualitative analyses of various types of samples;
Syllabus
  • I. History, principles of MS, fundamental terms.
  • II. Instrumentation. Sample introduction, vacuum system. Sample ionization, methods of ionization of volatiles / nonvolatiles compounds, soft and hard ionization techniques. Ion analysis, resolution, magnetic sector, electrostatic analysator, HRMS. TOF analysator and MALDI-TOF equipment. Ion cyclotron resonance. Linear quadrupole, ion trap. Tandem MS. Collisional activation. Ion detection. Spectrometer tunning.
  • III. Fragmentation. Metastable ions, nuclide ions, fundamental fragmentation mechanisms.
  • IV. Mass spectra and their use. Quantitation in MS.
  • V. Hyphenated techniques. Hyphenation with separation techniques GC/MS, HPLC/MS, CE/MS. Data acquisition and analysis. ICP/MS technique.
Literature
  • BARKER, J. Mass Spectrometry. 2nd Ed. Cichester: J. Wiley, 1999. Analytical Chemistry by Open Learning. ISBN 0 471 96762 9. info
  • BOEHM, S. and S. SMRČKOVÁ. Strukturní analýza organických sloučenin (Structure analysis of organic compounds). Praha: VŠCHT Praha, 1995. ISBN 80-7080-235-9. info
  • DE HOFFMAN, E. Tandem Mass Spectrometry: A Primer. Journal of Mass Spectrometry, John Wiley & Sons, Ltd., 1996, vol. 31, p. 129-138. ISSN 1076-5174. info
  • WONG, P. S. H. and R. G. COOKS. Ion Trap Mass Spectrometry. Current Separations, West Lafayette, USA: Bioanalytical Systems, Inc., 1997, vol. 16, p. 85. info
  • MCLAFFERTY, F.W. and F. TUREČEK. Interpretation of Mass Spectra. 4th ed. Sausalito , CA: University Science Book, 1993. ISBN 0-935702-25-3. info
  • KITSON, F. G., B. S. LARSEN and C. N. MCEWEN. Gas Chromatography and Mass Spectrometry, A Practical Guide. San Diego: Academic Press, 1996. ISBN 0-12-483385-3. info
Teaching methods
Education is performed as lectures with Powerpoint prezentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assesment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.
  • Enrolment Statistics (Autumn 2009, recent)
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